Key Laboratory of Integrated Pest Management for Fujian Taiwan Crops

Fuzhou, China

Key Laboratory of Integrated Pest Management for Fujian Taiwan Crops

Fuzhou, China
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Chen Y.,Fujian Agriculture and forestry University | Chen Y.,Key Laboratory of Integrated Pest Management for Fujian Taiwan Crops | Vasseur L.,Fujian Agriculture and forestry University | Vasseur L.,Key Laboratory of Integrated Pest Management for Fujian Taiwan Crops | And 3 more authors.
Climatic Change | Year: 2017

Oracella acuta is a significant invasive insect pest in China, which has already caused severe damage to host pines. It is expected that this pest may invade other regions of Asia. This research explores the projected effects of climate change on the future distribution of O. acuta in Asia. To anticipate threats and prioritize management strategies to control O. acuta, we examined the potential distribution of O. acuta under current and future climate scenarios based on CLIMEX models. These models were calibrated using the physiological tolerance thresholds for this species, and A1B and A2 scenarios for 2030 and 2070 under a CSIRO-Mk 3.0 Global Climate Model were used to predict future distribution. The results suggest that O. acuta has the ability to establish in most countries of Southern Asia, such as China, Vietnam, Laos, Myanmar, Bhutan, Bangladesh, Philippines, Sri Lanka, and India. Both scenarios showed that the species is predicted to expand its range northwards but retract in the southern edge. The A1B scenario projected a wider spread of O. acuta than the A2 scenario. Despite the uncertainties inherent to climate models and that it was not possible to integrate all parameters into the model and some assumptions had to be made, our results indicate that heat and drought stress may have significant impacts on the species distribution, especially in southern regions of Asia in the future. © 2017, Springer Science+Business Media Dordrecht.


Wang Y.,Fujian Agriculture and forestry University | Xu T.,Fujian Agriculture and forestry University | He W.,Fujian Agriculture and forestry University | Shen X.,Fujian Agriculture and forestry University | And 3 more authors.
Genomics | Year: 2017

Long non-coding RNAs (lncRNAs) are of particular interest because of their contributions to many biological processes. Here, we present the genome-wide identification and characterization of putative lncRNAs in a global insect pest, Plutella xylostella. A total of 8096 lncRNAs were identified and classified into three groups. The average length of exons in lncRNAs was longer than that in coding genes and the GC content was lower than that in mRNAs. Most lncRNAs were flanked by canonical splice sites, similar to mRNAs. Expression profiling identified 114 differentially expressed lncRNAs during the DBM development and found that majority were temporally specific. While the biological functions of lncRNAs remain uncharacterized, many are microRNA precursors or competing endogenous RNAs involved in micro-RNA regulatory pathways. This work provides a valuable resource for further studies on molecular bases for development of DBM and lay the foundation for discovery of lncRNA functions in P. xylostella. © 2017 Elsevier Inc.


Zhao Q.,Fujian Agriculture and forestry University | Zhao Q.,Key Laboratory of Integrated Pest Management for Fujian Taiwan Crops | Ma D.,Fujian Agriculture and forestry University | Ma D.,Key Laboratory of Integrated Pest Management for Fujian Taiwan Crops | And 4 more authors.
BMC Evolutionary Biology | Year: 2017

Background: Structural variation among genomes is now viewed to be as important as single nucleoid polymorphisms in influencing the phenotype and evolution of a species. Segmental duplication (SD) is defined as segments of DNA with homologous sequence. Results: Here, we performed a systematic analysis of segmental duplications (SDs) among five lepidopteran reference genomes (Plutella xylostella, Danaus plexippus, Bombyx mori, Manduca sexta and Heliconius melpomene) to understand their potential impact on the evolution of these species. We find that the SDs content differed substantially among species, ranging from 1.2% of the genome in B. mori to 15.2% in H. melpomene. Most SDs formed very high identity (similarity higher than 90%) blocks but had very few large blocks. Comparative analysis showed that most of the SDs arose after the divergence of each linage and we found that P. xylostella and H. melpomene showed more duplications than other species, suggesting they might be able to tolerate extensive levels of variation in their genomes. Conserved ancestral and species specific SD events were assessed, revealing multiple examples of the gain, loss or maintenance of SDs over time. SDs content analysis showed that most of the genes embedded in SDs regions belonged to species-specific SDs ("Unique" SDs). Functional analysis of these genes suggested their potential roles in the lineage-specific evolution. SDs and flanking regions often contained transposable elements (TEs) and this association suggested some involvement in SDs formation. Further studies on comparison of gene expression level between SDs and non-SDs showed that the expression level of genes embedded in SDs was significantly lower, suggesting that structure changes in the genomes are involved in gene expression differences in species. Conclusions: The results showed that most of the SDs were "unique SDs", which originated after species formation. Functional analysis suggested that SDs might play different roles in different species. Our results provide a valuable resource beyond the genetic mutation to explore the genome structure for future Lepidoptera research. © 2017 The Author(s).


