Key laboratory of Immunology in Universities of Shandong

Weifang, China

Key laboratory of Immunology in Universities of Shandong

Weifang, China
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Tian J.,Key laboratory of Immunology in Universities of Shandong | Di D.,Key laboratory of Immunology in Universities of Shandong | Wang H.,Key laboratory of Immunology in Universities of Shandong | Gong F.,Key laboratory of Immunology in Universities of Shandong | And 2 more authors.
Chinese Journal of Cancer Biotherapy | Year: 2013

Objective: To study the effects of overexpression of BCSC-1 gene on the proliferation, invasion, adherence and migration abilities of hepatoma carcinoma Bel-7402 cells, and to investigate the possible mechanism. Methods: Bel-7402 cells were transfected by pcDNA3. l/v5-HisB-BCSC-l and pcDNA3. l/v5-HisB respectively as BCSC-1 group and empty vector group, and the wild-type Bel-7402 cells as a wild group. Proliferation, invasion, adherence and migration abilities of the Bel-7402 cells were detected by MTT assay, Transwell assay, adhesion experiment in vitro and scratch test, respectively. Real-time PCR was performed to detect the expression of BCSC-1 and osteopontin (OPN), ICAM-1, PTTG mRNA, which were correlated with the cell growth and adhesion in Bel-7402 cells. Results: After transfected by pcDNA3. l/v5-HisB-BCSC-l, the expression of BCSC-1 mRNA in Bel-7402 cells was significantly raised compared with that of empty vector group and the wild-type group ([10. 58 ± 0. 56] vs [1. 10 ± 0. 22], [1. 00 ± 0. 01]; all P < 0. 01). The Bel-7402 cell line stably overexpressing BCSC-1 was successfully established. Compared with the empty vector group and wild group, the growth rate of el-7402 cells in the S-1 group was obviously slower (72 h: [0.29 ± 0.003] vs [0.34 ±0.014], [0. 35 ±0.013]; all P < 0. 05), the invasion rate ([76.20 ± 1. 85]% vs [93.42 ±3.24]%, [100. 00 ± 1. 05]%; all P < 0. 01) and the adhesion rate ([58. 57 ± 0. 84]% vs [97. 14 ± 0. 84]%, [100. 00 ± 1. 30]%; all P <0. 01) were obviously decreased, the migratory distance was significantly reduced ([116. 60 ± 10. 58 J vs [231. 33 ± 10. 26], [237. 96 ± 11. 58] μm; all P < 0. 01]. The expression of OPN mRNA in the Bel-7402 cells which the BCSC-1 was up-regulated was significantly decreased ([0. 12 ± 0. 06] vs [0. 95 ± 0. 14], [1. 00 ± 0. 08]; all P <0. 01). No significant changes were observed in the expression of ICAM-1 and PTTG mRNA. Conclusion: Overex-pression of BCSC-1 can inhibit the proliferation, invasion, adherence and migration abilities of Bel-7402 cells, which may be related to the decreased expression of OPN.


Lin Z.J.,Key Laboratory of Immunology in Universities of Shandong
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2011

To analyze the effect of recombinant IL-1β on proliferation, migration, and the effect on IFNα induced cell growth inhibtion. The vector pLIVE-mIL-1β was transfected into Hepa1-6 cells mediated by transIT-LT1. Gene expression level of IL-1β was analyzed by RT-PCR and Sandwich ELISA. Cell migration was assessed using wound healing assay. IL-1β significantly stimulated proliferation and migration of Hepa1-6 cells. However, expression of IL-1β significantly down-regulated growth inhibition inducecd by IFNα. The recombinant vector could stably express IL-1β and promote in vitro proliferation, migration, and impair IFNα-induced cell growth inhibition.


PubMed | Key Laboratory of Immunology in Universities of Shandong
Type: Journal Article | Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2011

To analyze the effect of recombinant IL-1 on proliferation, migration, and the effect on IFN induced cell growth inhibtion.The vector pLIVE-mIL-1 was transfected into Hepa1-6 cells mediated by transIT-LT1. Gene expression level of IL-1 was analyzed by RT-PCR and Sandwich ELISA. Cell migration was assessed using wound healing assay.IL-1 significantly stimulated proliferation and migration of Hepa1-6 cells. However, expression of IL-1 significantly down-regulated growth inhibition inducecd by IFN.The recombinant vector could stably express IL-1 and promote in vitro proliferation, migration, and impair IFN-induced cell growth inhibition.

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