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Lin Z.J.,Key laboratory of Immunology in Universities of Shandong
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2011

To analyze the effect of recombinant IL-1β on proliferation, migration, and the effect on IFNα induced cell growth inhibtion. The vector pLIVE-mIL-1β was transfected into Hepa1-6 cells mediated by transIT-LT1. Gene expression level of IL-1β was analyzed by RT-PCR and Sandwich ELISA. Cell migration was assessed using wound healing assay. IL-1β significantly stimulated proliferation and migration of Hepa1-6 cells. However, expression of IL-1β significantly down-regulated growth inhibition inducecd by IFNα. The recombinant vector could stably express IL-1β and promote in vitro proliferation, migration, and impair IFNα-induced cell growth inhibition. Source


Tian J.,Key laboratory of Immunology in Universities of Shandong | Di D.,Key laboratory of Immunology in Universities of Shandong | Wang H.,Key laboratory of Immunology in Universities of Shandong | Gong F.,Key laboratory of Immunology in Universities of Shandong | And 2 more authors.
Chinese Journal of Cancer Biotherapy | Year: 2013

Objective: To study the effects of overexpression of BCSC-1 gene on the proliferation, invasion, adherence and migration abilities of hepatoma carcinoma Bel-7402 cells, and to investigate the possible mechanism. Methods: Bel-7402 cells were transfected by pcDNA3. l/v5-HisB-BCSC-l and pcDNA3. l/v5-HisB respectively as BCSC-1 group and empty vector group, and the wild-type Bel-7402 cells as a wild group. Proliferation, invasion, adherence and migration abilities of the Bel-7402 cells were detected by MTT assay, Transwell assay, adhesion experiment in vitro and scratch test, respectively. Real-time PCR was performed to detect the expression of BCSC-1 and osteopontin (OPN), ICAM-1, PTTG mRNA, which were correlated with the cell growth and adhesion in Bel-7402 cells. Results: After transfected by pcDNA3. l/v5-HisB-BCSC-l, the expression of BCSC-1 mRNA in Bel-7402 cells was significantly raised compared with that of empty vector group and the wild-type group ([10. 58 ± 0. 56] vs [1. 10 ± 0. 22], [1. 00 ± 0. 01]; all P < 0. 01). The Bel-7402 cell line stably overexpressing BCSC-1 was successfully established. Compared with the empty vector group and wild group, the growth rate of el-7402 cells in the S-1 group was obviously slower (72 h: [0.29 ± 0.003] vs [0.34 ±0.014], [0. 35 ±0.013]; all P < 0. 05), the invasion rate ([76.20 ± 1. 85]% vs [93.42 ±3.24]%, [100. 00 ± 1. 05]%; all P < 0. 01) and the adhesion rate ([58. 57 ± 0. 84]% vs [97. 14 ± 0. 84]%, [100. 00 ± 1. 30]%; all P <0. 01) were obviously decreased, the migratory distance was significantly reduced ([116. 60 ± 10. 58 J vs [231. 33 ± 10. 26], [237. 96 ± 11. 58] μm; all P < 0. 01]. The expression of OPN mRNA in the Bel-7402 cells which the BCSC-1 was up-regulated was significantly decreased ([0. 12 ± 0. 06] vs [0. 95 ± 0. 14], [1. 00 ± 0. 08]; all P <0. 01). No significant changes were observed in the expression of ICAM-1 and PTTG mRNA. Conclusion: Overex-pression of BCSC-1 can inhibit the proliferation, invasion, adherence and migration abilities of Bel-7402 cells, which may be related to the decreased expression of OPN. Source

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