Xu Y.,Fujian Agriculture and forestry University | Fan Q.-H.,Plant Health and Environment Laboratory | Zhang F.-P.,Fujian Agriculture and forestry University | Huang J.,Fujian Agriculture and forestry University | And 2 more authors.
Systematic and Applied Acarology | Year: 2017

The ontogenetic stages of a tenuipapid mite, Aegyptobia exarata Livchitz & Mitrofanov, 1967, recently discovered from Artemisia sp. (Asteraceae) in Shanxi province of China are redescribed with detailed illustrations. The ontogenetic changes in idiosomal and leg chaetotaxy are discussed. © Systematic & Applied Acarology Society.


Yu L.,Fujian Agriculture and forestry University | Yu L.,Key Laboratory of Integrated Pest Management for Fujian Taiwan Crops | Tang W.,Fujian Agriculture and forestry University | He W.,Fujian Agriculture and forestry University | And 15 more authors.
Scientific Reports | Year: 2015

Cytochrome P450 monooxygenases are present in almost all organisms and can play vital roles in hormone regulation, metabolism of xenobiotics and in biosynthesis or inactivation of endogenous compounds. In the present study, a genome-wide approach was used to identify and analyze the P450 gene family of diamondback moth, Plutella xylostella, a destructive worldwide pest of cruciferous crops. We identified 85 putative cytochrome P450 genes from the P. xylostella genome, including 84 functional genes and 1 pseudogene. These genes were classified into 26 families and 52 subfamilies. A phylogenetic tree constructed with three additional insect species shows extensive gene expansions of P. xylostella P450 genes from clans 3 and 4. Gene expression of cytochrome P450s was quantified across multiple developmental stages (egg, larva, pupa and adult) and tissues (head and midgut) using P. xylostella strains susceptible or resistant to insecticides chlorpyrifos and fiprinol. Expression of the lepidopteran specific CYP367s predominantly occurred in head tissue suggesting a role in either olfaction or detoxification. CYP340s with abundant transposable elements and relatively high expression in the midgut probably contribute to the detoxification of insecticides or plant toxins in P. xylostella. This study will facilitate future functional studies of the P. xylostella P450s in detoxification.


Gurr G.M.,Fujian Agriculture and forestry University | Gurr G.M.,Key Laboratory of Integrated Pest Management for Fujian Taiwan Crops | Gurr G.M.,Charles Sturt University | You M.,Fujian Agriculture and forestry University | You M.,Key Laboratory of Integrated Pest Management for Fujian Taiwan Crops
Frontiers in Plant Science | Year: 2016

Biological control has long been considered a potential alternative to pesticidal strategies for pest management but its impact and level of use globally remain modest and inconsistent. A rapidly expanding range of molecular - particularly DNA-related-techniques is currently revolutionizing many life sciences. This review identifies a series of constraints on the development and uptake of conservation biological control and considers the contemporary and likely future influence of molecular methods on these constraints. Molecular approaches are now often used to complement morphological taxonomic methods for the identification and study of biological control agents including microbes. A succession of molecular techniques has been applied to ‘who eats whom’ questions in food-web ecology. Polymerase chain reaction (PCR) approaches have largely superseded immunological approaches such as enzyme-linked immunosorbent assay (ELISA) and now - in turn - are being overtaken by next generation sequencing (NGS)-based approaches that offer unparalleled power at a rapidly diminishing cost. There is scope also to use molecular techniques to manipulate biological control agents, which will be accelerated with the advent of gene editing tools, the CRISPR/Cas9 system in particular. Gene editing tools also offer unparalleled power to both elucidate and manipulate plant defense mechanisms including those that involve natural enemy attraction to attacked plants. Rapid advances in technology will allow the development of still more novel pest management options for which uptake is likely to be limited chiefly by regulatory hurdles. © 2016 Gurr and You.


Hu G.P.,Fujian Agriculture and forestry University | Hu G.P.,Key Laboratory of Integrated Pest Management for Fujian Taiwan Crops | Zhao Y.,Fujian Agriculture and forestry University | Zhao Y.,Key Laboratory of Integrated Pest Management for Fujian Taiwan Crops | And 9 more authors.
Research in Microbiology | Year: 2014

Strain FLQ-11-1, isolated from sewage sludge, was able to degrade cyfluthrin and was identified as Lysinibacillus sphaericus based on its morphology, 16S rRNA sequence and fatty acid methyl ester (FAME) analyses. This strain could use cyfluthrin as its carbon or nitrogen source. Response surface methodology (RSM) analysis showed that the optimum conditions for degradation were at pH 7.0 and 35°C, using an inoculum amount with an OD600nm value of 1.6. Under these conditions, approximately 80.4% of cyfluthrin (50mgl-1) was degraded within five days (d) of incubation. Four metabolic compounds were detected during cyfluthrin degradation and identified as methyl-3-(2,2-dichlorovinyl)-2,2-dimethyl-(1-cyclopropane)-carboxylate, 4-fluoro-3-phenoxy-benzoic acid methyl ester, methyl-3-phenoxybenzoate, 3-phenoxy-benzaldehyde by gas chromatography-mass spectrometry (GC-MS) and tandem mass spectrum (MS/MS) analysis and no cyfluthrin was detected after seven days of incubation. A possible degradation pathway was proposed, and our data showed that cyfluthrin could be efficiently degraded by FLQ-11-1, indicating that this strain could potentially be used to eliminate the contamination of pyrethroid herbicides. © 2013 Institut Pasteur.


