Key Laboratory of New Animal Drug Project

Lanzhou, China

Key Laboratory of New Animal Drug Project

Lanzhou, China
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Liu Y.,Key Laboratory of New Animal Drug Project | Han S.,Food Republic | Jiang Y.,Food Republic | Liang J.,Key Laboratory of New Animal Drug Project | And 4 more authors.
Polymer Composites | Year: 2015

Poly(lactic acid)/palygorskite (PA) nanocomposites were fabricated through the melt compounding. The significant finding by differential scanning calorimetry (DSC) showed that PA acted as a nucleating agent and accelerated the crystallization process of neat PLA. Consequently, a PLA crystallization peak appeared in all nanocomposites. With increasing PA content, the crystallization temperature increased from 92.1°C to 99.6°C. Dynamic Mechanical Analysis (DMA) indicated that the incorporation of PA hindered the motion of the PLA chains in the matrix, thereby increasing the maximum service temperature of the PLA. The storage modulus, G′, of the nanocomposite reached a plateau in the low frequency region which indicated formation of a network structure due to the increased interaction sites. The PLA/PA nanocomposites showed significant improvement in physical and thermal properties. © 2015 Society of Plastics Engineers.

Shang X.,Key Laboratory of New Animal Drug Project | Shang X.,Lanzhou Institute of Husbandry and Pharmaceutical science | Wang D.,Key Laboratory of New Animal Drug Project | Wang D.,Lanzhou Institute of Husbandry and Pharmaceutical science | And 10 more authors.
Parasites and Vectors | Year: 2014

Background: Psoroptes cuniculi can parasitise the ear canal of the rabbit, and cause the afflicted animals to cease feeding and become severely debilitated, sometimes resulting in death. In this study, we examined the oxidative status and inflammatory level of the peripheral blood of rabbits infested with Psoroptes cuniculi and investigated the pathogenesis of this disease. Methods. A total of 24 rabbits were divided into a healthy rabbit group and two infested rabbit groups. After weighing the rabbits, approximately 5 ml of blood was obtained from each animal. Then, the blood serum was extracted and used to assess the levels of antioxidant enzymes and inflammatory factors. Results: Compared to the healthy rabbits, the activities of catalase and glutathione-S-transferase and the level of malonyldialdehyde were increased, but the activity of superoxide dismutase was reduced in the infested rabbits. At the same time, a variety of inflammatory cells were activated, and the levels of inflammatory factors such as prostaglandin E2, interleukin-6, interleukin-8 and transforming growth factor-β1 were increased in peripheral blood. Conclusion: Animal acariasis was associated with immunosuppressive disorders and inflammatory reaction. These results advance our understanding of the pathogenesis of Psoroptes cuniculi infestation in rabbits and can help guide the effectual treatment of this disease in clinics. © 2014 Shang et al.; licensee BioMed Central Ltd.

Wang L.,Key Laboratory of New Animal Drug Project | Wang L.,Chinese Academy of Agricultural Sciences | Yang F.,Key Laboratory of New Animal Drug Project | Yang F.,Chinese Academy of Agricultural Sciences | And 12 more authors.
Journal of Animal and Veterinary Advances | Year: 2015

The objective of this study was to determine the prevalence of mastitis, identify the frequency alteration of predominant pathogens related to clinical and subclinical mastitis in Gansu and Ningxia Province of China. The 29 dairy farms comprising 2000 lactating cows enrolled from April 2012 to November 2014 of which 43.0% (860/2000) were found positive for mastitis by physical examination and Lanzhou Mastitis Test (LMT), the prevalence of Clinical Mastitis (CM) and Subclinical Mastitis (SCM) at cow level were 5.10 and 37.90%, respectively. The proportion of samples contained major pathogens were 97.60% and 871 isolates obtained from 860 milk samples. The common isolates from CM wereii. coli (26.89%), S. agalactiae (21.01%) and 5. aureus (19.33%) while from SCM were S. agalactiae, S. dysgalactiae and S. aureu, accounted for 30.45, 18.62 and 14.89%, respectively. The contagious pathogens had showed to predominate over the environmental pathogens and the Streptococcus sp. was found the predominant species which remains a significant cause of mastitis in local cattle herds. © Medwell Journals, 2015.

Yang A.,Lanzhou University of Technology | Yang A.,Key Laboratory of New Animal Drug Project | Li H.,Lanzhou University of Technology | Liu J.,Lanzhou University of Technology | And 2 more authors.
Advanced Materials Research | Year: 2013

Nine compounds were isolated from the petroleum ether extract of Euphorbia altotibetica. The structures of these compounds were elucidated as: squalene (1), β-sitosterol acetate (2), 11, 15, 19, 23-tetramethy-5, 9, 17-tetracosatrienoic acid (3), physcion (4), (z)-10-nonadecenoic acid (5), (z)-4-undecenoic acid (6), β-sitosterol (7), (24S)-stigmastan-4-en-3-one (8), naringenin (9). These compounds were identified on the basis of comparing their NMR datas with those of corresponding compounds in the literature. © (2013) Trans Tech Publications, Switzerland.

