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Guo H.,Hebei University | Guo H.,Key Laboratory of Pharmaceutical Quality Control of Hebei Province | Wu F.,Hebei University | Zhang W.,Hebei University | Liu F.,Hebei University
Chinese Journal of Chromatography (Se Pu) | Year: 2013

The structure of polysaccharide in traditional Chinese medicine (TCM) is complex and characteristic. A method of oligosaccharide electrophoresis analysis assisted with cluster analysis (CA) was established to simultaneously identify TCMs. Six TCMs from three families were selected as experimental subjects and their polysaccharides were extracted. The obtained oligosaccharides via incompletely degraded TCM polysaccharides were derivatized using 1 -phen-yl-3-methyl-5-pyrazolone (PMP). The PMP- oligosaccharide derivatives were separated and analyzed by capillary zone electrophoresis (CZE). The six TCMs were identified by the featured information of oligosaccharide electropherograms with CA. The electrophoresis conditions were as follows: uncoated fused silica capillary column (49 cm/40 cm (total length/effective length) x 50 jjim); running buffer solution, 50 mmol/L phosphate buffer solution (pH 2.5); detection wavelength, 245 nm; operating voltage, 15 kV; hydrodynamic pressure injection, 10 cm ×4 s. The results showed that the six TCMs from three families were effectively identified by the method of TCM oligosaccharide electropherograms combined with CA. This method is a promising tool to identify TCM with good reliability and reproducibility.

Guo H.,Hebei University | Guo H.,Key Laboratory of Pharmaceutical Quality Control of Hebei Province | Pang X.,Hebei University | Zhang W.,Hebei University | And 2 more authors.
Analytical Methods | Year: 2013

The dissolution determination of multiple components in herbal preparations is a crucial method for controlling the quality of oral solid dosage forms. In order to improve the low detection sensitivity of dissolution samples, an efficient method for the extraction and concentration of multiple components in dissolution samples of Huoxiang Zhengqi tablets was developed by partitioned dispersive liquid-liquid microextraction (PDLLME). In the PDLLME process, 1.6 mL of THF as the disperser solvent and 100 μL of 1,2-dichloroethane as the extraction solvent were injected into 8 mL of the dissolution sample solution. Based on the partition coefficient, a portion of THF will transfer into the extraction solvent, then the multiple components in the Huoxiang Zhengqi tablet can be extracted into the mixture extraction phase of 1,2-dichloroethane and partitioned THF. In combination with HPLC-UV analysis, the enrichment factors reached to 43 to 119-fold for five of the target components (three of the more polar components were unknown). For honokiol and magnolol, a linear range from 10 to 1000 μg L-1 was obtained, and the recoveries at three spiking levels ranged from 90.2 to 99.4% with RSD less than 7.2% (n = 3). The proposed method may be applied for the extraction and concentration of multiple components in the dissolution samples of other herbal preparations. © 2013 The Royal Society of Chemistry.

Yan H.,Hebei University | Wang F.,Hebei University | Wang H.,Hebei University | Yang G.,Key Laboratory of Pharmaceutical Quality Control of Hebei Province
Journal of Chromatography A | Year: 2012

A simple, convenient and high selective miniaturized molecularly imprinted matrix solid-phase dispersion (mini-MI-MSPD) technique using l-tryptophan-imprinted polymers as sorbents was proposed for simultaneous extraction of four auxins (indole-3-acetic acid (IAA), indole-3-propionic acid (IPA), indole-3-butyric acid (IBA) and 1-naphthaleneacetic acid (NAA)) from orange samples coupled with high performance liquid chromatography-ultraviolet detection. The molecularly imprinted polymers (MIPs) synthesized using l-tryptophan as dummy template showed high affinity and selectivity to auxins, and so they were applied as the specific dispersant of MSPD to simultaneously eliminate the effect of template leakage on quantitative analysis. Under the optimized conditions, good linearity was obtained in a range of 0.02-2.00μgg-1 and the average recoveries of four auxins at three spiked levels ranged from 87.6% to 104.5% with the relative standard deviation (RSD) ≤4.4%. The presented mini-MI-MSPD method combined the advantages of MSPD for allowing the extraction, dispersion and homogenization in a single step and the advantages of MIPs for high affinity and selectivity towards four auxins, which could be applied to the determination of auxins in complicated vegetal samples. © 2012 Elsevier B.V.

Zhang X.,Hebei University | Chen J.,Hebei University | Mao M.,Hebei University | Guo H.,Hebei University | And 2 more authors.
International Journal of Biological Macromolecules | Year: 2014

The central composite design (CCD) was applied to optimize the water extraction of the polysaccharide from Adenophorae Radix in the paper. The three variables of extraction temperature (X1), extraction time (X2) and ratio of water to raw material (X3) were investigated by single factor analysis first. Since the presence of active polysaccharides and the imperfect of its extraction, the purpose of the paper was to evaluate the effects of selected variables on the yield of polysaccharide, which was expected to obtain the maximum yield. By variance and regression analysis, the quadratic regression equation was established as a predicted model. The R2 of 0.9825 indicated that the equation was well-fitted. The optimal conditions were 72.5°C, 133min, 1:35 (g/mL) and the predicted maximum yield of the polysaccharide was 5.78%. The predicted value was verified in triplicates under the optimum conditions, which was 5.68% and well matched with the predictive yield. © 2014 Elsevier B.V.

Chen B.-H.,Hebei University | Chen B.-H.,Key Laboratory of Pharmaceutical Quality Control of Hebei Province | Li J.-T.,Hebei University | Chen G.-F.,Hebei University | And 2 more authors.
Letters in Organic Chemistry | Year: 2012

Ultrasound-promoted synthesis of β-acetamido ketones catalyzed by cobalt sulfate heptahydrate via one-pot multi-component reaction coupling of aromatic aldehyde, acetophenone and acetyl chloride in acetonitrile was carried out in excellent yields at 25-28 °C, providing an efficient synthesis of these compounds. The use of ultrasound increased the rate of reactions compared with reactions under reflux condition. © 2012 Bentham Science Publishers.

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