Zhang S.L.,Fujian Agriculture and forestry University | Liu G.K.,Key Laboratory of Integrated Pest Management for Fujian Taiwan Crops | Liu G.K.,Ghent University | Janssen T.,Ghent University | And 5 more authors.
Plant Pathology | Year: 2014

Surveys conducted in peanut production areas of China revealed peanut pod rot in several fields in Shandong and Hebei Provinces, China. A large quantity of an unknown stem nematode was isolated from the hulls and seeds of peanuts, herein described as Ditylenchus arachis n. sp. The new species is characterized by a combination of the following features: lateral lip sectors distinctly projected, stylet delicate, 8·4-10 μm in length, six lines in the lateral field, tail elongate-conoid, bursa covering about 68-86% of tail length. Pathogenicity tests showed that D. arachis n. sp. could infect peanut (Arachis hypogaea), but not sweet potato (Ipomoea batatas) or potato (Solanum tuberosum). Morphologically, D. arachis n. sp. appears closest to D. africanus, D. myceliophagus and D. destructor, but can be differentiated based upon a combination of morphological characteristics, host preference and molecular sequence data. The results of the phylogenetic analysis, based on 18S rDNA, the D2-D3 expansion region of 28S rDNA, and the ITS1-5·8S-ITS2 region, confirmed its status as a new species. A sister relationship with D. destructor was appointed, rather than with its ecologically very similar congener D. africanus. © 2013 British Society for Plant Pathology.


Ding X.,Fujian Agriculture and forestry University | Ouyang M.-A.,Fujian Agriculture and forestry University | Liu X.,Key Laboratory of Integrated Pest Management for Fujian Taiwan Crops | Wang R.-Z.,Fujian Agriculture and forestry University
Journal of Chemistry | Year: 2013

Ginkgo biloba is a traditional Chinese medicinal plant which has potent insecticidal activity against brown planthopper. The MeOH extract was tested in the acetylcholinesterase (AChE) inhibitory assay with IC50 values of 252.1 g/mL. Two ginkgolides and thirteen flavonoids were isolated from the leaves of Ginkgo biloba. Their structures were established on the basis of spectroscopic data interpretation. It revealed that the 13 isolated flavonoids were found to inhibit AChE with IC50 values ranging from 57.8 to 133.1 g/mL in the inhibitory assay. AChE was inhibited dose dependently by all tested flavonoids, and compound 6 displayed the highest inhibitory effect against AChE with IC50 values of 57.8 g/mL. © 2013 Xiao Ding et al.


Hou Y.,Key Laboratory of Insect Ecology in Fujian | Hou Y.,Key Laboratory of Integrated Pest Management for Fujian Taiwan Crops | Miao Y.,Key Laboratory of Insect Ecology in Fujian | Miao Y.,Key Laboratory of Integrated Pest Management for Fujian Taiwan Crops | And 2 more authors.
Journal of Economic Entomology | Year: 2014

In southeastern China, the invasion of the nipa palm hispid Octodonta nipae (Maulik) (Coleoptera: Chrysomelidae) results in devastating damage to palms. Host plants play an important role in the population increases and outbreaks of O. nipae. O. nipae could not complete its development on the Majestic palm (Ravenea rivularis Jumelle & Perrier), and females did not lay eggs on Chinese fan palm (Livistona chinensis R. Brown). However, this insect species both completed development and laid eggs on Chinese windmill palm (Trachycarpus fortunei (Hooker) H. Wendland), Canary Island date palm (Phoenix canariensis Chabaud), and pygmy date palm (Phoenix roebelenii O' Brien). The demographic characteristics of O. nipae reared on Chinese windmill palm, Canary Island date palm, and pygmy date palm were compared with an age-stage, two-sex life table. In this study, the developmental periods from egg to adult varied from 42.1 d on Chinese windmill palm to 49.8 d on pygmy date palm. The survivorship from egg to adult on Chinese windmill palm, Canary Island date palm, and pygmy date palm was 77.5, 79.4, and 66.7%, respectively. Although the adult longevity and the mean fecundity for individuals reared on Chinese windmill palm, Canary Island date palm, and pygmy date palm were not significantly different, there were significant differences in the intrinsic rate of increase, the finite rate, and the mean generation time among palm species, and the values of intrinsic rate of increase and finite rate were higher for populations reared on Chinese windmill palm and Canary Island date palm (0.0313 and 1.0318 d-1 and 0.0278 and 1.0282 d-1, respectively) and lower for populations reared on pygmy date palm (0.0192 and 1.0194 d-1). However, mean generation time was shorter on Chinese windmill palm (124.11 d) and Canary Island date palm (129.62 d) and longer on pygmy date palm (166.03 d). Our study indicated that different hosts affected life parameters of O. nipae, with the most preferred hosts being the Chinese windmill palm and Canary Island date palm. These results may be useful for the design of culture management strategies for O. nipae. © 2014 Entomological Society of America.

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