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Liu Y.,Shanghai Ocean University | Wang J.Y.,Key laboratory of Marine Ecological Restoration Shandong Marine Resource and Environment Research Institute Yantai China | Li B.S.,Key laboratory of Marine Ecological Restoration Shandong Marine Resource and Environment Research Institute Yantai China | Qiao H.J.,Key laboratory of Marine Ecological Restoration Shandong Marine Resource and Environment Research Institute Yantai China | And 3 more authors.
Aquaculture Nutrition | Year: 2017

An 8-week feeding trial was conducted to estimate the optimum dietary manganese (Mn) requirement for juvenile hybrid grouper, Epinephelus lanceolatus × E. fuscoguttatus. The basal diet was formulated to contain 520 g/kg crude protein from casein and fishmeal. Manganese methionine was added to the basal diet at 0 (control group), 2.5, 5, 10, 20 and 40 mg Mn/kg diet providing 7.48, 10.34, 13.76, 19.72, 31.00 and 53.91 mg Mn/kg diet, respectively. Each diet was randomly fed to triplicate groups of juveniles, and each tank was stocked with 20 fish (initial weight, 60.06 ± 0.68 g). The manganese content in rearing water was monitored and kept below 0.01 mg/L. Results showed that the weight gain ratio (WGR), protein efficiency ratio (PER), specific growth rate (SGR), Mn contents in whole body, liver and vertebra, and activities of hepatic Mn superoxide dismutase (Mn-SOD), total SOD (T-SOD) and glutathione peroxidase (GSH-PX) were significantly improved by dietary Mn supplementation (p < .05). However, dietary Mn did not affect arginase (DArg) activity. The highest feed conversion ratio (FCR) was observed in fish fed the basal diet (p < .05). No significant differences were found on the Cu and Zn contents in whole body by supplementing dietary Mn. Supplemented Mn in diets had significantly effect on liver and vertebral trace element deposition (p < .05). Fish fed the basal diet had the highest Fe and Zn contents in vertebra (p < .05). There were no significant differences on hepatic pyruvate decarboxylase (PDC) activity with supplemented Mn levels below 13.76 mg/kg. As biomarker of oxidative stress, malondialdehyde (MDA) content in liver was significantly higher in fish fed the basal diet (p < .05). Using the broken-line models based on SGR, dietary Mn requirement of the juvenile hybrid grouper was estimated to be 12.70 mg/kg diet. © 2017 John Wiley & Sons Ltd.


Wang J.,Key Laboratory of Marine Ecological Restoration Shandong Marine Resource and Environment Research Institute Yantai China | Zhang D.,Key Laboratory of Marine Ecological Restoration Shandong Marine Resource and Environment Research Institute Yantai China | Sun Y.,Key Laboratory of Marine Ecological Restoration Shandong Marine Resource and Environment Research Institute Yantai China | Wang S.,Key Laboratory of Marine Ecological Restoration Shandong Marine Resource and Environment Research Institute Yantai China | And 3 more authors.
Aquaculture Research | Year: 2014

An 8-week feeding trial was conducted to evaluate the effects of a dairy-yeast prebiotic (Grobiotic®-A) on growth performance, body composition, antioxidant capacity and immune functions of juvenile starry flounder, Platichtahys stellatus. Three triplicates of starry flounder (average initial weight of 15.05 ± 0.04 g) were fed one of six diets formulated to supplement dairy-yeast prebiotic at 0%, 0.4%, 0.8%, 1.2%, 1.6% or 2.0% respectively. Results showed that weight gain, daily feed intake, protein efficiency rate and condition factor increased significantly (P < 0.05) whereas feed conversion ratio decreased significantly with the increasing level of the dairy-yeast prebiotic supplement. Crude lipid content in dorsal muscle and liver decreased significantly (P < 0.05). No significant differences occurred in moisture and crude protein content of dorsal muscle and liver with the increasing level of the dairy-yeast prebiotic supplement. Compared with fish feed the basal diet, the activity of catalase and total antioxidant capability in serum and liver, as well as total superoxide dismutase in serum all significantly increased with high level of the prebiotic, while the malondialdehyde content in serum and liver decreased significantly. In serum, the activity of alkaline phosphatase, lysozyme, complement and the content of nitric oxide were significantly increased at higher level of prebiotic supplementation while no significant differences were found in total protein, albumin, globulin, albumin to globulin ratio, haemoglobin, acid phosphatase and myeloperoxidase. Based on weight gain response using the quadratic regression, combine the antioxidant and immune indices, the optimum dietary dairy-yeast prebiotic level for juvenile starry flounder was estimated to be 1.33% under these experimental conditions. © 2014 John Wiley & Sons Ltd.

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