Key Laboratory of New Animal Drug Project of Gansu Province

Lanzhou, China

Key Laboratory of New Animal Drug Project of Gansu Province

Lanzhou, China
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Wang H.,Key Laboratory of New Animal Drug Project of Gansu Province | Wang H.,Chinese Academy of Sciences | Zhang X.,Gansu Agricultural University | Dong P.,Key Laboratory of New Animal Drug Project of Gansu Province | And 5 more authors.
International Journal of Pharmacology | Year: 2013

Microbial polysaccharides are located both inside the fungal cell walls and on the fungal cell surfaces and possess marked immunological properties. In this study, the extraction conditions of Saccharomyces cerevisiae polysaccharides (SCPS) were optimized using response surface methodology and the immune enhancement properties of SCPS were evaluated. The results indicated that the optimal extraction conditions were high pressure homogenization time of 35 min, ultrasonic power of 510 W and ultrasonic time of 26 min, respectively. Under these conditions, the maximal observed value extraction yield of S. cerevisiae polysaccharides (SCPS) was (29.84±0.09)%, which was agreed with predicted value 29.82%. Thin layer chromatography (TLC) exhibited SCPS may be a glucan. Pharmacological experiments indicated that SCPS could increase alkaline phosphatase (AKP), lysozyme (LZM), immunoglobulin A (IgA), immunoglobulin G (IgG) and Epidermal Growth Factor (EGF) levels in serum, increase secreted immunoglobulin A (SIgA) expression in jejunum secretion and decrease prostaglandin E2 (PGE2) expression in colon of immune-compromised rats at medium-dose. Saccharomyces cerevisiae polysaccharides has significant immune enhancement activity and could obviously protect intestine mucosa of immune-compromised rats. © 2013 Asian Network for Scientific Information.


Zhang S.,Chinese Academy of Agricultural Sciences | Zhang S.,Key Laboratory of Veterinary Pharmaceutics Discovery | Zhang S.,Key Laboratory of New Animal Drug Project of Gansu Province | Wang D.,Chinese Academy of Agricultural Sciences | And 11 more authors.
Journal of Ethnopharmacology | Year: 2013

Ethnopharmacological relevance Bai-Hu-Tang (BHT) was traditionally used to reduce fever heat and promote generation of body fluids. Aim of the study To investigate the effect and mechanism of BHT in the prevention of lipopolysaccharide (LPS) fever in manners of immune modulation. Materials and methods The model of fever syndrome of Chinese medicine pattern was imitated by LPS injection i.v. in rabbits, and BHT was gavaged. The serum levels of tumor necrosis factor-α (TNF-α), interleukin (IL-6, 10) and immunoglobulin (IgG, IgA, and IgM) were determined by enzyme-linked immunosorbent assay (ELISA); alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were tested by biochemical methods. Liver tissue damage was detected by hematoxylin-eosin (H&E) stain. Subpopulation of T cells was detected by Fluorescence Activated Cell Sorter (FACS). Genes expression of Toll-like receptor 4 (TLR4) and lipopolysaccharide binding protein (LBP) in liver tissue were assayed by real-time polymerase chain reaction (RT-PCR). Result The results demonstrated that BHT prevented sudden increase of IL-10, TNF-α, ALT and AST, and liver damage induced by LPS. BHT also prevented significant decrease of the percentage of CD8+ T cells since LPS injection. At the same time, BHT did not affect the gene expression of TLR4 and serum concentration of three immunoglobulins, which were increased by LPS, but made gene expression of LBP higher. Conclusion The results of this study indicated that BHT played an important role in immunity protection and anti-injury through preventing immunoinflammatory damage by LPS. The achievement thereby scientifically provided mechanism of BHT in the prevention of febrile disease, and supported its traditional use. © 2013 Elsevier Ireland Ltd.


Zhang S.,Chinese Academy of Agricultural Sciences | Zhang S.,Key Laboratory of Veterinary Pharmaceutics Discovery | Wang D.,Chinese Academy of Agricultural Sciences | Wang D.,Key Laboratory of Veterinary Pharmaceutics Discovery | And 6 more authors.
Journal of Ethnopharmacology | Year: 2015

