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Chen Y.,Fujian Agriculture and forestry University | Chen Y.,Key Laboratory of Integrated Pest Management for Fujian Taiwan Crops | Vasseur L.,Fujian Agriculture and forestry University | Vasseur L.,Key Laboratory of Integrated Pest Management for Fujian Taiwan Crops | And 3 more authors.
Climatic Change | Year: 2017

Oracella acuta is a significant invasive insect pest in China, which has already caused severe damage to host pines. It is expected that this pest may invade other regions of Asia. This research explores the projected effects of climate change on the future distribution of O. acuta in Asia. To anticipate threats and prioritize management strategies to control O. acuta, we examined the potential distribution of O. acuta under current and future climate scenarios based on CLIMEX models. These models were calibrated using the physiological tolerance thresholds for this species, and A1B and A2 scenarios for 2030 and 2070 under a CSIRO-Mk 3.0 Global Climate Model were used to predict future distribution. The results suggest that O. acuta has the ability to establish in most countries of Southern Asia, such as China, Vietnam, Laos, Myanmar, Bhutan, Bangladesh, Philippines, Sri Lanka, and India. Both scenarios showed that the species is predicted to expand its range northwards but retract in the southern edge. The A1B scenario projected a wider spread of O. acuta than the A2 scenario. Despite the uncertainties inherent to climate models and that it was not possible to integrate all parameters into the model and some assumptions had to be made, our results indicate that heat and drought stress may have significant impacts on the species distribution, especially in southern regions of Asia in the future. © 2017, Springer Science+Business Media Dordrecht.


Yu L.,Fujian Agriculture and forestry University | Yu L.,Key Laboratory of Integrated Pest Management for Fujian Taiwan Crops | Tang W.,Fujian Agriculture and forestry University | He W.,Fujian Agriculture and forestry University | And 15 more authors.
Scientific Reports | Year: 2015

Cytochrome P450 monooxygenases are present in almost all organisms and can play vital roles in hormone regulation, metabolism of xenobiotics and in biosynthesis or inactivation of endogenous compounds. In the present study, a genome-wide approach was used to identify and analyze the P450 gene family of diamondback moth, Plutella xylostella, a destructive worldwide pest of cruciferous crops. We identified 85 putative cytochrome P450 genes from the P. xylostella genome, including 84 functional genes and 1 pseudogene. These genes were classified into 26 families and 52 subfamilies. A phylogenetic tree constructed with three additional insect species shows extensive gene expansions of P. xylostella P450 genes from clans 3 and 4. Gene expression of cytochrome P450s was quantified across multiple developmental stages (egg, larva, pupa and adult) and tissues (head and midgut) using P. xylostella strains susceptible or resistant to insecticides chlorpyrifos and fiprinol. Expression of the lepidopteran specific CYP367s predominantly occurred in head tissue suggesting a role in either olfaction or detoxification. CYP340s with abundant transposable elements and relatively high expression in the midgut probably contribute to the detoxification of insecticides or plant toxins in P. xylostella. This study will facilitate future functional studies of the P. xylostella P450s in detoxification.


You M.,Fujian Agriculture and forestry University | You M.,Key Laboratory of Integrated Pest Management for Fujian Taiwan Crops | Yue Z.,BGI Shenzhen | He W.,Fujian Agriculture and forestry University | And 81 more authors.
Nature Genetics | Year: 2013

How an insect evolves to become a successful herbivore is of profound biological and practical importance. Herbivores are often adapted to feed on a specific group of evolutionarily and biochemically related host plants, but the genetic and molecular bases for adaptation to plant defense compounds remain poorly understood. We report the first whole-genome sequence of a basal lepidopteran species, Plutella xylostella, which contains 18,071 protein-coding and 1,412 unique genes with an expansion of gene families associated with perception and the detoxification of plant defense compounds. A recent expansion of retrotransposons near detoxification-related genes and a wider system used in the metabolism of plant defense compounds are shown to also be involved in the development of insecticide resistance. This work shows the genetic and molecular bases for the evolutionary success of this worldwide herbivore and offers wider insights into insect adaptation to plant feeding, as well as opening avenues for more sustainable pest management. © 2013 Nature America, Inc. All rights reserved.


