Key Laboratory of Marine Fishery Molecular Biology

Liaoning, China

Key Laboratory of Marine Fishery Molecular Biology

Liaoning, China
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Gao X.,Liaoning Ocean and Fisheries Science Research Institute | Gao X.,Key Laboratory of Marine Fishery Molecular Biology | Han J.,Liaoning Ocean and Fisheries Science Research Institute | Han J.,Key Laboratory of Marine Fishery Molecular Biology | And 5 more authors.
Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics | Year: 2012

Next-generation sequencing provides a powerful new approach for developing functional genomic tools for non-model species. This study aims to investigate the spotted seal (Phoca largha) transcriptome by the approach of Illumina paired-end sequencing technology. We obtained a total of 52,146,394 reads for the mixed tissues of liver and spleen from spotted seal. The de novo assemblies yielded 354,014 contigs and 178,466 unigenes. In addition, bioinformatics analysis revealed a total of 4425 simple sequence repeats (SSRs). Fifty SSRs were randomly selected to validate amplification and determine the degree of polymorphism in the genomic DNA pools. Thirty-five primer pairs successfully amplified expected DNA fragments and detected significant polymorphism among 28 spotted seal individuals. These results contribute to the understanding of the genetic makeup of spotted seal transcriptome and provide useful information for functional genomic research in this species. © 2012 Elsevier Inc. All rights reserved.


Shan Z.,Liaoning Normal University | Li H.,Key Laboratory of Marine Fishery Molecular Biology | Li H.,Liaoning Ocean and Fisheries Science Research Institute | Bao X.,Key Laboratory of Marine Fishery Molecular Biology | And 14 more authors.
Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology | Year: 2011

Glutathione peroxidase (GPx) is an antioxidant enzyme that protects cells from oxidative damage in the innate immune responses against bacterial infections. GPx is also involved in immune defenses. In this study, we report cloning and characterization of a GPx (designated as MyGPx) coding sequences and promoter from Japanese scallop, Mizuhopecten yessoensis. The full-length 1081 nt MyGPx mRNA contained a 28 nt 5' untranslated region (UTR), a 603 nt open reading frame and a 450 nt 3' UTR containing a polyadenylation signal (AATAAA). Multiple sequence alignment revealed that amino acids essential to enzymatic function of MyGPx proteins were highly conserved. A 1628 nt 5'-flanking sequence of MyGPx was identified by genome walking. Here, several potential transcription factor binding sites were detected in the putative promoter region, and nine single nucleotide polymorphisms (SNPs) were found in the 5' sequence flanking the promoter region. Quantitative Real time PCR (qRT-PCR) was employed to measure GPx mRNA expression in adult tissues and monitor mRNA expression patterns during embryonic development and following stimulation by the bacteria Vibrillo anguillarum. Collectively, the results suggest that MyGPx fulfills an important function during M. yessoensis development and may be an important immune effector in adult molluscs. © 2011 Elsevier Inc.


Gao X.,Liaoning Ocean and Fisheries Science Research Institute | Gao X.,Key Laboratory of Marine Fishery Molecular Biology | Han J.,Liaoning Ocean and Fisheries Science Research Institute | Han J.,Key Laboratory of Marine Fishery Molecular Biology | And 5 more authors.
Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics | Year: 2013

Spotted seal (Phoca largha) is categorized as a critically endangered species in China. The aim of this study was to investigate spotted seal transcriptome by the approach of Illumina paired-end sequencing technology. We obtained a total of 52,146,394 reads for the mixed tissues of liver and spleen from the spotted seal. The de novo assemblies yielded 354,014 contigs and 178,466 unigenes. In the transcriptome, 193 unigenes were assigned to defense mechanisms. Three unigenes encoded MHC class I and 17 unigenes encoded MHC class II. In addition, bioinformatics analysis revealed a total of 4425 simple sequence repeats (SSRs). Fifty SSRs were randomly selected to validate amplification and determine the degree of polymorphism in the genomic DNA pools. Thirty-five primer pairs successfully amplified the expected DNA fragments and detected significant polymorphism among 28 spotted seal individuals. These results would contribute to the understanding of the genetic makeup of spotted seal transcriptome and provide useful information for functional genomic research in this species. © 2013 Elsevier Inc.

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