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Tan X.,Peoples Hospital | Sun S.,Peoples Hospital | Liu Y.,Zhongshan Ophthalmic Center | Zhu T.,Peoples Hospital | And 5 more authors.
Eye (Basingstoke) | Year: 2014

PurposeTo determine the levels of Th17-Associated cytokines, particularly interleukin (IL)-17 and IL-22 in tears of patients with dry eye syndrome.MethodsTear samples were collected from 20 healthy volunteers, 20 dry eye (DE) patients with non-Sjögren's syndrome (NSSDE) and 20 DE patients with Sjögren's syndrome (SSDE). Symptom questionnaire was self-Administered and multiple dry eye disease (DED)-related clinical tests were performed. The levels of IL-17 and IL-22 in tears were measured by enzyme-linked immunosorbent assay.ResultsThe levels of IL-17 and IL-22 were significantly increased in tears of DE patients compared with those of controls and also higher in SSDE patients compared with those of NSSDE patients (P<0.05). Moreover, the levels of IL-17 and IL-22 were positively correlated with questionnaire score and keratopathy score but negatively correlated with tear film break-up time and Schirmer I test in both NSSDE and SSDE patients (P<0.05).ConclusionsThe levels of IL-17 and IL-22 in tears were significantly increased in DE patients, which were associated with the disease severity. Therefore, Th17 cell-Associated cytokines, particularly IL-17 and IL-22, may have important roles in the immunopathogenesis of the DED. © 2014 Macmillan Publishers Limited All rights reserved.


Liu Y.-L.,Jiangnan University | Liu Y.-L.,Key Laboratory of Nuclear Medicine | Zou P.,Key Laboratory of Nuclear Medicine | Wu H.,Key Laboratory of Nuclear Medicine | And 3 more authors.
Acta Crystallographica Section C: Crystal Structure Communications | Year: 2012

The title compound, C16H24O10·0. 11H2O, is a key inter-mediate in the synthesis of 2-de-oxy-2-[ 18F]fluoro-d-glucose (18F-FDG), which is the most widely used mol-ecular-imaging probe for positron emission tomography (PET). The crystal structure has two independent mol-ecules (A and B) in the asymmetric unit, with closely comparable geometries. The pyran-ose ring adopts a 4 C 1 conformation [Cremer-Pople puckering parameters: Q = 0.553 (2) Å, = 16.2 (2)° and = 290.4 (8)° for mol-ecule A, and Q = 0.529 (2) Å, =15.3 (3)° and = 268.2 (9)° for mol-ecule B], and the dioxolane ring adopts an envelope conformation. The chiral centre in the dioxolane ring, introduced during the synthesis of the compound, has an R configuration, with the eth-oxy group exo to the manno-pyran-ose ring. The asymmetric unit also contains one water mol-ecule with a refined site-occupancy factor of 0.222 (8), which bridges between mol-ecules A and B via O - H⋯O hydrogen bonds. © 2012 International Union of Crystallography.


Liu L.,Jiangnan University | Ding H.,Key Laboratory of Nuclear Medicine | Huang H.B.,Key Laboratory of Nuclear Medicine
Applied Radiation and Isotopes | Year: 2016

Tracer kinetic modeling (TKM) is a promising quantitative method for physiological and biochemical processes in vivo. In this paper, we investigated the applications of an immune-inspired method to better address the issues of Simultaneous Estimation (SIME) of TKM with multimodal optimization. Experiments of dynamic FDG PET imaging experiments and simulation studies were carried out. The proposed artificial immune network (TKM_AIN) shows more scalable and effective when compared with the gradient-based Marquardt-Levenberg algorithm and the scholastic-based simulated annealing method. © 2015 Elsevier Ltd.


PubMed | Key Laboratory of Nuclear Medicine and Jiangnan University
Type: | Journal: Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine | Year: 2015

Tracer kinetic modeling (TKM) is a promising quantitative method for physiological and biochemical processes in vivo. In this paper, we investigated the applications of an immune-inspired method to better address the issues of Simultaneous Estimation (SIME) of TKM with multimodal optimization. Experiments of dynamic FDG PET imaging experiments and simulation studies were carried out. The proposed artificial immune network (TKM_AIN) shows more scalable and effective when compared with the gradient-based Marquardt-Levenberg algorithm and the scholastic-based simulated annealing method.


