Zhou X.,Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western China |
Zhou X.,Ningxia University |
Zhou X.,Swinburne University of Technology |
Yang C.,Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western China |
And 7 more authors.
Genetics and Molecular Research | Year: 2015
Berberine is a plant alkaloid with antimicrobial activity against a variety of microorganisms. In this study, the antimicrobial properties of berberine against multi-drug resistant field isolates of Staphylococcus epidermidis were investigated using berberine alone or in combination with a commonly used antibiotics in veterinary clinics, including penicillin, lincomycin, and amoxicillin. The results indicated that the minimum inhibitory concentrations of berberine, penicillin, lincomycin, and amoxicillin against field S. epidermidis isolates were 2-512, 0.8-213, 0.4-1024, and 0.4-256 mg/mL, respectively. Furthermore, the synergistic effects of antimicrobial activity against these multi-drug resistant isolates were observed when the berberine was combined with penicillin, lincomycin, or amoxicillin; no antagonistic effect of the combination was detected in any of the clinical isolates. These observations were further confirmed using a time-killing assay, in which a combination of 2 agents yielded a greater than 2.03-2.44 log10 decrease in colony-forming unit/mL compared with each agent alone. These findings suggest that berberine is a promising compound for preventing and treating multi-drug resistant S. epidermidis infected mastitis in dairy cows either alone or in combination with other commonly used antibiotics, such as penicillin, lincomycin, and amoxicillin. © FUNPEC-RP. Source
Jia Y.-Y.,Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western China |
Jia Y.-Y.,Ningxia University |
He J.-X.,Ningxia Medical University |
Sun Y.-F.,Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western China |
And 8 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2015
Background: Primary human lung epithelial cells are difficult to be isolated and cultured in vitro, which is characterized as limited sources, low cell viability, slow proliferation capacity, and lacking of differentiation capability. Objective: To establish an air-liquid interface model of lung epithelium by in vitro proliferation of human bronchiolar epithelial cells, which is used for research on function of lung epithelial cells. Methods: Primary human bronchiolar epithelial cells were isolated using Pronase and DNase I combined digestive methods, and then proliferated using medium containing ROCK kinase inhibitor. The proliferated cells were used for establishment of the air-liquid interface epithelium model. Cell differentiation was identified using scanning electron microscope, phase contrast microscope and immunofluorescent staining. Results And Conclusion: The primary human bronchiolar epithelial cells could be expanded successfully using medium containing ROCK kinase inhibitor, and the basal cell marker Cytokeratin14 was preferentially expressed in the proliferated cell population, indicating that these basal cells might be the main subpopulation of human lung epithelial stem cells. Subsequently, the proliferated cells under the air-liquid interface could differentiate into ciliated cells and non-ciliated column cells. The results suggest that the proliferation and differentiation of human bronchiolar epithelial cells were maintained in the presence of ROCK kinase inhibitor, and the air-liquid interface could promote the differentiation of human bronchiolar epithelial cells. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research, All Right Reserved. Source