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Jiang X.,Hebei University | Zhang D.,Hebei University | Li X.,Hebei University | Li X.,Key Laboratory of Medicinal Chemistry and Molecular Diagnosis | And 7 more authors.
Journal of Pharmaceutical and Biomedical Analysis | Year: 2017

A novel polymer-based monolithic column was prepared via redox initiation system within the confines of a stainless steel column with 4.6 mm i.d. In the processes, hemin and lauryl methacrylate were used as co-monomers; ethylene dimethacrylate as crosslinking agent; n-butyl alcohol, ethanediol, and N, N-dimethylformamide as tri-porogens; benzoyl peroxide and N, N-dimethyl aniline as redox initiation system. The resulting polymer-based monolithic columns were characterized by scanning electron microscopy, nitrogen adsorption-desorption instrument, and mercury intrusion porosimeter, respectively. The results illustrated that the improved monolith had relative uniform porous structure, good permeability, and low back pressure. Aromatic compounds were used to test the chromatographic behavior of the monolith, resulting in highest column efficiency of 19 880 plates per meter with reversed-phase mechanism. Furthermore, the homemade monolith was used as the stationary phase of high performance liquid chromatography to separate proteins from complex bio-matrix, including human plasma, egg white, and snailase. The results showed that the monolithic column occupied good separation ability with these complex bio-samples. Excellent specific character of the homemade hemin-based monolith was that it could simultaneously remove high-abundance proteins (including human serum albumin, immunoglobulin G, and human fibrinogen) from human plasma and separate other proteins to different fractions. © 2017 Elsevier B.V.


Jiao X.,Ministry of EducationHebei UniversityBaoding071002 China | Yan H.,Key Laboratory of Medicinal Chemistry and Molecular Diagnosis
Journal of Applied Polymer Science | Year: 2017

A polymer monolithic column was prepared via redox system in a stainless steel column (50 × 4.6 mm i.d.) by using 1,6-hexanediol diacrylate as monomer and ethylene dimethacrylate as cross linking agent. Characteristics of the homemade monolithic column were investigated by scanning electron microscopy, nitrogen adsorption-desorption and mercury intrusion porosimeter, respectively. The results showed that the resulting monolithic column occupied relative uniform porous skeleton structure. Furthermore, the monolith was tested by separating aromatic compounds in conjunction of high performance liquid chromatography with the highest column efficiency of more than 21,000 plate per meter, which was relative high for polymer-based monolithic column with 4.6 mm i.d. At last, the monolithic column was used to simultaneously separate and quantitatively analyzes five active compounds in Chinese herb Rheum palmatum L. The validation of the method was carried out, which indicated that the accuracy represented by recovery was in the range of 98.48% to 101.57%. Limit of detection and limit of quantification for the five active compounds were less than 33.7 ng and 90.8 ng, respectively. All the results indicated that the present method was simple and efficient for quantitative analysis of the five active compounds from Chinese herb Rheum palmatum L. © 2017 Wiley Periodicals, Inc.


Wei H.M.,Hebei University | Zhao N.,Hebei University | Jiang N.,Hebei University | Duan W.F.,Hebei University | And 2 more authors.
Advanced Materials Research | Year: 2014

Perylene dicarboximide dimer with electron withdrawing groups were synthesized and fully characterized by 1H-NMR and 13C-NMR spectra. The effects of the substituted groups on the optical and electrochemical properties of the two compounds were investigated. The π-π* absorption peak of the perylene dicarboximide dimer compounds were red shifted from 525nm to 535nm. The band gaps of the dimer could be tuned from 2.28 eV to 2.13 eV. The first reduction wave of the dimer with electron withdrawing groups is shifted by 60 mV to more positive potential compared to that of perylene dicarboximide, suggesting the enhanced electron affinity. Furthemore, the dimer exhibited intermolecular aggregation, and decrease fluorescence quantum yield. © (2014) Trans Tech Publications, Switzerland.