Zhang J.,Key Laboratory of Nuclear Medicine | Guo J.-Z.,Peoples Hospital of Jiangsu Province | Xiao H.-L.,Peoples Hospital of Jiangsu Province | Zhu L.,Key Laboratory of Nuclear Medicine | And 3 more authors.
World Journal of Gastroenterology | Year: 2010

AIM: To develop the simple, rapid and sensitive duallabel time-resolved fluoroimmunoassay for pepsinogens in human serum. METHODS: Based on two-site sandwich protocol, monoclonal antibodies (McAbs) against pepsinogen I (PG I) and PG II were co-coated in 96 microtitration wells, and tracer McAbs against PG II and PG II were labeled with europium (Eu) and samarium (Sm) chelate, respectively. Diluted serum samples of Eu3+- and Sm3+-McAbs were added into microtitration wells simultaneously. After washing, fluorescence of bound Sm3+ and Eu3+ tracers was detected. RESULTS: The detection limit was 0.2 μg/L for PG I and 0.05 μg/L for PG II. The assay range was 5.0-320.0 μg/L for PG I and 1.0-55.0 μg/L for PG II. The average recovery rate was 102.7% for PG II and 98.8% for PG II. Sera from healthy controls and patients with gastric disease were analyzed. The PG detected by dual-label assay was in good agreement with that detected by singlelabel assay or by enzyme-linked immunosorbent assay. CONCLUSION: Dual-label assay can provide highthroughput serological screening for gastric diseases. © 2010 Baishideng.


Zhou X.,Key Laboratory of Nuclear Medicine | Qin X.,Key Laboratory of Nuclear Medicine | Zhang J.,Key Laboratory of Nuclear Medicine
Acta Crystallographica Section E: Structure Reports Online | Year: 2010

The title compound, C14H12N2, was synthesized by the reaction of 4-methyl-N-(2-nitro-benz-yl)aniline with tin(II) chloride dihydrate in ethanol at 313 K. The indazole ring system is almost planar with a dihedral angle of 1.58 (10)° between the rings, whereas the plane of the attached p-tolyl substituent shows a dihedral angle of 46.26 (5)° with respect to the indazole core.


Xu J.,China Pharmaceutical University | Yuan H.,Key Laboratory of Nuclear Medicine | Ran T.,China Pharmaceutical University | Zhang Y.,China Pharmaceutical University | And 7 more authors.
Journal of Molecular Recognition | Year: 2015

Sodium-dependent glucose cotransporters (SGLTs) play an important role in glucose reabsorption in the kidney and have been identified as promising targets to treat diabetes. Because of the side effects like glucose and galactose malabsorption by targeting SGLT1, highly selective SGLT2 inhibitors are more promising in the treatment of diabetes. To understand the mechanism of selectivity, we conducted selectivity-based three-dimensional quantitative structure-activity relationship studies to highlight the structure requirements for highly selective SGLT2 inhibitors. The best comparative molecular field analysis and comparative molecular similarity indices analysis models showed the noncross-validated coefficient (r2) of 0.967 and 0.943, respectively. The predicted correlation coefficients (r2 pred) of 0.974 and 0.938 validated the reliability and predictability of these models. Besides, homology models of SGLT2 and SGLT1 were also constructed to investigate the selective mechanism from structure-based perspective. Molecular dynamics simulation and binding free energy calculation were performed on the systems of a potent and selective compound interacting with SGLT2 and SGLT1 to compare the different binding modes. The simulation results showed that the stretch of the methylthio group on Met241 had an essential effect on the different binding modes between SGLT1 and SGLT2, which was consistent with the three-dimensional quantitative structure-activity relationship analysis. Hydrogen bond analysis and binding free energy calculation revealed that SGLT2 binding complex was more stable and favorable than SGLT1 complex, which was highly correlated with the experimental results. Our obtained results give useful information for the investigation of the inhibitors' selectivity between SGLT2 and SGLT1 and will help for further development of highly selective SGLT2 inhibitors. © 2015 John Wiley & Sons, Ltd.