Ethnopharmacological relevance Bai-Hu-Tang (BHT) has been traditionally used to clear heat and engender fluids. Aim of the study To reveal the alteration of differentially expressed genes (DEGs) between lipopolysaccharide (LPS) febrile syndrome in rabbits and treatment with BHT which is a classical anti-febrile formula in traditional Chinese medicine. Materials and methods Febrile model was induced by LPS injection (i.v.) in rabbits, and BHT was gavaged to another group of febrile rabbits. After sacrifice of animals, total RNA of liver tissue was isolated, processed, and hybridized to rabbit cDNA microarrays obtained from Agilent Co. The data of DEGs were obtained by lazer scanning and analyzed with Cluster program 3.0. Then bioinformatic analysis of DEGs was conducted through gene ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. In addition, expression levels of four relative genes were detected by quantitative real time ployenzyme chain reaction (qRT-PCR) to validate the accuracy of microarrays. Result The results demonstrated that genes expression pattern could be clustered into three groups significantly, and there were 606 up-regulated genes and 859 down-regulated genes in the model group, and 106 up-regulated genes and 429 down-regulated genes in BHT treated group. There were 286 DEGs existed as the common in two experimental groups. Enrichment analysis of GO annotations indicated that DEGs in model and BHT treated animals mainly referred catalytic activity and oxidoreductase activity for metabolic processes located in the membrane system at intracellular part, and binding activities increased significantly in treatment with BHT. Enrichment of KEGG analysis showed that the pathways of phagosome and protein processing in endoplasmic reticulum contained the most altered genes in the LPS group, but the percentage of phagosome pathway almost doubled in BHT group. Most DEGs involved in the LPS signal recognition system was up-regulated in LPS group, but partly decreased in BHT group. RT-PCR results of eight relative genes were consistent with the results of microarrays. Conclusion DEGs of LPS febrile syndrome mainly involved oxidoreductase and catalytic activity of the metabolic processes, and pathways of processing protein for pyrotoxin recognition; BHT mostly regulated the DEGs in the phagosome pathway to clear LPS in the liver, and partly interfered with gene expression in LPS recognition system. The study provided an important pioneering result on gene expression profiling research, and will facilitate the clinical care or further studies of the formula. © 2015 Elsevier Ireland Ltd.All rights reserved.


Zhang S.,Chinese Academy of Agricultural Sciences | Wang D.,Chinese Academy of Agricultural Sciences | Dong S.,Chinese Academy of Agricultural Sciences | Dong S.,Key Laboratory of New Animal Drug Project of Gansu Province | And 7 more authors.
Journal of Ethnopharmacology | Year: 2017

Ethnopharmacological relevance Bai-Hu-Tang (BHT), a classical anti-febrile Chinese formula comprising of liquorice, anemarrhena rhizome, gypsum and rice, has been traditionally used to anti-febrile treatment and promote the production of body fluid to relieve thirst. In this paper, we aim to explore anti-febrile mechanism of BHT at protein level through analyzing alteration of differentially expressed proteins (DEPs) both lipopolysaccharide (LPS) fever syndrome and that was treated with BHT in rabbits. Materials and methods Febrile model was induced by LPS injection (i.v.) in rabbits, and BHT (750 mg dry extract/kg body weight) was gavaged to another group of LPS fever rabbits. After sacrifice of animals, total protein of liver tissue was isolated, and two-dimensional liquid chromatography (LC) - tandem mass spectrometry (MS) coupled with isobaric tags for relative and absolute quantification (iTRAQ) labeling analysis was employed to quantitatively identify differentially expressed proteins in two group animals, which were compared with control group. Then bioinformatic analysis of DEPs was conducted through hierarchical Clustering, Venn analysis, gene ontology (GO) annotation enrichment, and kyoto encyclopedia of genes and genomes (KEGG) pathways enrichment. Result The results demonstrated there were 63 and 109 DEPs in LPS fever group and BHT-treated group, respectively. Enrichment analysis of GO annotations indicated that BHT mainly regulated expression of some extracellular structural proteins for response to stimulus and stress. KEGG analysis showed that ribosome and phagosome were the most significant pathways. Thereinto, several proteins in phagosome pathway were significantly up-regulated by BHT, including F-actin, coronin, Rac, and major histocompatibility complex class I (MHC I), which work in phagocytosis and cross-presentation Conclusion BHT may contribute to pyrogen clearance by boosting antigenic phagocytosis, degradation, and cross presentation in the liver. © 2017


Li B.,Key Laboratory of Veterinary Pharmaceutical Development | Li B.,Key Laboratory of New Animal Drug Project of Gansu Province | Li B.,Lanzhou Institute of Husbandry and Pharmaceutical science of CAAS | Zhang J.,Fulin Animal Science and Veterinary Medicine Officer | And 24 more authors.
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2015

A rapid and sensitive high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated to simultaneous quantify artesunate and its metabolite in sheep plasma. The plasma samples were prepared by liquid-liquid extraction. Chromatographic separation was achieved on a C18 column (250×4.6mm, 5μm) using methanol: water (60:40, v/v) (the water included 1mM ammonium acetate, 0.1% formic acid, and 0.02% acetic acid) as the mobile phase. Mass detection was carried out using positive electrospray ionization in multiple reaction monitoring mode. The calibration curve was linear from 1ng/mL to 400ng/mL (r2=0.9992 for artesunate, r2=0.9993 for its metabolite). The intra- and inter-day accuracy and precision were within the acceptable limits of ±10% at all concentrations for both artesunate and its metabolite. The recoveries ranged from 92% to 98% at the three concentrations for both. In summary, the LC-MS/MS metho described herein was fully successfully applied to pharmacokinetic studies of artesunate nanoemulsion after intramuscular delivery to sheep. © 2015.


