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Wang W.,Third Institute of Oceanography | Wang W.,State Key Laboratory Breeding Base of Marine Genetic Resources | Wang W.,Key Laboratory of Marine Genetic Resources of Fujian Province | Zhong R.,Third Institute of Oceanography | And 9 more authors.
Applied Microbiology and Biotechnology | Year: 2014

Indigenous oil-degrading bacteria play an important role in efficient remediation of polluted marine environments. In this study, we investigated the diversity and abundance of indigenous oil-degrading bacteria and functional genes in crude oil-contaminated seawater of the Dalian coast. The gene copy number bacterial 16S rRNA in total were determined to be about 1010 copies L-1 in contaminated seawater and 109 copies L -1 in uncontaminated seawater. Bacteria of Alcanivorax, Marinobacter, Novosphingobium, Rhodococcus, and Pseudoalteromonas were found to be predominant oil-degrading bacteria in the polluted seawater in situ. In addition, bacteria belonging to Algoriphagus, Aestuariibacter, Celeribacter, Fabibacter, Zobellia, Tenacibaculum, Citreicella, Roseivirga, Winogradskyella, Thioclava, Polaribacter, and Pelagibaca were confirmed to be the first time as an oil-degrading bacterium. The indigenous functional enzymes, including AlkB or polycyclic aromatic hydrocarbons ring-hydroxylating dioxygenases α (PAH-RHDα) coding genes from Gram-positive (GP) and Gram-negative bacteria (GN), were revealed and quite diverse. About 1010 to 1011 copies L-1 for the expression of alkB genes were recovered and showed that the two-thirds of all the AlkB sequences were closely related to widely distributed Alcanivorax and Marinobacter isolates. About 109 copies L-1 seawater for the expression of RHDαGN genes in contaminated seawater and showed that almost all RHDαGN sequences were closely related to an uncultured bacterium; however, RHDαGP genes represented only about 105 copies L-1 seawater for the expression of genes in contaminated seawater, and the naphthalene dioxygenase sequences from Rhodococcus and Mycobacterium species were most abundant. Together, their data provide evidence that there exists an active aerobic microbial community indigenous to the coastal area of the Yellow sea that is capable of degrading petroleum hydrocarbons. © 2014 Springer-Verlag. Source


Jiang L.,Third Institute of Oceanography | Jiang L.,Key Laboratory of Marine Genetic Resources of Fujian Province | Jiang L.,Xiamen University | Long C.,Xiamen University | And 6 more authors.
International Journal of Hydrogen Energy | Year: 2014

A unique thermophilic fermentative hydrogen-producing strain H53214 was isolated from a deep-sea hydrothermal vent environment, and identified as Caloranaerobacter azorensis based on bacterial 16S rRNA gene analysis. The optimum culture condition for hydrogen production by the bacterium, designated C. azorensis H53214, was investigated by the response surface methodology (RSM). Eight variables including the concentration of NaCl, glucose, yeast, tryptone, FeSO4 and MgSO4, initial pH and incubation temperature were screened based on the Plackett-Burman design. The results showed that initial pH, tryptone and yeast were significant variables, which were further optimized using the steepest ascent method and Box-Behnken design. The optimal culture conditions for hydrogen production were an initial pH of 7.7, 8.3 g L-1 tryptone and 7.9 g L-1 yeast. Under these conditions, the maximum cumulative hydrogen volume, hydrogen yield and maximum H2 production rate were 1.58 L H2 L-1 medium, 1.46 mol H2 mol-1 glucose and 25.7 mmol H2 g -1 cell dry weight (CDW) h-1, respectively. By comparison analysis, strain H53214 was superior to the most thermophilic hydrogen producers because of the high hydrogen production rate. In addition, the isolation of C. azorensis H53214 indicated the deep-sea hydrothermal environment might be a potential source for fermentative hydrogen-producing thermophiles. © 2014, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved. Source


Wang W.,State Key Laboratory Breeding Base of Marine Genetic Resources | Wang W.,State Oceanic Administration | Wang W.,Key Laboratory of Marine Genetic Resources of Fujian Province | Shao Z.,State Key Laboratory Breeding Base of Marine Genetic Resources | And 2 more authors.
Frontiers in Microbiology | Year: 2013

Alkanes are major constituents of crude oil. They are also present at low concentrations in diverse non-contaminated because many living organisms produce them as chemo-attractants or as protecting agents against water loss. Alkane degradation is a widespread phenomenon in nature. The numerous microorganisms, both prokaryotic and eukaryotic, capable of utilizing alkanes as a carbon and energy source, have been isolated and characterized. This review summarizes the current knowledge of how bacteria metabolize alkanes aerobically, with a particular emphasis on the oxidation of long-chain alkanes, including factors that are responsible for chemotaxis to alkanes, transport across cell membrane of alkanes, the regulation of alkane degradation gene and initial oxidation. © 2013 Wang and Shao. Source