Wang K.,Hebei University | Wang K.,Key Laboratory of Medicinal Chemistry and Molecular Diagnosis
Progress in Chemistry | Year: 2015

In nature, helical self-assembly by non-covalent interactions is a widely observed feature. Inspired by the unique features of fascinating chiral superstructures, chemists have paid more attention to design numerous helical supramolecular assemblies. In them, carbohydrates as a natural source of chirality have been widely investigated in the construction of chiral supramolecular assembly, which possessed potential application in materials, chemistry and biology. This review aims to overview of the chiral supramolecular assembly based on aromatic molecules-carbohydrate conjugates, including perylene bisimides, azobenzene, poly(p-phenylene), porphyrins, and so on. Their gel properties, supramolecular chirality and functionalization in the mixtures of organic solution and water, or in water, and the relationship between their supramolecular chirality and the type of the carbohydrates are described. Furthermore, the potential application and future development of the chiral supramolecular assembly based on carbohydrates are discussed. ©, 2015, Chinese Academy of Sciences. All right reserved.


Yang X.-L.,Key Laboratory of Medicinal Chemistry and Molecular Diagnosis | Yang X.-L.,Hebei University | Zhang S.,Hebei University | Zhu H.-J.,Key Laboratory of Medicinal Chemistry and Molecular Diagnosis | And 3 more authors.
Helvetica Chimica Acta | Year: 2011

Two new sesquiterpenoid esters, pestalotiopins B and C (1 and 2, resp.) related to the eremophilane class, together with one known compound, berkleasmin C (3), were isolated from the fermentation broth of the plant endophytic fungus Pestalotiopsis photiniae. Their structures and relative configurations were elucidated by extensive spectroscopic analysis including 1D- and 2D-NMR (HSQC, HMBC, and NOESY), and MS experiments. Compound 1 has an interesting lactam structure, and this type of sesquiterpenoid with a lactam structure is reported for the first time. Copyright © 2011 Verlag Helvetica Chimica Acta AG, Zürich, Switzerland.


Wang J.,Hebei University | Zhong S.,Hebei University | Duan W.,Hebei University | Gao B.,Hebei University | Gao B.,Key Laboratory of Medicinal Chemistry and Molecular Diagnosis
Tetrahedron Letters | Year: 2015

We report the synthesis and properties of perylene diimides with fluorinated substituents on the bay (BFPDIs). These BFPDIs exhibit good water solubility, high extinction coefficients, and high fluorescence quantum yields. Furthermore, these BFPDIs are used as probes in cellular imaging. © 2014 Elsevier Ltd. All rights reserved.


Wang K.-R.,Hebei University | Wang K.-R.,Key Laboratory of Medicinal Chemistry and Molecular Diagnosis | An H.-W.,Hebei University | Rong R.-X.,Hebei University | And 3 more authors.
Biosensors and Bioelectronics | Year: 2014

A new water-soluble glycocluster based on perylene bisimides PBI-12-Man has been designed and synthesized, and its specific and selective binding property with Concanavalin A (Con A) has been investigated by fluorescence spectroscopy and circular dichroism (CD) spectroscopy, which showed strong binding affinity for Con A with the binding constant of 8.2×105M-1 for monomeric mannose unit, two orders of magnitude higher than the corresponding monosaccharide ligand. Most interestingly, a fluorescence enhancement of PBI-12-Man was observed upon binding with Con A because of deaggregation of the self-assembly of PBI-12-Man induced by carbohydrate-protein interaction, and the further study of the fluorescence enhancement with macrophage cells showed that PBI-12-Man as a biocompatible agent had fluorescence imaging of the surface mannose receptor of the cells. Such fluorescence turn-on sensing of protein based on carbohydrate-protein interactions would facilitate the development of new protein-specific fluorescent probe for diagnosis and molecular imaging under live cell conditions. © 2014 Elsevier B.V.


