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Xiang M.,Huazhong University of Science and Technology | Xiang M.,Key Laboratory of Molecular Biophysics | Xiang M.,South-Central University for Nationalities | Su H.,Renmin University of China | And 4 more authors.
Journal of Medicinal Plants Research | Year: 2011

Polygonum amplexicaule D. Don (Polygonaceae) (Chinese name: Xuesanqi) is a widely distributed medicinal plant in China. It has been used in folk medicine to treat many common and frequentlyoccurring diseases, such as cardiovascular and cerebrovascular diseases, fractures, pain, etc. Thus, this plant has been of interest to researchers. However, the chemical constituents of Xuesanqi have not been investigated systematically. This study isolated and identified some effective compounds in this plant. The structure of important active chemicals of Xuesanqi from different regions was determined for the first time. Twelve phenolic compounds were isolated from this plant. Vanillin, isovanillic acid, phydroxyphenethyl alcohol, dihydro-kaempferol, and 5,7-dihydroxychromone were determined for the first time from Polygonum. As markers for quality standard of this herb, the content of methyl caffeate and ethyl caffeate was determined by HPLC. It was found that Xuesanqi from Wufeng, Lichuan, Badong Counties in Hubei Province and Jiujiang City of Jiangxi Province had higher content of both chemicals. The results suggest that the herb might be a potential source of phenolic compounds of an agent for improving blood circulation, treating various fractures, muscle and tissue swelling, and pain. © 2011 Academic Journals.

Zhao C.F.,Huazhong University of Science and Technology | Zhao C.F.,Key Laboratory of Molecular Biophysics | Li S.,Huazhong University of Science and Technology | Li S.J.,Huazhong University of Science and Technology | And 4 more authors.
Journal of Functional Foods | Year: 2013

Optimized solid-liquid extraction conditions by response surface methodology (RSM) and optimal solvent selectivity liquid-liquid fractionation by mixture design were studied for process of Pyracantha fortuneana fruits (PFF) healthy products. A combination of total polyphenolic content (TPC), total antioxidant activity (TAA) and chromatographic profiling was used to evaluate the optimization. RSM results were ethanol concentration, 71%; extraction temperature, 51. °C; and pH solution, 3.2. The mixture design indicated that the fraction with the highest TAA and TPC was directed to ethyl-acetate phase. The fractions of water-soluble and n-butylalcohol-soluble were rich in TAA and TPC compared with the starting row of PFF. Twenty-seven polyphenols were detected in chromatographic profilings, most of which were reported for the first time in this study. The improvements of accessibility in the optimized fractions suggest their flexible applicabilities for nutraceutical food industries. This study may be crucial approach for all similar natural functional product optimization. © 2013 Elsevier Ltd.

Xu H.,Huazhong University of Science and Technology | Zhao C.F.,Huazhong University of Science and Technology | Zhao C.F.,Key Laboratory of Molecular Biophysics | Tian G.G.,Huazhong University of Science and Technology | And 3 more authors.
Journal of Functional Foods | Year: 2016

Pyracantha fortuneana fruit is a potential source of nutraceutical and therapeutic agents. We investigated the effects of P. fortuneana fruit extract (PFE) on hyperlipidaemia, obesity, and oxidative stress in rodents. PFE significantly decreased body weight, triacylglycerol, total cholesterol, low-density lipoprotein-cholesterol, and malondialdehyde levels. By contrast, PFE elevated high-density lipoprotein-cholesterol levels, glutathione peroxidase, and superoxide dismutase activities in both preventive and therapeutic rodent groups. These results indicate that the amelioration of PFE on hyperlipidaemia and obesity was highly associated with the improvement of endogenous antioxidant activity. Polyphenols and saponins were quantified by spectrophotometric and HPLC methods. Several new saponin compounds were identified by liquid chromatography and tandem mass spectrometry as ursolic acid (UA) derivatives. The quercetin, rutin, and procyanidin treatments in HepG2 cells showed increases in low-density lipoprotein receptor activity and apolipoprotein-A1, but UA less affected these protein activities. Therefore, PFE could be promising in ameliorating chronic metabolic diseases via multiple constituent synergies. © 2016 Elsevier Ltd.

Li L.-Q.,Huazhong University of Science and Technology | Li L.-Q.,Key Laboratory of Molecular Biophysics | Li L.-Q.,Zhejiang University of Science and Technology | Li X.-L.,Huazhong University of Science and Technology | And 7 more authors.
Process Biochemistry | Year: 2013

Yields of paclitaxel decreased with repeated subculturing of Taxus media cells. We used minimal growth conservation and manipulation of genome methylation to sustain paclitaxel production by Taxus media cell cultures. The subculture period of Taxus cells can be prolonged to 180 d by incubating them at a low temperature (5 °C). Paclitaxel levels increased in the cells after conservation and during the first recovery subculture cycle, and then decreased during the subsequent recovery subculture cycle. Analysis of genetic variations in these cultures using amplified fragment-length polymorphism (AFLP) technology identified only two polymorphic bands associated with the second and sixth recovery cycle cultures. However, the results of high-performance liquid chromatography indicated that DNA methylation increased during the course of repeated subculturing. A decrease in DNA methylation level caused by treatment with 5-Aza-2′-deoxycytidine coincided with an increase in paclitaxel levels. Simultaneous exposure to both methyl jasmonate and 5-Aza-2′- deoxycytidine increased paclitaxel levels to 320.43 μg g-1 (dry weight), which is more than six times the paclitaxel content before conservation. To our knowledge, this is the first report about improving paclitaxel production by ensuring sustainable use of Taxus cells. © 2013 Elsevier Ltd. All rights reserved.

Li W.,Huazhong University of Science and Technology | Li W.,Key Laboratory of Molecular Biophysics | Chen W.-S.,Huazhong University of Science and Technology | Zhou P.-P.,Huazhong University of Science and Technology | And 3 more authors.
Chemical Engineering Journal | Year: 2013

One of the most promising biological sequestration technologies of CO2 is the enzyme catalyzed CO2 sequestration into stable and environmentally friendly mineral carbonates. The present manuscript focused on the biocatalytic precipitation of CaCO3 by extracellular carbonic anhydrase (CA) which was extracted and purified from the culture of Bacillus cereus. The kinetics of CaCO3 precipitation catalyzed by the bacterial CA in the presence of different enzyme concentrations was investigated through the gaseous diffusion system. The polymorph and morphology of CaCO3 crystals obtained in the precipitation process were also analyzed using XRD, FTIR and FESEM. The results showed that the change in the amount of deposited Ca2+ during the process of CaCO3 precipitation catalyzed by bacterial CA of different concentrations was well fitted with the exponential model. The enzyme concentrations of 0.2-2.0U/mL were beneficial to CaCO3 precipitation, however, overhigh enzyme concentration (8.0U/mL) was not conducive to CaCO3 precipitation. The integrated results of XRD, FTIR and FESEM analysis showed that there were significant differences in the morphologies of CaCO3 crystals among different enzyme concentrations. Vaterite was present at the lower concentration of CA, and the higher concentration of CA favored the formation of calcite. Therefore, different polymorphs and morphologies of CaCO3 crystals can be produced in the presence of different concentrations of CA. Furthermore, the role of bacterial CA in CaCO3 precipitation was related to the electrostatic adsorption of Ca2+ on CA enzyme protein and the preferential adsorption of CA enzyme protein on the crystal faces, in addition to the enzymatic catalysis. © 2013 Elsevier B.V.

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