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Li B.,Key Laboratory of Veterinary Pharmaceutical Development | Li B.,Key Laboratory of New Animal Drug Project of Gansu Province | Li B.,Lanzhou Institute of Husbandry and Pharmaceutical science of CAAS | Zhang J.,Fulin Animal Science and Veterinary Medicine Officer | And 24 more authors.
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2015

A rapid and sensitive high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated to simultaneous quantify artesunate and its metabolite in sheep plasma. The plasma samples were prepared by liquid-liquid extraction. Chromatographic separation was achieved on a C18 column (250×4.6mm, 5μm) using methanol: water (60:40, v/v) (the water included 1mM ammonium acetate, 0.1% formic acid, and 0.02% acetic acid) as the mobile phase. Mass detection was carried out using positive electrospray ionization in multiple reaction monitoring mode. The calibration curve was linear from 1ng/mL to 400ng/mL (r2=0.9992 for artesunate, r2=0.9993 for its metabolite). The intra- and inter-day accuracy and precision were within the acceptable limits of ±10% at all concentrations for both artesunate and its metabolite. The recoveries ranged from 92% to 98% at the three concentrations for both. In summary, the LC-MS/MS metho described herein was fully successfully applied to pharmacokinetic studies of artesunate nanoemulsion after intramuscular delivery to sheep. © 2015. Source


Shang R.-F.,Key Laboratory of New Animal Drug Project of Gansu Province | Shang R.-F.,Chinese Academy of Agricultural Sciences | Liang J.-P.,Key Laboratory of New Animal Drug Project of Gansu Province | Liang J.-P.,Chinese Academy of Agricultural Sciences | And 2 more authors.
Zeitschrift fur Kristallographie - New Crystal Structures | Year: 2013

C33H46ClNO5S, orthorhombic, P2 12121 (no. 19), a = 10.9603(5) Å, b = 11.1542(3) Å, c = 30.7900(9) Å, V = 3764.2(2) Å3, Z = 4, Rgt(F) = 0.0600, wRref(F2) = 0.1379, T = 293 K. Source


Yi Y.-P.,Key Laboratory of New Animal Drug Project of Gansu Province | Yi Y.-P.,Lanzhou Institute of Husbandry and Pharmaceutical science of CAAS | Yang G.-Z.,Lanzhou University | Guo Z.-T.,Key Laboratory of New Animal Drug Project of Gansu Province | And 7 more authors.
Jiegou Huaxue | Year: 2015

A new pleuromutilin derivative, 14-O-[(4,6-diaminopyrimidine-2-yl) thioacetate] mutilin, was synthesized and structurally characterized by IR, NMR spectra and single-crystal X-ray diffraction. This compound contains a 5-6-8 tricyclic carbon skeleton and a pyrimidine ring. Its crystal is of orthorhombic system, space group P21 with a = 10.0237(6), b = 12.6087(7), c = 10.3749(8) A, β = 101.48(1)°, V = 1284.99(14) A3, Z = 2, F(000) = 540, Dc(Mg/m3) = 1.299, μ = 0.165 mm-1, R = 0.0649 and wR = 0.0797. The in vitro antibacterial activity study using Oxford cup assay showed this compound displayed more potent activity than pleuromutilin and similar antibacterial activity to that of tiamulin. Source


Wei X.,Key Laboratory of New Animal Drug Project of Gansu Province | Wei X.,Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture | Wei X.,Lanzhou Institute of Husbandryand Pharmaceutical science of CAAS | Wei X.,Lanzhou Institute of Husbandryand Pharmaceutical science of CAAS | And 20 more authors.
PLoS ONE | Year: 2015

A subchronic toxicity test was conducted in rats on the basis of a previous acute toxicity test to evaluate the safety of arecoline hydrobromide (Ah), to systematically study its pharmacological effects and to provide experimental support for a safe clinical dose. Eighty rats were randomly divided into four groups: a high-dose group (1000 mg/kg), medium-dose group (200 mg/kg), low-dose group (100mg/kg) and blank control group. The doses were administered daily via gastric lavage for 14 consecutive days. There were no significant differences in the low-dose Ah group compared to the control group (P>0.05) with regard to body weight, organ coefficients, hematological parameters and histopathological changes. The high-dose of Ah influenced some of these parameters, which requires further study. The results of this study indicated that a long-term, continuous high dose of Ah was toxic. However, it is safe to use Ah according to the clinically recommended dosing parameters. The level of Ah at which no adverse effects were observed was 100 mg/kg/day under the present study conditions. © 2015 Wei et al. Source


Li B.,Key Laboratory of Veterinary Pharmaceutical Development | Li B.,Key Laboratory of New Animal Drug Project of Gansu Province | Li B.,Lanzhou Institute of Husbandry and Pharmaceutical science of CAAS | Zhou X.-Z.,Key Laboratory of Veterinary Pharmaceutical Development | And 23 more authors.
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2014

A rapid and sensitive high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the determination of arecoline concentration in dog plasma. Plasma sample was prepared by protein precipitation using n-hexane (containing 1% isoamyl alcohol) with β-pinene as an internal standard. Chromatographic separation was achieved on an Agilent C18 column (4.6 × 75. mm, 3.5. μm) using methanol: 5. mM ammonium acetate as the mobile phase with isocratic elution. Mass detection was carried out using positive electrospray ionization in multiple reaction monitoring mode. The calibration curve for arecoline was linear over a concentration range of 2-500. ng/mL. The intra-day and inter-day accuracy and precision were within the acceptable limits of ±10% at all concentrations. In summary, the LC-MS/MS method described herein was fully validated and successfully applied to the pharmacokinetic study of arecoline hydrobromide tablets in dogs after oral administration. © 2014. Source

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