PubMed | Shandong Academy of Sciences and Key Laboratory of Medicinal Chemistry and Molecular Diagnosis
Type: Journal Article | Journal: Glycoconjugate journal | Year: 2016

Two extracellular polysaccharides, designated as WPA and WPB, were isolated from the fungus Aspergillus aculeatus using Q-Sepharose fast flow and Sephacryl S-300 column chromatography. WPA composed of mannose and galactose in a molar ratio of 3.9:1.0, and WPB mainly contained mannose. The molecular weight of WPA and WPB was about 28.1kDa and 21.0kDa, respectively. On the basis of methylation and NMR analysis, the possible main chain of WPA was [5)--D-Galf-(12,6)--D-Manp(1], and WPB was mainly [2,6)--D-Manp(1], both with [-D-Manp(12)--D-Manp(12)--D-Manp(1] substituted at C-2 of [2,6)--D-Manp(1]. Meanwhile, WPA displayed a stronger anti-proliferative effect than WPB on HeLa, MCF-7 and MGC-803 cells in vitro. WPA and WPB could arrest HeLa cells in G2/M phase and induce HeLa cells apoptosis. Thus, our study provides evidence that WPA and WPB may be taken as potential candidates for treating cervical carcinoma.


Ren W.,Key Laboratory of Medicinal Chemistry and Molecular Diagnosis | Liu H.,Key Laboratory of Medicinal Chemistry and Molecular Diagnosis | Yang W.,Key Laboratory of Medicinal Chemistry and Molecular Diagnosis | Fan Y.,Key Laboratory of Medicinal Chemistry and Molecular Diagnosis | And 4 more authors.
Biosensors and Bioelectronics | Year: 2013

A versatile flow cytometric bead assay (CBA) is developed for sensitive DNA detection by integrating the advantages of hybridization chain reaction (HCR) for enzyme-free signal amplification, flow cytometry for robust and rapid signal readout as well as magnetic beads (MBs) for facile separation. In this HCR-CBA, a biotinylated hairpin DNA (Bio-H1) is firstly immobilized on streptavidin-functionalized MBs. Upon the addition of target DNA, each target would hybridize with one Bio-H1 to open its hairpin structure and subsequently initiate a cascade of hybridization events between two species of fluorescent DNA hairpin probes (H1*/H2*) to form a nicked double helical DNA structure, resulting in amplified accumulation of numerous fluorophores on the MBs. Finally, the fluorescent MBs are directly analyzed by flow cytometry. This technique enables quantitative analysis of the HCR products anchored on the MBs as a function of target DNA concentration, and analysis of each sample can be completed within few minutes. Therefore, the HCR-CBA approach provides a practical DNA assay with greatly improved sensitivity. The detection limit of a model DNA target is 0.5. pM (3. σ), which is about 3 orders of magnitude lower compared with traditional hybridization methods without HCR. Furthermore, the signal of complementary target can be clearly distinguished from that of single-base mismatched sequences, indicating the high specificity of the HCR-CBA. Moreover, this strategy is also successfully applied to the DNA analysis in complex biological samples, showing great potential in gene analysis and disease diagnosis in clinical samples. © 2013 Elsevier B.V.


PubMed | Key Laboratory of Medicinal Chemistry and Molecular Diagnosis
Type: | Journal: Applied biochemistry and biotechnology | Year: 2016

An extracellular polysaccharide TP1A was purified from the fermented broth of Trichoderma sp. KK19L1 by combination of Q Sepharose fast flow and Sephacryl S-300 chromatography. TP1A was composed of Man, Gal, and Glc in a molar ratio of about 3.0:5.1:8.1. The molar mass of TP1A was about 40.0kDa. Methylation and NMR analysis indicated that the probable structure of TP1A was [4,6)--D-Glcp(16)--D-Galf(16)--D-Galf(12,6)--D-Galf(12,6)--D-Galf(12,6)--D-Galf(12,6)--D-Manp(12,6)--D-Manp(1] with [-D-Glcp(1] and [-D-Manp(16)--D-Glcp(16)--D-Glcp(1] as branches. The antitumor study showed that TP1A was able to inhibit the cell viability of HeLa and MCF-7 cells. TP1A could arrest HeLa cells in G2/M phase and induce HeLa cell apoptosis. These findings suggest that fungal polysaccharides could be a potential source for antitumor agents.

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