Gurr G.M.,Fujian Agriculture and forestry University | Gurr G.M.,Key Laboratory of Integrated Pest Management for Fujian Taiwan Crops | Gurr G.M.,Charles Sturt University | You M.,Fujian Agriculture and forestry University | You M.,Key Laboratory of Integrated Pest Management for Fujian Taiwan Crops
Frontiers in Plant Science | Year: 2016

Biological control has long been considered a potential alternative to pesticidal strategies for pest management but its impact and level of use globally remain modest and inconsistent. A rapidly expanding range of molecular - particularly DNA-related-techniques is currently revolutionizing many life sciences. This review identifies a series of constraints on the development and uptake of conservation biological control and considers the contemporary and likely future influence of molecular methods on these constraints. Molecular approaches are now often used to complement morphological taxonomic methods for the identification and study of biological control agents including microbes. A succession of molecular techniques has been applied to ‘who eats whom’ questions in food-web ecology. Polymerase chain reaction (PCR) approaches have largely superseded immunological approaches such as enzyme-linked immunosorbent assay (ELISA) and now - in turn - are being overtaken by next generation sequencing (NGS)-based approaches that offer unparalleled power at a rapidly diminishing cost. There is scope also to use molecular techniques to manipulate biological control agents, which will be accelerated with the advent of gene editing tools, the CRISPR/Cas9 system in particular. Gene editing tools also offer unparalleled power to both elucidate and manipulate plant defense mechanisms including those that involve natural enemy attraction to attacked plants. Rapid advances in technology will allow the development of still more novel pest management options for which uptake is likely to be limited chiefly by regulatory hurdles. © 2016 Gurr and You.


Hu G.P.,Fujian Agriculture and forestry University | Hu G.P.,Key Laboratory of Integrated Pest Management for Fujian Taiwan Crops | Zhao Y.,Fujian Agriculture and forestry University | Zhao Y.,Key Laboratory of Integrated Pest Management for Fujian Taiwan Crops | And 9 more authors.
Research in Microbiology | Year: 2014

Strain FLQ-11-1, isolated from sewage sludge, was able to degrade cyfluthrin and was identified as Lysinibacillus sphaericus based on its morphology, 16S rRNA sequence and fatty acid methyl ester (FAME) analyses. This strain could use cyfluthrin as its carbon or nitrogen source. Response surface methodology (RSM) analysis showed that the optimum conditions for degradation were at pH 7.0 and 35°C, using an inoculum amount with an OD600nm value of 1.6. Under these conditions, approximately 80.4% of cyfluthrin (50mgl-1) was degraded within five days (d) of incubation. Four metabolic compounds were detected during cyfluthrin degradation and identified as methyl-3-(2,2-dichlorovinyl)-2,2-dimethyl-(1-cyclopropane)-carboxylate, 4-fluoro-3-phenoxy-benzoic acid methyl ester, methyl-3-phenoxybenzoate, 3-phenoxy-benzaldehyde by gas chromatography-mass spectrometry (GC-MS) and tandem mass spectrum (MS/MS) analysis and no cyfluthrin was detected after seven days of incubation. A possible degradation pathway was proposed, and our data showed that cyfluthrin could be efficiently degraded by FLQ-11-1, indicating that this strain could potentially be used to eliminate the contamination of pyrethroid herbicides. © 2013 Institut Pasteur.


Zhang S.L.,Fujian Agriculture and forestry University | Liu G.K.,Key Laboratory of Integrated Pest Management for Fujian Taiwan Crops | Liu G.K.,Ghent University | Janssen T.,Ghent University | And 5 more authors.
Plant Pathology | Year: 2014