Yi Y.-P.,Key Laboratory of New Animal Drug Project of Gansu Province | Yi Y.-P.,Lanzhou Institute of Husbandry and Pharmaceutical science of CAAS | Yang G.-Z.,Lanzhou University | Guo Z.-T.,Key Laboratory of New Animal Drug Project of Gansu Province | And 7 more authors.
Jiegou Huaxue | Year: 2015

A new pleuromutilin derivative, 14-O-[(4,6-diaminopyrimidine-2-yl) thioacetate] mutilin, was synthesized and structurally characterized by IR, NMR spectra and single-crystal X-ray diffraction. This compound contains a 5-6-8 tricyclic carbon skeleton and a pyrimidine ring. Its crystal is of orthorhombic system, space group P21 with a = 10.0237(6), b = 12.6087(7), c = 10.3749(8) A, β = 101.48(1)°, V = 1284.99(14) A3, Z = 2, F(000) = 540, Dc(Mg/m3) = 1.299, μ = 0.165 mm-1, R = 0.0649 and wR = 0.0797. The in vitro antibacterial activity study using Oxford cup assay showed this compound displayed more potent activity than pleuromutilin and similar antibacterial activity to that of tiamulin.


Li B.,Key Laboratory of Veterinary Pharmaceutical Development | Li B.,Key Laboratory of New Animal Drug Project of Gansu Province | Li B.,Lanzhou Institute of Husbandry and Pharmaceutical Science of CAAS | Zhou X.-Z.,Key Laboratory of Veterinary Pharmaceutical Development | And 23 more authors.
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2014

A rapid and sensitive high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the determination of arecoline concentration in dog plasma. Plasma sample was prepared by protein precipitation using n-hexane (containing 1% isoamyl alcohol) with β-pinene as an internal standard. Chromatographic separation was achieved on an Agilent C18 column (4.6 × 75. mm, 3.5. μm) using methanol: 5. mM ammonium acetate as the mobile phase with isocratic elution. Mass detection was carried out using positive electrospray ionization in multiple reaction monitoring mode. The calibration curve for arecoline was linear over a concentration range of 2-500. ng/mL. The intra-day and inter-day accuracy and precision were within the acceptable limits of ±10% at all concentrations. In summary, the LC-MS/MS method described herein was fully validated and successfully applied to the pharmacokinetic study of arecoline hydrobromide tablets in dogs after oral administration. © 2014.


Wei X.,Key Laboratory of New Animal Drug Project of Gansu Province | Wei X.,Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture | Wei X.,Lanzhou Institute of Husbandryand Pharmaceutical science of CAAS | Zhang J.,Key Laboratory of New Animal Drug Project of Gansu Province | And 14 more authors.
PLoS ONE | Year: 2015

A subchronic toxicity test was conducted in rats on the basis of a previous acute toxicity test to evaluate the safety of arecoline hydrobromide (Ah), to systematically study its pharmacological effects and to provide experimental support for a safe clinical dose. Eighty rats were randomly divided into four groups: a high-dose group (1000 mg/kg), medium-dose group (200 mg/kg), low-dose group (100mg/kg) and blank control group. The doses were administered daily via gastric lavage for 14 consecutive days. There were no significant differences in the low-dose Ah group compared to the control group (P>0.05) with regard to body weight, organ coefficients, hematological parameters and histopathological changes. The high-dose of Ah influenced some of these parameters, which requires further study. The results of this study indicated that a long-term, continuous high dose of Ah was toxic. However, it is safe to use Ah according to the clinically recommended dosing parameters. The level of Ah at which no adverse effects were observed was 100 mg/kg/day under the present study conditions. © 2015 Wei et al.


Shang R.-F.,Key Laboratory of New Animal Drug Project of Gansu Province | Shang R.-F.,Chinese Academy of Agricultural Sciences | Liang J.-P.,Key Laboratory of New Animal Drug Project of Gansu Province | Liang J.-P.,Chinese Academy of Agricultural Sciences | And 2 more authors.
Zeitschrift fur Kristallographie - New Crystal Structures | Year: 2013

C33H46ClNO5S, orthorhombic, P2 12121 (no. 19), a = 10.9603(5) Å, b = 11.1542(3) Å, c = 30.7900(9) Å, V = 3764.2(2) Å3, Z = 4, Rgt(F) = 0.0600, wRref(F2) = 0.1379, T = 293 K.

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