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Liu C.-Y.,Capital Medical University | Yuan Z.,Key Laboratory of Otolaryngology Head and Neck Surgery | Yuan Z.,Beijing Institute of Otolaryngology | Han D.-M.,Capital Medical University | And 2 more authors.
Chinese Medical Journal | Year: 2010

Background Objective evaluation of allergic rhinitis (AR) requires in vivo and in vitro tests. In vitro tests are important to assist or ensure the main allergens in multi-allergen-sensitive patients. The aim of this study was to evaluate the utility of serum specific IgE (sIgE) in the diagnosis of AR patients with multi-allergens in the Chinese population. Methods Combining a positive skin prick test (SPT) and clinical history as the diagnostic reference criteria of AR, we estimated concentrations of sIgE produced in response to the 7 most frequent allergens among 85 AR patients, using the UniCAP assay system. Results Among 85 individuals with positive SPT results and allergen-specific nasal symptoms, sIgE concentration correlated well with SPT classes among all the tested allergens. Based on a clinical diagnosis and SPT results using a positive cut-off value of a class 1 score, the CAP test performed well and the sensitivity for different allergens ranged from 0.5 (giant ragweed) to 0.91 (Dermatophagoides pteronyssinus (Der p) and Dermatophagoides farinae (Der f), while specificity ranged from 0.93 (Der f) to 1.0 (animal hair, Der p and mugwort). When the cut-off score was adjusted to class 2, the sensitivity showed an increase overall while the remaining assessed items, including specificity, positive predictive value, negative predictive value and efficiency, showed an unacceptable decline. Conclusions Well-established serum sIgE tests correlated well with SPTs. Setting a class 1 cut-off for positivity of SPT results was better than a class 2 setting for assessing the AR diagnostic value. Source

Guo W.,Key Laboratory of Otolaryngology Head and Neck Surgery | Li G.-J.,University of Texas M. D. Anderson Cancer Center | Xu H.-B.,Key Laboratory of Otolaryngology Head and Neck Surgery | Xie J.-S.,Chinese National Human Genome Center | And 4 more authors.
Asian Pacific Journal of Cancer Prevention | Year: 2012

Background: Novel prognostic biomarkers or therapeutic molecular targets for laryngeal squamous cell carcinoma (LSCC) are an urgent priority. We here sought to identify multiple novel LSCC-associated genes. Methods: Using high-density microarray expression profiling, we identified multiple genes that were significantly altered between human LSCCs and paired normal tissues. Potential oncogenic functions of one such gene, DCUN1D5, were further characterized in vitro. Results: Our results demonstrated that DCUN1D5 was highly expressed in LSCCs. Overexpression of DCUN1D5 in vitro resulted in 2.7-fold increased cellular migration, 67.5% increased invasive capacity, and 2.6-fold increased proliferation. Endogenous DCUN1D5 expression was decreased in a time-dependent manner after genotoxic stress, and silencing of DCUN1D5 by siRNA decreased the number of cells in the S phase by 10.2% and increased apoptosis by 11.7%. Conclusion: Our data suggest that DCUN1D5 in vitro might have vital roles in DNA damage response, but further studies are warranted to assess its significance in vivo. Source

Qi B.,Key Laboratory of Otolaryngology Head and Neck Surgery | Liu B.,Key Laboratory of Otolaryngology Head and Neck Surgery | Krenmayr A.,University of Innsbruck | Liu S.,Key Laboratory of Otolaryngology Head and Neck Surgery | And 4 more authors.
Acta Oto-Laryngologica | Year: 2011