Surveys conducted in peanut production areas of China revealed peanut pod rot in several fields in Shandong and Hebei Provinces, China. A large quantity of an unknown stem nematode was isolated from the hulls and seeds of peanuts, herein described as Ditylenchus arachis n. sp. The new species is characterized by a combination of the following features: lateral lip sectors distinctly projected, stylet delicate, 8·4-10 μm in length, six lines in the lateral field, tail elongate-conoid, bursa covering about 68-86% of tail length. Pathogenicity tests showed that D. arachis n. sp. could infect peanut (Arachis hypogaea), but not sweet potato (Ipomoea batatas) or potato (Solanum tuberosum). Morphologically, D. arachis n. sp. appears closest to D. africanus, D. myceliophagus and D. destructor, but can be differentiated based upon a combination of morphological characteristics, host preference and molecular sequence data. The results of the phylogenetic analysis, based on 18S rDNA, the D2-D3 expansion region of 28S rDNA, and the ITS1-5·8S-ITS2 region, confirmed its status as a new species. A sister relationship with D. destructor was appointed, rather than with its ecologically very similar congener D. africanus. © 2013 British Society for Plant Pathology.


Zhuang H.M.,Fujian Agriculture and forestry University | Zhuang H.M.,Key Laboratory of Integrated Pest Management for Fujian Taiwan Crops | Li C.W.,Fujian Agriculture and forestry University | Li C.W.,Key Laboratory of Integrated Pest Management for Fujian Taiwan Crops | And 2 more authors.
Molecular Biology Reports | Year: 2014

A full-length acetylcholinesterase (AChE) cDNA sequence (Os-ace2.s) from insecticide-susceptible (S) parasitoid Oomyzus sokolowskii (Hymenoptera: Eulophidae) and a partial cDNA sequence (Os-ace2.r) from insecticide- resistant (R) O. sokolowskii were identified firstly. Both Os-ace2.s (encoding a protein of 639 amino acid residues) and Os-ace2.r (encoding a protein of 530 amino acid residues) contained the typical conserved motifs, including FGESAGdomains, catalytic triad, acyl pocket, three oxy-anino hole, choline binding site, peripheral anionic site, omega loop and conserved aromatic residues. The multiple alignment and Blast results indicated that Os-ace2.s were ace2 member of AChE gene. There were three replacements of the amino acid residues (Glu 115 Leu, Phe 394 Leu, and Lys 424 Arg) between Os-ace2.s and Os-ace2.r. The ace2 of O. sokolowskii was the AChE gene firstly isolated from hymenopteran parasitoid so far. R O. sokolowskii displayed about 15-20-folds resistance ratios to methamidophos and avermectin. The bimolecular rate constant (ki) value in S O. sokolowskii was 3.8-folds for methamidophos and 12.3 for dichlorvos, respectively higher than those in R O. sokolowskii. The results indicated that the insensitive AChE and replacements of the amino acid residues in Os-ace2 might be involved in the resistance to methamidophos in R O. sokolowskii. © 2014, Springer Science+Business Media Dordrecht.


Wang Z.H.,Key Laboratory of Integrated Pest Management for Fujian Taiwan Crops | Wang Z.H.,Fujian Provincial Key Laboratory of Insect Ecology | Wang Z.H.,Fujian Agriculture and forestry University | Huang J.,Key Laboratory of Integrated Pest Management for Fujian Taiwan Crops | And 3 more authors.
Journal of Natural History | Year: 2014

The Chinese species of the genus Coccobius Ratzeburg (Hymenoptera: Aphelinidae) are reviewed. Sixteen species, C. abdominis Huang, C. annulicornis Ratzeburg, C. azumai Tachikawa, C. chaoi Huang, C. curtifuniculatus Huang, C. flaviceps (Girault & Dodd), C. flavicornis Compere & Annecke, C. fulvus (Compere & Annecke), C. furviflagellatus Huang, C. furvus Huang, C. languidus Huang, C. longialatus Huang, C. longifuniculatus Huang, C. maculatus Huang, C. wuyiensis Huang, and one new species, Coccobius albiscutellum Wang and Huang sp.nov., are treated. A key is provided for the recognition of the females of all Chinese species of Coccobius, and notes are given on their use in biological control of scale insect pests. http://www.zoobank.org/urn:lsid:zoobank.org:pub:F510059B-F176-46BA-B7B5-8252B06BE91F. © 2013 © 2013 Taylor & Francis.

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