Cao J.,Harbin Institute of Technology | Cao J.,State Key Laboratory Breeding Base of Marine Genetic Resources | Cao J.,Third Institute of Oceanography | Cao J.,Key Laboratory of Marine Genetic Resources of Fujian Province | And 10 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2014

A taxonomic study was carried out on strain GCS-AE-31T, which was isolated from a phenol-degrading consortium, enriched from coking wastewater activated sludge of the Beijing Shougang Company Limited during the screening of phenol-degrading bacteria. Cells of strain GCS-AE-31T were Gram-stain-negative, short rods, motile by gliding, oxidase- and catalase-positive. Growth was observed at salinities of 0-3% and at temperatures of 10-37 °C. On the basis of 16S rRNA gene sequence similarity, strain GCS-AE-31Twas most closely related to Pedobacter saltans LMG 10337T (96.17%), but it showed low similarity to all other species of the genus Pedobacter (89.28-92.45%). It also showed low 16S rRNA gene similarity to all other species of the family Sphingobacteriaceae (87.25-92.45 %) examined. The dominant fatty acids were iso-C15: 0, summed feature 3 (C16:1ω7c/C16:1ω6c), anteiso-C15:0and iso-C17:0 3-OH. The menaquinones were MK-7 (95.5%) and MK-6 (4.5%). The polar lipids were phosphatidylethanolamine, three aminolipids and three unknown phospholipids. Sphingolipid was present. The G+C content of the chromosomal DNA was 36.2 mol%. According to its phylogenetic position and phenotypic traits, the novel strain could not be assigned to the genus Pedobacter; it should be classified as representing a novel species of a novel genus in the family Sphingobacteriaceae, for which the name Pseudopedobacter beijingensis gen. nov., sp. nov. is proposed (type strain GCS-AE-31T = MCCC 1A01299T = CGMCC 1.12329T = LMG 27180T). The misclassified species Pedobacter saltans is transferred to the novel genus as Pseudopedobacter saltans comb. nov. (type strain LMG 10337T = MCCC 1A06472T = DSM 12145T = CCUG 39354T = CIP 105500T = JCM 21818T = NBRC 100064T). © 2014 IUMS. Source


Zeng X.,Third Institute of Oceanography SOA | Zeng X.,Key Laboratory of Marine Genetic Resources of Fujian Province | Zhang Z.,Third Institute of Oceanography SOA | Zhang Z.,Key Laboratory of Marine Genetic Resources of Fujian Province | And 6 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2015

A thermophilic, anaerobic, iron-reducing bacterium (strain DY22619T) was isolated from a sulfide sample collected from an East Pacific Ocean hydrothermal field at a depth of 2901 m. Cells were Gram-stain-negative, motile rods (2-10 mm in length, 0.5 mm in width) with multiple peritrichous flagella. The strain grew at 40-70 ºC inclusive (optimum 60 ºC), at pH 4.5-8.5 inclusive (optimum pH 7.0) and with sea salts concentrations of 1-10% (w/v) (optimum 3% sea salts) and NaCl concentrations of 1.5-5.0% (w/v) (optimum 2.5% NaCl). Under optimal growth conditions, the generation time was around 55 min. The isolate was an obligate chemoorganoheterotroph, utilizing complex organic compounds, amino acids, carbohydrates and organic acids including peptone, tryptone, beef extract, yeast extract, alanine, glutamate, methionine, threonine, fructose, mannose, galactose, glucose, palatinose, rhamnose, turanose, gentiobiose, xylose, sorbose, pyruvate, tartaric acid, a-ketobutyric acid, a-ketovaleric acid, galacturonic acid and glucosaminic acid. Strain DY22619T was strictly anaerobic and facultatively dependent on various forms of Fe(III) as an electron acceptor: insoluble forms and soluble forms. It did not reduce sulfite, sulfate, thiosulfate or nitrate. The genomic DNA G+C content was 29.0 mol%. Phylogenetic 16S rRNA gene sequence analyses revealed that the closest relative of strain DY22619T was Caloranaerobacter azorensis MV1087T, sharing 97.41% 16S rRNA gene sequence similarity. On the basis of physiological distinctness and phylogenetic distance, the isolate is considered to represent a novel species of the genus Caloranaerobacter, for which the name Caloranaerobacter http://dx.doi.org/10.1601/nm.4081ferrireducens sp. nov. is proposed. The type strain is DY22619T (=JCM 19467T=DSM 27799T=MCCC1A06455T). © 2015, IUMS. Source

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