Key Laboratory of Horticulture Science for Southern Mountainous Regions

Chongqing, China

Key Laboratory of Horticulture Science for Southern Mountainous Regions

Chongqing, China
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Jing L.,Southwest University | Jing L.,Key Laboratory of Horticulture Science for Southern Mountainous Regions | Jing L.,Samuel Roberts Noble Foundation | Lei Z.,Samuel Roberts Noble Foundation | And 9 more authors.
Journal of Agricultural and Food Chemistry | Year: 2014

Citrus essential oils (CEOs) are a mixture of volatile compounds consisting mainly of monoterpene hydrocarbons and are widely used in the food and pharmaceutical industries because of their antifungal activities. To face the challenge of growing public awareness and concern about food and health safety, studies concerning natural biopreservatives have become the focus of multidisciplinary research efforts. In the past decades, a large amount of literature has been published on the antifungal activity of CEOs. This paper reviews the advances of research on CEOs and focuses on their in vitro and food antifungal activities, chemical compositions of CEOs, and the methods used in antifungal assessment. Furthermore, the antifungal bioactive components in CEOs and their potential mechanism of action are summarized. Finally, the applications of CEOs in the food industry are discussed in an attempt to provide new information for future utilization of CEOs in modern industries. © 2014 American Chemical Society.


Fan S.,Shanghai University of Traditional Chinese Medicine | Zhang Y.,Shanghai University of Traditional Chinese Medicine | Hu N.,Shanghai University of Traditional Chinese Medicine | Sun Q.,Shanghai University of Traditional Chinese Medicine | And 13 more authors.
PLoS ONE | Year: 2012

Objective: To investigate the effects of ilex kudingcha C. J. Tseng (kuding tea), a traditional beverage in China, on the metabolic disorders in C57BL/6 mice induced by high-fat diets. Design: For the preventive experiment, the female C57BL/6 mice were fed with a standard diet (Chow), high-fat diet (HF), and high-fat diet mixed with 0.05% ethanol extract of kuding tea (EK) for 5 weeks. For the therapeutic experiment, the C57BL/6 mice were fed high-fat diet for 3 months, and then mice were split and EK was given with oral gavages for 2 weeks at 50 mg/day/kg. Body weight and daily food intake amounts were measured. At the end of treatment, the adipocyte images were assayed with a scanning electron microscope, and the fasting blood glucose, glucose tolerance test, serum lipid profile and lipids in the livers were analyzed. A reporter gene assay system was used to test the whether EK could act on nuclear receptor transcription factors, and the gene expression analysis was performed with a quantitative PCR assay. Results: In the preventive treatment, EK blocked the body weight gain, reduced the size of the adipocytes, lowered serum triglyceride, cholesterol, LDL-cholesterol, fasting blood glucose levels and glucose tolerance in high-fat diet-fed C57BL/6 mice. In the therapeutic treatment, EK reduced the size of the white adipocytes, serum TG and fasting blood glucose levels in obese mice. With the reporter assay, EK inhibited LXRβ transactivity and mRNA expression of LXRβ target genes. Conclusion: We observed that EK has both preventive and therapeutic roles in metabolic disorders in mice induced with high-fat diets. The effects appear to be mediated through the antagonism of LXRβ transactivity. Our data indicate that kuding tea is a useful dietary therapy and a potential source for the development of novel anti-obesity and lipid lowering drugs. © 2012 Fan et al.


Zhang Q.,Southwest University | Chen W.,Southwest University | Zhao J.,Southwest University | Zhao J.,National Goji Engineering and Technology Research Center | And 2 more authors.
Food Chemistry | Year: 2016

We quantified the levels of polyphenols, carotenoids and polysaccharides in fruits of the eight Chinese native goji genotypes, antioxidant activities of these fruit extracts were also evaluated by DPPH, ABTS and FRAP methods. Quercetin-rhamno-di-hexoside (435-1065 μg/g) and quercetin-3-O-rutinoside (159-629 μg/g) were found to be the predominant flavonoids. Chlorogenic acid was the most abundant phenolic acid (113-526 μg/g), while zeaxanthin (17-9306 μg/g) was the major carotenoid. The total antioxidant activities (TAA) of the berry extracts were significantly correlated with the total polysaccharide and phenolic contents, but not with total carotenoid (TC) levels. Overall, fruits of the Ningxia goji (Lycium barbarum L.) genotypes, DM (Damaye), NJ1 (Ningji No.1), BH (Baihua) and NH (Ningxiahuangguo) were not only rich in polyphenols, carotenoids and polysaccharides, but had significantly higher TAA than those of the other genotypes, suggesting that they represent an excellent source of antioxidants for human nutrition. © 2016 Elsevier Ltd. All rights reserved.


