Zhu Y.,Key Laboratory of Ministry of Education for Industrial Biotechnology |
Zhu Y.,Jiangnan University |
Liu L.,Key Laboratory of Ministry of Education for Industrial Biotechnology |
Liu L.,Jiangnan University |
And 9 more authors.
Chinese Journal of Applied and Environmental Biology | Year: 2011
This work aimed to optimize the conditions of biotransformation of α-ketoisocaproate (KIC) by Rhodococcus opacus DSM 43250 via a statistical analysis-based approach. Firstly, Plackett-Burman design was adopted to evaluate six key factors which influenced α-ketoisocaproate transformation, and inoculum size, liquid volume and initial pH were determined to be the three key factors. Subsequently, the steepest ascent experiments were conducted to reach the optimal response area. Finally, a second-order polynomial model was constructed via Box-Behnken design and regression analysis by SAS software. The optimal conditions consisted of: Inoculum size 6.93%, liquid volume 33.38 mL, and initial pH 7.6. Under the optimized conditions, the maximal KIC production was predicted to be 31.08 mg/L, and in the validation experiments, KIC production reached 30.97 mg/L, which was 29.42% higher than that without optimization (23.93 mg/L). Fig 3, Tab 7, Ref 16. Source