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Zhang H.,Nanjing Agricultural University | Zhang H.,Key Laboratory of Integrated | Zhao Q.,Nanjing Agricultural University | Zhao Q.,Key Laboratory of Integrated | And 18 more authors.
Molecular Plant-Microbe Interactions | Year: 2014

The mitogen-activated protein kinase MoOsm1-mediated osmoregulation pathway plays crucial roles in stress responses, asexual and sexual development, and pathogenicity in Magnaporthe oryzae. Utilizing an affinity purification approach, we identified the putative transcriptional activator MoMsn2 as a protein that interacts with MoOsm1 in vivo. Disruption of the MoMSN2 gene resulted in defects in aerial hyphal growth, conidial production, and infection of host plants. Quantitative reverse transcription-polymerase chain reaction analysis showed that the expression of several genes involved in conidiophore formation was reduced in ?Momsn2, suggesting that MoMsn2 might function as a transcriptional regulator of these genes. Subsequently, MoCos1 was identified as one of the MoMsn2 targets through yeast one-hybrid analysis in which MoMsn2 binds to the AGGGG and CCCCT motif of the MoCOS1 promoter region. Phenotypic characterization showed that MoMsn2 was required for appressorium formation and penetration and pathogenicity. Although the ?Momsn2 mutant was tolerant to the cell-wall stressor Calcofluor white, it was sensitive to common osmotic stressors. Further analysis suggests that MoMsn2 is involved in the regulation of the cell-wall biosynthesis pathway. Finally, transcriptome data revealed that MoMsn2 modulates numerous genes participating in conidiation, infection, cell-wall integrity, and stress response. Collectively, our results led to a model in which MoMsn2 mediates a series of downstream genes that control aerial hyphal growth, conidiogenesis, appressorium formation, cell-wall biosynthesis, and infection and that also offer potential targets for the development of new disease management strategies. © 2014 The American Phytopathological Society.

Bo X.,Tangshan College | Zhou P.,Key Laboratory of Integrated | Dai K.,TSIVTC | Wang X.,Tangshan College
Proceedings of 2011 International Conference on Electronic and Mechanical Engineering and Information Technology, EMEIT 2011 | Year: 2011

In view of the blocking problem existing in automatic production line for certain large H-shaped steel at the stage of production, the paper will analyze the cause and put forward optimization method for the motion of stratifying machine. Under the circumstance of no increase of equipment hardware, technical transformation (soft reconstruction) will be made only for the flow procedure of S7-400PLC, which will not only improve the working efficiency, but also resolve the problem of steel blocking thoroughly. © 2011 IEEE.

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