Conclusion: Not stimulating the apical cochlear region in tonal language speaking cochlear implantees significantly reduces discrimination of Mandarin vowels. The data presented here suggest that electrode arrays that allow complete cochlear coverage with stimulation pulses seem to be preferable over shorter arrays for use in cochlear implant (CI) indications. Objective: To assess the contribution of electrical stimulation beyond the first cochlear turn on tonal language speech perception. Methods: Twelve Mandarin-speaking users of the MED-EL COMBI 40 cochlear implant with complete insertion of the standard COMBI 40 electrode array participated in the study. Acute speech tests were performed in seven electrode configurations with stimulation either distributed over the whole length of the cochlea or restricted to the apical, middle or basal regions. The test battery comprised tone, consonant, and vowel identification in quiet as well as a sentence recognition task in quiet and noise. Results: While neither tone nor consonant identification depended crucially on the placement of the active electrodes, vowel identification and sentence recognition decreased significantly when the four apical electrodes were not stimulated. © Informa Healthcare. Source

Tan J.,Key Laboratory of Otolaryngology Head and Neck Surgery | Han D.,Key Laboratory of Otolaryngology Head and Neck Surgery | Wang J.,Key Laboratory of Otolaryngology Head and Neck Surgery | Liu T.,Key Laboratory of Otolaryngology Head and Neck Surgery | And 4 more authors.
European Archives of Oto-Rhino-Laryngology | Year: 2012

Our purpose is to simulate the airflow inside the healthy Chinese nose with normal nasal structure and function by computational fluid dynamics (CFD) method and to analyze the relationship between the airflow and physiological function. In this study, we used the software MIMICS 13.0 to construct 20 3-dimensional (3-D) models based on the computer tomography scans of Chinese adults' nose with normal nasal structure and function. Thereafter, numerical simulations were carried out using the software FLUENT 6.3. Then the characteristics of airflow inside the airway and sinuses were demonstrated qualitatively and quantitatively in steady state. We found that during the inhalation phase, the vortices and turbulences were located at anterior part and bottom of the nasal cavity. But there is no vortex in the whole nasal cavity during the expiratory phase. The distributions of pressure and wall shear stress are different in two phases. The maximum airflow velocity occurs around the plane of palatine velum during both inspiratory and expiratory phases. After the airflow passed the nasal valve, the peak velocity of inhaled airflow decreases and it increases again at the postnaris. Vice versa, the exhaled airflow decelerates after it passed the postnaris and it accelerates again at nasal valve. The data collected in this presentation validates the effectiveness of CFD simulation in the study of airflow in the nasal cavity. Nasal airflow is closely related to the structure and physiological functions of the nasal cavity. CFD may thus also be used to study nasal airflow changes resulting from abnormal nasal structure and nasal diseases. © 2011 Springer-Verlag. Source

Zhang N.,Key Laboratory of Otolaryngology Head and Neck Surgery | Liu S.,Key Laboratory of Otolaryngology Head and Neck Surgery | Xu J.,Key Laboratory of Otolaryngology Head and Neck Surgery | Liu B.,Key Laboratory of Otolaryngology Head and Neck Surgery | And 4 more authors.
Acta Oto-Laryngologica | Year: 2010

Conclusion: The study demonstrates that two methods of segmentation, i.e. word and character segmentations, produce equivalent results in the Mandarin Hearing in Noise Test (MHINT). Potentially, both methods of segmentation can be used clinically. A majority of the Mandarin-speaking cochlear implant subjects could complete MHINT with the more relaxed adaptive rules. The results make it possible to compare the performance of cochlear implant users across languages. Objectives: The primary purpose of the present study was to evaluate the modified adaptive scoring rules and to develop alternative methods of segmentations in MHINT that are suitable for the Chinese language. Methods: Thirty Mandarin-speaking normal-hearing adults were tested with MHINT using three adaptive rules based on character and/or word segmentation of the sentences. Twenty-three Mandarin-speaking post-lingually deafened cochlear implant patients were also recruited to participate in the testing. Results: There were no significant differences in the reception threshold for sentences and speech recognition scores obtained with either method of segmentation (p >0.05). Fifteen of the 23 cochlear implant subjects (65%) could be tested with the modified adaptive scoring rules. The performance-intensity functions of the cochlear implant subjects were shifted at least 8-10 dB to higher signal-to-noise ratios as compared with those of the normal-hearing subjects. © 2010 Informa UK Ltd. Source

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