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Wang H.,Hunan University of Science and Technology | Wang H.,Key Laboratory of Integrated Management of the Pests and Diseases on Horticultural Crops in Hunan Province | Meng J.,Hunan University of Science and Technology | Peng X.,Hunan University of Science and Technology | And 6 more authors.
Plant Molecular Biology

WRKY transcription factors have been implicated in the regulation of transcriptional reprogramming associated with various plant processes but most notably with plant defense responses to pathogens. Here we demonstrate that expression of rice WRKY4 gene (OsWRKY4) was rapidly and strongly induced upon infection of Rhizoctonia solani, the causing agent of rice sheath blight, and exogenous jasmonic acid (JA) and ethylene (ET). OsWRKY4 is localized to the nucleus of plant cells and possesses transcriptional activation ability. Modulation of OsWRKY4 transcript levels by constitutive overexpression increases resistance to the necrotrophic sheath blight fungus, concomitant with elevated expression of JA- and ET-responsive pathogenesis-related (PR) genes such as PR1a, PR1b, PR5 and PR10/PBZ1. Suppression by RNA interference (RNAi), on the other hand, compromises resistance to the fungal pathogen. Yeast one-hybrid assay and transient expression in tobacco cells reveal that OsWRKY4 specifically binds to the promoter regions of PR1b and PR5 which contain W-box (TTGAC[C/T]), or W-box like (TGAC[C/T]) cis-elements. In conclusion, we propose that OsWRKY4 functions as an important positive regulator that is implicated in the defense responses to rice sheath blight via JA/ET-dependent signal pathway. © 2015, Springer Science+Business Media Dordrecht. Source

Cheng F.,Hunan Academy of Agricultural science | Cheng F.,Key Laboratory of Integrated Management of the Pests and Diseases on Horticultural Crops in Hunan Province | Wang J.,Hunan Academy of Agricultural science | Song Z.,Hunan Academy of Agricultural science | And 7 more authors.
Journal of Biotechnology

Bacillus thuringiensis is an important microbial biopesticide for controlling agricultural pests by the production of toxic parasporal crystals proteins. Here,we report the finished annotated genome sequence of B. thuringiensis YC-10,which is highly toxic to nematodes. The complete genome sequence consists of a circular chromosome and nine circular plasmids,which the biggest plasmid harbors six parasporal crystals proteins genes consisting of cry1Aa, cry1Ac, cry1Ia, cry2Aa, cry2Ab and cryB1. The crystals proteins of Cry1Ia and Cry1Aa have high nematicidal activity against Meloidogyne incognita. © 2015 Elsevier B.V. Source

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