Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry

Fengcheng, China

Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry

Fengcheng, China

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Liu X.X.,Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry | Liu F.,National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China | Gu W.,Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2013

To determine the content of four lignans in the fruits of Schisandra sphenanthera, Schisandra grandiflora, Schisandra rubriflora and Schisandra propinqua subsp. sinensis. The HPLC separation was performed on Diamonsil C18 (150 mm x 4.6 mm, 5 microm) columm using methanol-water (70 : 30) as the mobile phase at a flow rate of 0.8 mL/min, with the detection wavelength of 280 nm. Schisandrin, deoxyschizandrin and gamma-schizandrin were detected in four species of Schisandra. The content of schisantherin A in S. sphenanthera was measured up to the set standards of the Chinese Pharmacopoeia (2010 Edition). The content of deoxyschizandrin was high in S. grandiflora and S. rubriflora, and that of gamma-schizandrin was high in S. propinqua subsp. sinensis. The content of lignans is different in four species of Schisandra fruits in Qinling Mountains, it provides scientific reference for further study.


Qi X.-N.,Shaanxi Normal University | Mou Z.-L.,Shaanxi Normal University | Zhang J.,Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry | Zhang Z.-Q.,Shaanxi Normal University | Zhang Z.-Q.,Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry
Journal of Biomedical Materials Research - Part A | Year: 2014

Composite porous scaffolds have attracted extensive attention in the biomedical material field. The aim of this research was to prepare a novel tri-component composite porous scaffold and to evaluate its relevant properties. The porous scaffold was composed of chitosan (CS), silk fibroin (SF), and nanohydroxyapatite particles (nHA), which we named CS/SF/nHA scaffold and prepared via salt fractionation method combined with lyophilization. The porous structure was achieved using a porogen (salt), and the pore size was controlled by the size of porogen. To evaluate the characteristics of the tri-component scaffold, three bi-component scaffolds, CS/SF, CS/nHA, and SF/nHA, were simultaneously prepared for comparison. The scaffolds were subjected to morphological, micro-structural, and biodegradation analyses. Results demonstrated that all of the scaffolds had pore sizes of 100-300 μm and a porosity of 90.5-96.1%. The biodegradation characteristics of all scaffolds meet the requirements of good biomedical materials. The investigation of the mechanical properties showed that the tri-component scaffold has better properties than the bi-component scaffolds. The in vitro biocompatibility with osteoblast-like MG-63 cells showed that all the scaffolds are suitable for cell attachment and proliferation; however, the CS/SF/nHA composite porous scaffold is much more effective than the others. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 366-372, 2014. © 2013 Wiley Periodicals, Inc.


Mou Z.-L.,Shaanxi Normal University | Zhao L.-J.,Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry | Zhang Q.-A.,Shaanxi Normal University | Zhang J.,Shaanxi Normal University | And 2 more authors.
Journal of Supercritical Fluids | Year: 2011

Biocompatible three-dimensional scaffolds for cell culturing may facilitate methods for the repair of damaged human tissues. A novel hybrid porous scaffold of poly(lactic-co-glycolic acid), hydroxyapatite and collagen was prepared using a supercritical CO2 saturation technique. Expansion factors of scaffolds with different compositions were studied after supercritical CO 2 treatment to choose the optimal composition for three-dimensional culture. The supercritical CO2 process conditions, such as saturation temperature, saturation time and saturation pressure were varied to evaluate their influence on pore structure. The results showed that the pore size and porosity of the scaffold could be controlled by manipulating these process conditions. The porous samples were characterized by environmental scanning electron microscopy, energy-dispersive X-ray spectroscope, Fourier transform infrared spectroscopy and X-ray diffractometry. Finally, MG-63 cells were successfully cultured on the porous scaffold as assessed by electron and confocal microscopy, confirming the biocompatibility of this new hybrid porous scaffold. © 2011 Elsevier B.V. All rights reserved.


Xu M.,Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry | Wu S.,National Engineering Laboratory for Resource Development of Endangered Chinese Crude Drugs in Northwest of China | Liu X.-X.,Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry | Zhang W.,National Engineering Laboratory for Resource Development of Endangered Chinese Crude Drugs in Northwest of China | Gu W.,Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2013

OBJECTIVE: To analyse the genetic diversity of wild Schisandra sphenanthera in Qinling Mountains.METHODS: The genetic diversity of wild S. sphenanthera was investigated by SSR (Simple Sequences Repeats) markers.RESULTS: Nine pair primers generated a total of 61 bands 53 of which were polymorphic, and 6.8 genotypes were detected by each SSR primer pair on an average ranging from 5 to 11. The percentage of polymorphic band was 86.88%. The coefficient of genetic differentiation (G(st) = 0.3524) showed great genetic variation occurred within populations. Gene flow (N(m)) was 1.1850, which showed that gene flow occurred among populations. The highest genetic diversity of five populations was Yangxian population. And cluster analysis was based on genetic similarity coefficient. The five populations of wild S. sphenanthera samples were classified into two large groups, group I included Pingli population, Shanyang population and Ningqiang population; Group II included Taibai population and Yangxian population.CONCLUSION: The S. sphenanthera in Qinling Mountains, especially the regions of Hanzhong,Baoji and Shangluo in Shaanxi province, reveals high genetic diversity, which benefits the breeding of new varieties. It is necessary to protect the resource and ensure the sustainable development and utilization of it.


PubMed | National Engineering Laboratory for Resource Development of Endangered Chinese Crude Drugs in Northwest of China and Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry
Type: Journal Article | Journal: Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials | Year: 2014

To analyse the genetic diversity of wild Schisandra sphenanthera in Qinling Mountains.The genetic diversity of wild S. sphenanthera was investigated by SSR (Simple Sequences Repeats) markers.Nine pair primers generated a total of 61 bands 53 of which were polymorphic, and 6.8 genotypes were detected by each SSR primer pair on an average ranging from 5 to 11. The percentage of polymorphic band was 86.88%. The coefficient of genetic differentiation (G(st) = 0.3524) showed great genetic variation occurred within populations. Gene flow (N(m)) was 1.1850, which showed that gene flow occurred among populations. The highest genetic diversity of five populations was Yangxian population. And cluster analysis was based on genetic similarity coefficient. The five populations of wild S. sphenanthera samples were classified into two large groups, group I included Pingli population, Shanyang population and Ningqiang population; Group II included Taibai population and Yangxian population.The S. sphenanthera in Qinling Mountains, especially the regions of Hanzhong,Baoji and Shangluo in Shaanxi province, reveals high genetic diversity, which benefits the breeding of new varieties. It is necessary to protect the resource and ensure the sustainable development and utilization of it.

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