The Key Laboratory of Molecular Animal Nutrition

Hangzhou, China

The Key Laboratory of Molecular Animal Nutrition

Hangzhou, China

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Hu C.H.,Zhejiang University | Hu C.H.,The Key Laboratory of Molecular Animal Nutrition | Xiao K.,Zhejiang University | Xiao K.,The Key Laboratory of Molecular Animal Nutrition | And 4 more authors.
Aquaculture Nutrition | Year: 2014

A total of 720 Nile tilapia fingerlings were allocated to four treatments and used to investigate the effects of zinc oxide supported on zeolite (Z-ZnO) on growth performance and intestinal function. The basal diet (control) was not supplemented with zinc and contained 60 mg Zn kg-1. The other three groups were added 30 mg Zn kg-1 to the basal diet, in which zinc was supplemented as Z-ZnO, Z + ZnO (mixture of zeolite and ZnO) or ZnSO4, respectively. As compared to the control, supplemental Z-ZnO improved (P < 0.05) specific growth rate, protein efficiency ratio (PER) and survivor ratio; increased (P < 0.05) villus-height-to-crypt-depth ratio and transepithelial electrical resistance; decreased (P < 0.05) mucosal-to-serosal flux of dextran 4 kDa; and improved (P < 0.05) intestinal activities of protease, amylase, lipase and alkaline phosphatase. Fish fed with Z-ZnO had higher (P < 0.05) specific growth rate and PER, and better intestinal barrier function than those fed with ZnSO4 or Z + ZnO. The zinc concentrations in whole body and vertebrae were not affected by dietary treatments. The results indicated that Z-ZnO improved intestinal morphology, barrier function and digestive enzyme activities. Such changes in intestinal health in the presence of Z-ZnO might contribute to its improvement in growth performance. © 2014 John Wiley & Sons Ltd.


Song Z.-H.,Zhejiang University | Song Z.-H.,The Key Laboratory of Molecular Animal Nutrition | Ke Y.-L.,Zhejiang University | Ke Y.-L.,The Key Laboratory of Molecular Animal Nutrition | And 8 more authors.
Journal of Animal Science | Year: 2015

The present study evaluated the beneficial effect of diosmectite–zinc oxide composite (DS-ZnO) on improving intestinal barrier restoration in piglets after acetic acid challenge and explored the underlying mechanisms. Twenty-four 35-d-old piglets (Duroc × Landrace × Yorkshire), with an average weight of 8.1 kg, were allocated to 4 treatment groups. On d 1 of the trial, colitis was induced via intrarectal injection of acetic acid (10 mL of 10% acetic acid [ACA] solution for ACA, DS-ZnO, and mixture of diosmectite [DS] and ZnO [DS+ZnO] groups) and the control group was infused with saline. Twenty-four hours after challenged, piglets were fed with the following diets: 1) control group (basal diet), 2) ACA group (basal diet), 3) DS-ZnO group (basal diet supplemented with DS-ZnO), and 4) DS+ZnO group (mixture of 1.5 g diosmectite [DS]/kg and 500 mg Zn/kg from ZnO [equal amount of DS and ZnO in the DS-ZnO treatment group]). On d 8 of the trial, piglets were sacrificed. The results showed that DS-ZnO supplementation improved (P < 0.05) ADG, ADFI, and transepithelial electrical resistance and decreased (P < 0.05) fecal scores, crypt depth, and fluorescein isothiocyanate-dextran 4kDa (FD4) influx as compared with ACA group. Moreover, DS-ZnO increased (P < 0.05) occludin, claudin-1, and zonula occluden-1 expressions; reduced (P < 0.05) caspase-9 and caspase-3 activity and Bax expression; and improved (P < 0.05) Bcl2, XIAP, and PCNA expression. Diosmectite–zinc oxide composite supplementation also increased (P < 0.05) TGF-β1 expression and ERK1/2 and Akt activation. These results suggest that DS-ZnO attenuates the acetic acid–induced colitis by improving mucosa barrier restoration, inhibiting apoptosis, and improving intestinal epithelial cells proliferation and modulation of TGF-β1 and ERK1/2 and Akt signaling pathway. © 2015 American Society of Animal Science. All rights reserved.

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