Zhu L.,University of Sydney | Wang K.,Key Laboratory of Nuclear Medicine | Zhou F.,University of Sydney | Zhu X.,Key Laboratory of Nuclear Medicine | And 3 more authors.
Folia Neuropathologica | Year: 2014

Ciliary neurotrophic factor (CNTF) is a neurocytokine, which could promote survival and/or differentiation in many cell types. In this study, the biological effects of CNTF on retinoic acid (RA)-predifferentiated SH-SY5Y neuroblastoma cells and the underlying molecular mechanism of this effect were investigated for the first time. The results showed that RA was able to increase cells susceptibility to CNTF via regulating the expression levels of CNTF receptors. A further study revealed that CNTF could induce phosphorylation of STAT3, Akt and ERK1/2 in RA-predifferentiated SH-SY5Y neuroblastoma cells, while the promoting activity of CNTF on survival and neurite growth of cells was attenuated by co-treatment with JAK2 inhibitor AG490 (25 μM), STAT3 inhibitor Curcumin (50 μM), PI3K inhibitor LY-294002 (50 μM), but not by co-treatment with MEK inhibitor PD98059 (50 μM). These findings suggested that JAK2/STAT3, as well as PI3K/Akt, play important roles in mediating the survival and neurite growth response of RA-predifferentiated cells to CNTF. Our study may be useful to further understand the functional role of CNTF and offer a convenient model to explore the therapeutic potential of CNTF in neurodegenerative diseases.


Qiu L.,Key Laboratory of Nuclear Medicine | Cheng W.,Key Laboratory of Nuclear Medicine | Lin J.,Key Laboratory of Nuclear Medicine | Luo S.,Key Laboratory of Nuclear Medicine | And 2 more authors.
Molecules | Year: 2011

A series of novel zoledronic acid (ZL) derivatives 1-hydroxy-3-(2-methyl- 1H-imidazol-1-yl)propane-1,1-diyldiphosphonic acid (MIPrDP), 1-hydroxy-4-(2-methyl-1H-imidazol-1-yl)butane-1,1-diyldiphosphonic acid (MIBDP), and 1-hydroxy-5-(2-methyl-1H-imidazol-1-yl)pentane-1,1-diyldiphosphonic acid (MIPeDP) were prepared and successfully labeled with 99mTc in high labeling yields. The in vitro stability and in vivo biodistribution of 99mTc-MIPrDP, 99mTc-MIBDP and 99mTc-MIPeDP were investigated and compared. The biodistribution studies indicate that the radiotracer 99mTc-MIPrDP has highly selective uptake in the skeletal system and rapid clearance from soft tissues. The present findings indicate that 99mTc-MIPrDP holds great potential for use in bone imaging.


Zhu X.,Key Laboratory of Nuclear Medicine | Wang K.,Key Laboratory of Nuclear Medicine | Zhang K.,Key Laboratory of Nuclear Medicine | Lin X.,Peoples Hospital of Jiangsu Province | And 2 more authors.
Journal of Biochemical and Molecular Toxicology | Year: 2015

Glutamate, the principal excitatory neurotransmitter, plays a central role in brain metabolism; however, aberrant neurotransmission of glutamate has been linked to neurodegenerative diseases. Therefore, the effective agents that target at glutamate-induced cell injury will be useful for prevention and treatment of neurodegenerative diseases. In this study, the neuroprotective effect of puerarin, an active isoflavone extracted from the Chinese herb Radix puerariae, against glutamate-induced cell injury in human neuroblastoma SH-SY5Y cells was evaluated for the first time. The results showed that the pretreatment of puerarin could attenuate glutamate-induced cell injury in a dose-dependent manner. This protective effect was mediated through inhibiting reactive oxygen species generation, attenuating the upregulation of Bax and downregulation of Bcl-2, preserving mitochondrial membrane potential (MMP), preventing cytochrome c release, and reducing caspase activity. These findings may significantly contribute to a better understanding of the neuroprotective effect of puerarin and provide new insights into its application toward neurodegenerative diseases in the future. © 2015 Wiley Periodicals, Inc.

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