Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering

Laboratory of, China

Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering

Laboratory of, China
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Shen P.,Guangxi University | Shen P.,State Key Laboratory for Conservation and Utilization | Shen P.,Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering | Zhang J.,Guangxi University | And 11 more authors.
Bioresource Technology | Year: 2013

This study investigates the microbial community and structure in the internal circulation (IC) reactors that treat wastewater from bagasse spraying, with (reactor B) and without (reactor A) addition of molasses alcohol wastewater (MAW). The V3 regions in the 16S rRNA of bacteria were sequenced using illumina sequencing to characterize the microbial community structures. The results showed that there were approximately 34.8% more microorganisms were reduced, while the proportions of the three most predominant bacterial populations especially some sulfate-reducing bacteria increased in reactor B. The archaeal community composition was measured by PCR-DGGE (denaturing gradient gel electrophoresis) analysis and sequencing some clones from the 16S rRNA gene library. The results showed that numerous, mostly uncharacterized, archaeal genera are present in reactors A and B; the genus Methanomethylovorans was only detected in the samples that received MAW. This study demonstrated the significant effect of MAW on microbial communities in the wastewater treatment bioreactor. © 2013 Elsevier Ltd.


Tang M.,Guangxi University of Technology | Shen P.,Guangxi University of Technology | Shen P.,Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering | Shen P.,Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization | And 12 more authors.
Chinese Journal of Applied and Environmental Biology | Year: 2010

A put A gene was isolated from the genomic library of Sinorhizobium fredii HN01 by molecular and biotechnological methods, and it had 82% and 86% identities with the sequence of the proline dehydrogenase from S.meliloti 1021 at nucleotide and amico acid level, respectivly. With the suicide plasmid pK18mob, the polar mutant GXHNPB and non-polar mutant GXHNPA inactivated in putA gene were constructed through homologous recombination. The putA gene was cloned into pLAFRJ vetor resulting in a complemention plasmid pGXHN37. The complement strains GXHNPHA and GXHNPHB were constructed by transforming pGXH37 into host strains GXHNPA and GXHNPB, respectively. The results of growth test showed that there was no difference among HN01, mutant and complement strains when were cultivated on completemedium YMB, but the mutants showed no growth in the MM medium containing proline as the sole carbon and nitrogen source. Plant tests revealed that mutants had lower nodulation efficiency ability and nitrogen-fixation, and nodulated 1 day later on soybean compared with the wild-strain HN01. Fig 6, Tab 1, Ref 19.


Zhang W.,Guangxi University | Zhang W.,Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering | Zhang W.,Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization | Tian R.,Guangxi University | And 17 more authors.
Chinese Journal of Applied and Environmental Biology | Year: 2011

In order to know function of cysDN and its effect on plant nodulation, a random insertion mutant library of Sinorhizobium fredii 15142 was constructed by three parental hybridization with the plasmid pTnMod-RKm' as transposon vector. A mutant strain which could not metabolize sulfate while could use cysteine as sulfur source, was selected by MM medium with different sulfur sources from the library. Molecular cloning and sequence analysis showed that this operon had 92% and 96% similarity with the cysDN gene sequence of Sinorhizobium sp. BR816 at nucleotide and amino acid level, respectively. With the suicide plasmid pK18mob, the polar mutant cysDF/15142, cysNF/15142 and non-polar mutant cysDR/15142, cysNR/15142 inactivated in cysD and cysN were constructed through homologous recombination. A recombinant plasmid pLAFRJ+cysDN was constructed by using plasmid pLAFRJ ligated with cysDN, and transferred into above mutants to obtain complement strains. The results of growth tests found that the mutants had no growth on the MM medium with sulfate as sulfur source but the complement strains could grow normally, indicating that cysDN operon was related to sulfate assimilation. The plant tests indicated that the mutant strains delayed 1-2 d to nodulate than the wild strain, and nitrogenase activity and nodulation efficiency in mutants were slightly lower than those of the original strain, but there were no differences in the number and weight of nodules and the dry weight of plant.


Fan X.,Guangxi University | Fan X.,Key Laboratory of Ministry of Education for Microbial and Plant Genetic Engineering | Hu H.,Guangxi University | Huang G.,Guangxi University | And 5 more authors.
Plant and Soil | Year: 2015

Background and aims: Plant-growth promoting rhizobacteria (PGPR) can promote plant performance under water deficit, but the physiology and biochemistry of the promoting process induced by PGPR under different water deficits is not well known in maize (Zea mays L).Methods: A glasshouse study was conducted to determine the effects of Burkholderia sp. LD-11 on morphophysiological traits for plant growth and homeostasis between reactive oxygen species (ROS) and antioxidant enzymes under five regimens in two maize inbred lines.Results: Soil inoculation with Burkholderia sp. LD-11 promoted biomass accumulation and improved instantaneous water-use efficiency (WUEi), regardless of the soil water availability. It also triggered production of indole-3-acetic acid (IAA), decreasing the accumulation of abscisic acid (ABA) induced by the water deficit, alleviated ROS accumulation, and resulted in a reduction in lipid peroxidation induced by the water deficits. Soil inoculation also enhanced the tolerance to water deficit through reducing stomatal aperture by increasing the sensitivity of stomatal conductance (gs) to small changes in ABA concentration in the leaves.Conclusions: Soil inoculation with Burkholderia sp. LD-11 enhanced root systems and WUEi, offering a potential avenue for improving maize tolerance to water deficit. © 2015, Springer International Publishing Switzerland.

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