Jing L.,Southwest University | Jing L.,Key Laboratory of Horticulture Science for Southern Mountainous Regions | Zhang Y.,Shanghai University of Traditional Chinese Medicine | Fan S.,Shanghai University of Traditional Chinese Medicine | And 6 more authors.
European Journal of Pharmacology | Year: 2013

D-limonene is a major constituent in citrus essential oil, which is used in various foods as a flavoring agent. Recently, D-limonene has been reported to alleviate fatty liver induced by a high-fat diet. Here we determined the preventive and therapeutic effects of D-limonene on metabolic disorders in mice with high-fat diet-induced obesity. In the preventive treatment, D-limonene decreased the size of white and brown adipocytes, lowered serum triglyceride (TG) and fasting blood glucose levels, and prevented liver lipid accumulations in high-fat diet-fed C57BL/6 mice. In the therapeutic treatment, D-limonene reduced serum TG, low-density lipoprotein cholesterol (LDL-c) and fasting blood glucose levels and glucose tolerance, and increased serum high-density lipoprotein cholesterol (HDL-c) in obese mice. Using a reporter assay and gene expression analysis, we found that D-limonene activated peroxisome proliferator-activated receptor (PPAR)-α signaling, and inhibited liver X receptor (LXR)-β signaling. Our data suggest that the intake of D-limonene may benefit patients with dyslipidemia and hyperglycemia and is a potential dietary supplement for preventing and ameliorating metabolic disorders. © 2013 Elsevier B.V. All rights reserved.


Xi W.,Southwest University | Xi W.,Key Laboratory of Horticulture Science for Southern Mountainous Regions | Fang B.,Southwest University | Zhao Q.,Chinese Academy of Agricultural Sciences | And 4 more authors.
Food Chemistry | Year: 2014

China is one of the most important diversity centres of the genus Citrus L. and is particularly rich in pummelo germplasm. In this study, the flavonoids in the peels and pulps of 28 Chinese local pummelos and four grapefruits were determined by optimised Ultra Performance Liquid Chromatography (UPLC), and their antioxidant capacities were evaluated using 2,2-diphenyl-1-picrylhydrazyl radicals (DPPH), ferric reducing/antioxidant power (FRAP) and superoxide anion methods. We found that naringin was the predominant flavonoid in pummelo, while naringin and neohesperidin were the predominant flavonoids in grapefruit. The fruit peels of Citrus paradisi cvs. Rio Red and Cocktail had the highest contents of naringin (9871.69 mg/kg FW) and neohesperidin (7011.15 mg/kg FW), respectively. Overall, C. paradisi cvs. Cocktail, Rio Red and Changshanhuyou, Citrus grandis cvs. 28-19, Chandler, and Hongxinyou contained more flavonoids and exhibited higher antioxidant capacities and are potentially good sources of phytochemicals and natural antioxidants. © 2014 Elsevier Ltd. All rights reserved.


Zhao X.J.,Southwest University | Zhao X.J.,Key Laboratory of Horticulture Science for Southern Mountainous Regions | Zhang W.L.,Southwest University | Zhou Z.Q.,Southwest University | Zhou Z.Q.,Key Laboratory of Horticulture Science for Southern Mountainous Regions
Colloids and Surfaces B: Biointerfaces | Year: 2014

The citrus process industry produces annually a huge amount of pomace, which is a rich source of citrus pectin. Here, we report the hydrogel of citrus pectin mediated by sodium hydroxide can be used to prepare fluorescent carbon dots (CDs). The introduction of hydrogel can not only make the temperature of the hydrothermal reaction down to 100. °C, but also avoid visually carbonized precipitates in the synthesis process even up to 180. °C. The as-synthesized CDs are well dispersed in water with an average size of 2.7. nm and show cyan fluorescence with high photostability, good biocompatibility. Furthermore, the CDs can act as a potential fluorescent probe for cell imaging. Citrus pectin as a non-toxic carbonaceous precursor for preparation of fluorescent CDs provides a new approach for the efficient utilization of citrus germplasm in future. © 2014 Elsevier B.V.


Zhang Y.,Southwest University | Zhang Y.,Zhejiang University | Sun Y.,Zhejiang University | Xi W.,Southwest University | And 7 more authors.
Food Chemistry | Year: 2014

As one of the most important centres of origin for the genus Citrus L., China is rich in wild mandarin germplasm. In this study, phenolic compounds in the peels of 14 wild mandarin genotypes native to China were determined and their antioxidant capacities were evaluated using DPPH, FRAP, ABTS and ORAC methods. We found that Nieduyeju had the highest total phenol content (51.14 mg/g DW), and Wulongsuanju had the highest total flavonoid content (20.66 mg/g DW). Hesperidin, the dominant flavonoid, was observed to be highest in Guangxihongpisuanju (55.98 mg/g DW). Ferulic acid was the most abundant phenolic acid analyzed, and Nieduyeju (7780.17 μg/g DW) and Guangxihongpisuanju (13,607.19 μg/g DW) had the highest contents of extractable and bound phenolic acid, respectively. Antioxidant potency composite (APC) index showed obvious variations ranging from 58.84 to 98.89 in the studied wild mandarins, among them, Nieduyeju had the highest APC index. Overall, Guangxihongpisuanju, Nieduyeju, Cupigoushigan and Daoxianyeju contained more phenolics and exhibited higher antioxidant capacities than the mandarin cultivars Satsuma and Ponkan. © 2013 Published by Elsevier Ltd.


