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Tan X.,Peoples Hospital | Sun S.,Peoples Hospital | Liu Y.,Zhongshan Ophthalmic Center | Zhu T.,Peoples Hospital | And 5 more authors.
Eye (Basingstoke) | Year: 2014

PurposeTo determine the levels of Th17-Associated cytokines, particularly interleukin (IL)-17 and IL-22 in tears of patients with dry eye syndrome.MethodsTear samples were collected from 20 healthy volunteers, 20 dry eye (DE) patients with non-Sjögren's syndrome (NSSDE) and 20 DE patients with Sjögren's syndrome (SSDE). Symptom questionnaire was self-Administered and multiple dry eye disease (DED)-related clinical tests were performed. The levels of IL-17 and IL-22 in tears were measured by enzyme-linked immunosorbent assay.ResultsThe levels of IL-17 and IL-22 were significantly increased in tears of DE patients compared with those of controls and also higher in SSDE patients compared with those of NSSDE patients (P<0.05). Moreover, the levels of IL-17 and IL-22 were positively correlated with questionnaire score and keratopathy score but negatively correlated with tear film break-up time and Schirmer I test in both NSSDE and SSDE patients (P<0.05).ConclusionsThe levels of IL-17 and IL-22 in tears were significantly increased in DE patients, which were associated with the disease severity. Therefore, Th17 cell-Associated cytokines, particularly IL-17 and IL-22, may have important roles in the immunopathogenesis of the DED. © 2014 Macmillan Publishers Limited All rights reserved. Source


Liu L.,Jiangnan University | Ding H.,Key Laboratory of Nuclear Medicine | Huang H.B.,Key Laboratory of Nuclear Medicine
Applied Radiation and Isotopes | Year: 2016

Tracer kinetic modeling (TKM) is a promising quantitative method for physiological and biochemical processes in vivo. In this paper, we investigated the applications of an immune-inspired method to better address the issues of Simultaneous Estimation (SIME) of TKM with multimodal optimization. Experiments of dynamic FDG PET imaging experiments and simulation studies were carried out. The proposed artificial immune network (TKM_AIN) shows more scalable and effective when compared with the gradient-based Marquardt-Levenberg algorithm and the scholastic-based simulated annealing method. © 2015 Elsevier Ltd. Source


Zhu L.,University of Sydney | Wang K.,Key Laboratory of Nuclear Medicine | Zhou F.,University of Sydney | Zhu X.,Key Laboratory of Nuclear Medicine | And 3 more authors.
Folia Neuropathologica | Year: 2014

Ciliary neurotrophic factor (CNTF) is a neurocytokine, which could promote survival and/or differentiation in many cell types. In this study, the biological effects of CNTF on retinoic acid (RA)-predifferentiated SH-SY5Y neuroblastoma cells and the underlying molecular mechanism of this effect were investigated for the first time. The results showed that RA was able to increase cells susceptibility to CNTF via regulating the expression levels of CNTF receptors. A further study revealed that CNTF could induce phosphorylation of STAT3, Akt and ERK1/2 in RA-predifferentiated SH-SY5Y neuroblastoma cells, while the promoting activity of CNTF on survival and neurite growth of cells was attenuated by co-treatment with JAK2 inhibitor AG490 (25 μM), STAT3 inhibitor Curcumin (50 μM), PI3K inhibitor LY-294002 (50 μM), but not by co-treatment with MEK inhibitor PD98059 (50 μM). These findings suggested that JAK2/STAT3, as well as PI3K/Akt, play important roles in mediating the survival and neurite growth response of RA-predifferentiated cells to CNTF. Our study may be useful to further understand the functional role of CNTF and offer a convenient model to explore the therapeutic potential of CNTF in neurodegenerative diseases. Source


Zhou X.,Key Laboratory of Nuclear Medicine | Qin X.,Key Laboratory of Nuclear Medicine | Zhang J.,Key Laboratory of Nuclear Medicine
Acta Crystallographica Section E: Structure Reports Online | Year: 2010

The title compound, C14H12N2, was synthesized by the reaction of 4-methyl-N-(2-nitro-benz-yl)aniline with tin(II) chloride dihydrate in ethanol at 313 K. The indazole ring system is almost planar with a dihedral angle of 1.58 (10)° between the rings, whereas the plane of the attached p-tolyl substituent shows a dihedral angle of 46.26 (5)° with respect to the indazole core. Source


Zhang J.,Key Laboratory of Nuclear Medicine | Qin X.,Key Laboratory of Nuclear Medicine | Zhou X.,Key Laboratory of Nuclear Medicine
Acta Crystallographica Section E: Structure Reports Online | Year: 2010

In the title compound, C29H23N3, the pyrimidine ring adopts an envelope conformation. The dihedral angle between the phenyl rings attached to the pyrimidine-ring double bond is 62.09 (7)°. In the crystal, mol-ecules are linked by N - H⋯N hydrogen bonds, forming extended chains in the c-axis direction. Source

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