Qi S.,Shaanxi University of Technology | Zheng H.,Shaanxi University of Technology | Zheng H.,Key Laboratory of New Animal Drug Project | Zheng H.,Lanzhou Institute of Husbandry and Pharmaceutical science of CAAS
Journal of Animal and Veterinary Advances | Year: 2014

The intention of this study was to examine the effects of oral administration of L. acidophilus L3 on the number of intestinal mucosal immune cells of young bactrian camels. Young camels were fed daily withL. acidophilusL3 (a concentration of 2×109 CFUkg-1 feed) and their intestinal immune cells were assessed on day 28 by the histology, histochemistry and Cell Counting Methods. The number of Intraepithelial Lymphocytes (IELs), Goblet Cells (GCs), plasma cells and mast cells were counted, recorded and compared with the control group. Statistical analysis showed that the number of those intestinal mucosal immune cells were all increased in the probiotic group, compared with the control group and the difference was statistically significant (p<0.05). The distribution tendency of those cells in small intestine was that the number of intraepithelial lymphocytes, goblet cells and mast cells was gradually reduced from duodenum to ileum in two groups whereas the number of plasma cells was gradually increased from duodenum to ileum. The results indicated that L. acidophilus L3 has intense influence on the number of mucosal immune cells in small intestine of young camels, supplementation of the diet with L. acidophilus L3 is able to enhance the intestinal mucosal immunity of young camels. © Medwell Journals, 2014.

Jin G.,Lanzhou University of Technology | Yang Z.,Lanzhou University of Technology | Xue W.,Lanzhou University | Sheng J.,Lanzhou University of Technology | And 4 more authors.
Chinese Journal of Chemistry | Year: 2013

New isoconessimine derivatives were synthesized from conessine (1) and evaluated as acetylcholinesterase (AChE) inhibitors. The derivatives were prepared via two reaction steps, N-demethylation and nucleophilic substitution. All of the synthesized derivatives exhibited more potential anti- acetylcholinesterase activities than conessine (1) (IC50=16 μmol·L-1) and isoconessimine (2) (IC50>300 μmol·L-1). Compound 7b (3β-[methyl-[2-(4-nitrophenoxy) ethyl]amino]con-5-enine) showed the most potent inhibitory activity with an IC50 of 110 nmol/L which is close to that of reference compound huperzine A (IC50=70 nmol/L). The mode of AChE inhibition by 7b was reversible and non-competitive. In addition, molecular modeling was performed to explore the binding mode of inhibitor 7b at the active site of AChE and the results showed that 7b could be docked into the acetylcholinesterase active site and compound 7b had hydrophobic interactions with Trp279 and Leu282. A series of 3-N-aryloxyethyl substitutional isoconessimine derivatives were synthesized and evaluated as acetylcholinesterase (AChE) inhibitors. All of the synthesized derivatives exhibited potential anti-acetylcholinesterase activities with IC50 values at micromolar to sub-micromolar range. 7b showed the most potent inhibitory activity with an IC50of 110 nmol/L. The molecular docking results showed that 7b can be well docked into the active site of acetylcholinesterase. Copyright © 2013 SIOC, CAS, Shanghai & WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

PubMed | Key Laboratory of New Animal Drug Project
Type: Journal Article | Journal: BMC veterinary research | Year: 2016

Based on the prodrug principle, aspirin and eugenol, as starting precursors, were esterified to synthesize aspirin eugenol ester (AEE). The aim of the present study was to evaluate the antithrombotic effect of AEE in an animal disease model. In order to compare the therapeutic effects of AEE and its precursors, aspirin, eugenol and a combination of aspirin and eugenol were designed at the same molar quantities as the AEE medium dose in the control group.After oral administration of AEE (dosed at 18, 36 and 72mg/kg) for seven days, rats were treated with k-carrageenan to induce tail thrombosis. Following the same method, aspirin (20mg/kg), eugenol (18mg/kg) and 0.5% CMC-Na (30mg/kg) were administered as control drug. Different drug effects on platelet aggregation, hemorheology, TXB2/6-keto-PGF1 ratio and blood biochemistry were studied.AEE significantly inhibited ADP and AA-induced platelet aggregation in vivo. AEE also significantly reduced blood and plasma viscosity. Moreover, AEE down-regulated TXB2 and up-regulated 6-keto-PGF1, normalizing the TXB2/6-keto-PGF1 ratio and blood biochemical profile. In comparison with aspirin and eugenol, AEE produced more positive therapeutic effects than its precursors under the same molar quantity.It may be concluded that AEE was a good candidate for new antithrombotic and antiplatelet medicine. Additionally, this study may help to understand how AEE works on antithrombosis in different ways.

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