Zheng H.,Southwest University | Zhang Q.,Southwest University | Quan J.,Chongqing Nanshan Botanical Garden | Zheng Q.,Southwest University | And 2 more authors.
Food Chemistry | Year: 2016

The composition and content of sugars, organic acids, volatiles and carotenoids, in the pulps of six grapefruit cultivars, were examined by HPLC and GC-MS. The results showed that sucrose was the dominant sugar in grapefruit, making up 40.08-59.68% of the total sugars, and the ratio of fructose to glucose was almost 1:1. Citric acid was the major organic acid and represented 39.10-63.55% of the total organic acids, followed by quininic acid. The ratios of individual sugars and organic acids play an important role in grapefruit taste determination. Monoterpenes and sesquiterpenes were the predominant volatiles in grapefruit, in particular d-limonene and caryophyllene. Caryophyllene, α-humulene, humulen-(v1), β-linalool and tert-butyl 2-methylpropanoate are the characteristic aroma compounds of grapefruit. Although β-carotene is the primary carotenoid in grapefruit, the pulp color is mainly determined by the ratios of zeaxanthin, β-cryptoxanthin and lycopene. Our results provide the first complete chemical characterization of the taste, aroma and color of grapefruit. © 2016 Elsevier Ltd. All rights reserved.


Zhao X.J.,Southwest University | Zhao X.J.,Key Laboratory of Horticulture Science for Southern Mountainous Regions | Zhou Z.Q.,Southwest University | Zhou Z.Q.,Key Laboratory of Horticulture Science for Southern Mountainous Regions
Current Nanoscience | Year: 2016

Background: Pectin is a natural hetero-polysaccharide found in some fruits and vegetables with good biocompatibility and biodegradability. Besides the inherent functions of pectin in plants and its normal applications as gelling and thickening agents in food industry, pectin-based nanomaterials have attracted great attention owing to their unique properties such as the high specific surface area compared to the bulk pectin. Pectin-based nanomaterials combine the advantages of both pectin and the nanoscaled particles so that they show great potential in some other fields. Methods: Recent advance of pectin-based nanomaterials is reviewed including their synthesis strategies and applications. Results: At present, common methods based on nanoemulsion technique, ionotropic gelation, redox reactions and so on have been reported for the synthesis of pectin nanomaterials and pectin stabilized/capped nanomaterials. And these pectinbased nanomaterials can be used as gene/drug delivery system, antimicrobial agents and adsorbents for metal ions and dyes in wastewater treatment. Conclusion: The variety of nanoparticles is increasing and their applications are wider, so there are still more possibilities for us to investigate the pectin-based nanomaterials including the synthesis methods and applications in other fields. For example, the synthesis of fluorescent pectin-based nanoparticles and their applications in sensing and bioimaging. Using the advantages of both pectin and nanomaterials, we hope more pectin-based nanomaterials can be designed and synthesized and further applied in desired areas. © 2016 Bentham Science Publishers.


Gu M.,Shanghai University of Traditional Chinese Medicine | Zhang Y.,Shanghai University of Traditional Chinese Medicine | Fan S.,Shanghai University of Traditional Chinese Medicine | Ding X.,Shanghai University of Traditional Chinese Medicine | And 4 more authors.
PLoS ONE | Year: 2013

Polygonatum odoratum (Mill.) Druce belongs to the genus Polygonatum family of plants. In traditional Chinese medicine, the root of Polygonatum odoratum, Rhizoma Polygonati Odorati, is used both for food and medicine to prevent and treat metabolic disorders such as hyperlipidemia, hyperglycemia, obesity and cardiovascular disease. However, there is no solid experimental evidence to support these applications, and the underlying mechanism is also needed to be elucidated. Here, we examined the effect of the extract of Rhizoma Polygonati Odorati (ER) on metabolic disorders in diet-induced C57BL/6 obese mice. In the preventive experiment, the ER blocked body weight gain, and lowered serum total cholesterol (TC), triglyceride (TG) and fasting blood glucose, improved glucose tolerance test (GTT) and insulin tolerance test (ITT), reduced the levels of serum insulin and leptin, and increased serum adiponectin levels in mice fed with a high-fat diet significantly. In the therapeutic study, we induced obesity in the mice and treated the obese mice with ER for two weeks. We found that ER treatments reduced serum TG and fasting blood glucose, and improved glucose tolerance in the mice. Gene expression analysis showed that ER increased the mRNA levels of peroxisome proliferator-activated receptors (PPAR) γ and α and their downstream target genes in mice livers, adipose tissues and HepG2 cells. Our data suggest that ER ameliorates metabolic disorders and enhances the mRNA expression of PPARs in obese C57BL/6 mice induced by high-fat diet. © 2013 Gu et al.

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