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Ge S.,Key Laboratory of Oral Biomedicine of Shandong Province | Zhao N.,Key Laboratory of Oral Biomedicine of Shandong Province | Wang L.,Key Laboratory of Oral Biomedicine of Shandong Province | Yu M.,Key Laboratory of Oral Biomedicine of Shandong Province | And 5 more authors.
International Journal of Nanomedicine

Background: A nanohydroxyapatite-coated chitosan scaffold has been developed in recent years, but the effect of this composite scaffold on the viability and differentiation of periodontal ligament stem cells (PDLSCs) and bone repair is still unknown. This study explored the behavior of PDLSCs on a new nanohydroxyapatite-coated genipin-chitosan conjunction scaffold (HGCCS) in vitro as compared with an uncoated genipin-chitosan framework, and evaluated the effect of PDLSC-seeded HGCCS on bone repair in vivo. Methods: Human PDLSCs were cultured and identified, seeded on a HGCCS and on a genipin-chitosan framework, and assessed by scanning electron microscopy, confocal laser scanning microscopy, MTT, alkaline phosphatase activity, and quantitative real-time polymerase chain reaction at different time intervals. Moreover, PDLSC-seeded scaffolds were used in a rat calvarial defect model, and new bone formation was assessed by hematoxylin and eosin staining at 12 weeks postoperatively. Results: PDLSCs were clonogenic and positive for STRO-1. They had the capacity to undergo osteogenic and adipogenic differentiation in vitro. When seeded on HGCCS, PDLSCs exhibited significantly greater viability, alkaline phosphatase activity, and upregulated the bone-related markers, bone sialoprotein, osteopontin, and osteocalcin to a greater extent compared with PDLSCs seeded on the genipin-chitosan framework. The use of PDLSC-seeded HGCCS promoted calvarial bone repair. Conclusion: This study demonstrates the potential of HGCCS combined with PDLSCs as a promising tool for bone regeneration. © 2012 Cárdenas et al, publisher and licensee Dove Medical Press Ltd. Source

Cui J.,Shandong University | Liang J.,Shandong University | Wen Y.,Shandong University | Sun X.,Shandong University | And 6 more authors.
Journal of Biomedical Materials Research - Part A

Chitosan and β-glycerol phosphate (CS/β-GP) composite, with a thermosensitive sol-gel transition behavior, has been tested as one of the viable materials for barrier membrane fabrication. These studies have provided us with a new concept for a guided bone regeneration (GBR) membrane design. The composition, porous structure of the membrane, and the neutral mild preparation procedures make the CS/β-GP membrane a potentially active guide for bone regeneration. In this study, the CS/β-GP composite membrane, with different concentrations of β-GP, was studied to assess their potential utility in GBR application. The initial attachment of the ST2 stromal cell line to the CS/β-GP composite membrane was better than their attachment to the pure CS membrane. The proliferation and osteoblastic differentiation of the cells were much higher on the CS/β-GP composite membrane as compared to the pure CS membrane (p < 0.05). A mild inflammatory response was observed around the implanted CS/β-GP composite membrane without any foreign body reaction that continued up to 4 weeks of postsurgery. This primary study indicated that the in vitro and in vivo bioactivities of the CS/β-GP composite membrane fulfilled the requirements for GBR technique. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 2911-2917, 2014. © 2013 Wiley Periodicals, Inc. Source

Lin X.,Shandong University | Lin X.,Key Laboratory of Oral Biomedicine of Shandong Province | Kong J.,Shandong University | Kong J.,Key Laboratory of Oral Biomedicine of Shandong Province | And 6 more authors.
Mediators of Inflammation

Accumulating evidence from previous studies suggested that interleukin-1 (IL-1β) and tumor necrosis factor-α (TNF-α) play an important role in pathogenesis of temporomandibular disorders (TMD). However, the cell surface receptors and the intracellular signal pathways leading to these cytokines expression are not fully understood. In the current study, we investigated the roles of Toll-like receptor 4 (TLR4) and its adaptor myeloid differentiation factor 88 (MyD88) in the expression of IL-1β and TNF-α in synovial fibroblasts (SFs) separated from rat temporomandibular joint (TMJ) with lipopolysaccharide (LPS) stimulation. The results showed that treatment with LPS could increase TLR4, MyD88, IL-1β, and TNF-α expression at both mRNA and protein levels. In addition, increased expression of IL-1β and TNF-α could be blocked by treatment with TAK-242, a blocker of TLR4 signaling, and also by MyD88 inhibitory peptide (MIP). These findings suggested that maybe TLR4/MyD88 signal transduction pathway participates in enhanced expression of IL-1 and TNF-α in patients with TMD. The activation of TLR4/MyD88 signal transduction pathway which results in production of proinflammatory factors may play a role in the pathogenesis of TMD. © 2015 Xuefen Lin et al. Source

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