Time filter

Source Type

Han X.-Q.,Peking University | Han X.-Q.,Chinese University of Hong Kong | Li W.-J.,Soochow University of China | Ko C.-H.,Chinese University of Hong Kong | And 4 more authors.
Journal of Asian Natural Products Research | Year: 2013

A protein-bound polysaccharide fraction (JBP-1) was obtained from the fruiting bodies of Cantharellus cibarius. Its chemical composition was studied by the cooperative usage of multiple chemical and spectral methods and characterized to be a fraction with a molecular weight of 4.8 × 10 5 Da and only composed of glucose. Methylation analysis revealed that the sugar residues in JBP-1 are existing as t-, 1,6-, and 1,3,6-linked Glcp sugar residues. The immunocompetence of the fraction was evaluated with the proliferation assay of mouse splenocytes, and the result revealed that JBP-1 could significantly stimulate the proliferation of mouse splenocytes in a dose-dependent manner, with p < 0.001 at the concentration of 100 μg/ml and 30 μg/ml, p < 0.05 at 10 μg/ml. These results give us a primary scientific evidence to further explore the pharmaceutical function of this mushroom. © 2013 Taylor & Francis.

Han X.-Q.,Peking University | Gong F.-Y.,Soochow University of China | Gong F.-Y.,Key Laboratory of Infection and Immunity of Jiangsu Province | Dong H.,Soochow University of China | And 4 more authors.
Glycobiology | Year: 2012

Β-Glucans derived from various sources such as yeast cell walls and medicinal mushrooms are considered as valuable biological response modifiers for their ability to enhance the activity of immune cells, aid in wound healing and help prevent infections. We herein characterize the structure of a novel water-soluble polysaccharide (Zhuling polysaccharide, ZPS) from the fruit bodies of medicinal mushroom Polyporus umbellatus and investigate its immunobiological function. ZPS has a molecular mass of 2.27 × 103 kDa and contains >90 d-glucose as its monosaccharide constituent. On the basis of partial acid hydrolysis, methylation analysis, Fourier transform infrared spectroscopy and nuclear magnetic resonance spectroscopy and the ideal repeating unit of ZPS is established: (1 → 6, 1 → 4)-linked-d-glucopyranosyl backbone, substituted at O-3 position of (1 → 6)-linked-d-glucopyranosyl by (1 → 3)-linked-d-glucopyranosyl branches. ZPS consists of approximately 2930 repeating units, each contains a side chain of no more than three residues in length. Functionally, ZPS is a potent activator of B cells, macrophages and dendritic cells. Depletion of ZPS branches causes a substantial reduction in its ability not only to activate B cells in vitro but also to elicit specific IgM production in vivo. Virtually all healthy human subjects possess high-titer circulating antibodies against ZPS backbone, suggesting that ZPS epitope is shared by environmental antigens capable of eliciting adaptive humoral responses in the population. © 2012 The Author.

Loading Key Laboratory of Infection and Immunity of Jiangsu Province collaborators
Loading Key Laboratory of Infection and Immunity of Jiangsu Province collaborators