Entity

Time filter

Source Type


Xu L.,Northwest University, China | Xu L.,Key Laboratory of Horticultural Plant Germplasm Resource Utilization in Northwest China | Du Y.,Northwest University, China | Du Y.,Key Laboratory of Horticultural Plant Germplasm Resource Utilization in Northwest China
BioControl | Year: 2012

The yeast antagonist Candida guilliermondii and ultraviolet-C (UV-C) treatment were investigated for controlling infection following artificial inoculation with Penicillium expansum or Botrytis cinerea, or natural infection in pear fruit stored at 20°C. Applied separately, both C. guilliermondii and UV-C (5 kJ m -2) effectively inhibited decay caused by P. expansum or B. cinerea, and natural infection. The combination of C. guilliermondii and UV-C showed better control efficacy. Application of UV-C did not affect the growth of C. guilliermondii in pear fruit wounds, while UV-C induced a significant increase in the activities of chitinase, β-1,3-glucanase, catalase and peroxidase in pear fruit. The mechanism by which UV-C enhanced the biocontrol efficacy of C. guilliermondii may be related to the elicitation of defense responses in pear fruit. The combination of C. guilliermondii and UV-C radiation could be a promising method for the control of P. expansum and B. cinerea in pear fruit. © 2011 International Organization for Biological Control (IOBC). Source


Li C.,Northwest University, China | Li C.,Key Laboratory of Horticultural Plant Germplasm Resource Utilization in Northwest China | Bai T.,Northwest University, China | Bai T.,Key Laboratory of Horticultural Plant Germplasm Resource Utilization in Northwest China | And 3 more authors.
Scientia Horticulturae | Year: 2010

Seedlings of two Malus species (M. hupehensis and M. toringoides) were hydroponically grown in normoxic and hypoxic nutrient solutions to determine their hypoxia tolerance and adaptation of root anaerobic respiration to hypoxia stress. The growth of both species was inhibited under hypoxia stress. During hypoxia stress, the generation of superoxide anion radical (O2{radical dot}-) and content of hydrogen peroxide (H2O2) in roots were significantly increased in both species with similar trends, which led to an increase of malondialdehyde (MDA) content and relative membrane permeability (RMP). The degree of growth inhibition and the levels of O2{radical dot}-, H2O2, MDA and RMP were greater in M. toringoides than in M. hupehensis. Under hypoxia stress, pyruvate decarboxylase (PDC), alcohol dehydrogenase (ADH) and lactate dehydrogenase (LDH) activities in roots were increased during the first 12 days of hypoxia stress and then gradually decreased in both species. The contents of acetaldehyde, alcohol and lactate in roots were also increased and showed similar trends as the activities of anaerobic respiration enzymes. The increases in PDC and ADH activities and lactate content in M. hupehensis under hypoxia conditions were greater than those of M. toringoides, but alcohol and acetaldehyde contents showed opposite trends. These data suggest that M. hupehensis is more tolerant of hypoxia and had less damage from oxidative stress than M. toringoides under hypoxia stress. The capability for anaerobic respiration is up-regulated in roots of Malus in response to hypoxia stress to minimize damage, and the higher hypoxia tolerance of M. hupehensis may be partly due to the higher enzyme activity of PDC, ADH and LDH and lower accumulation of acetaldehyde and alcohol. © 2010 Elsevier B.V. All rights reserved. Source


Bai T.,Northwest University, China | Bai T.,Key Laboratory of Horticultural Plant Germplasm Resource Utilization in Northwest China | Li C.,Northwest University, China | Li C.,Key Laboratory of Horticultural Plant Germplasm Resource Utilization in Northwest China | And 6 more authors.
Plant and Soil | Year: 2010

Responses of growth and antioxidant system to root-zone hypoxia stress were comparatively studied in two Malus species (M. hupenensis and M. toringoides) differing in hypoxia tolerance. 50-day-old seedlings were hydroponically grown for 20 days in normoxic and hypoxic nutrient solutions. Hypoxia stress inhibited the growth of both species. Compared with M. hupenensis, M. toringoides was more responsive to hypoxia stress, resulting in larger decreases in leaf number, root length, plant height, and biomass production. The contents of superoxide radicals (O2-) and hydrogen peroxide (H2O2) significantly increased in roots of both species exposed to hypoxia stress, and resulted in lipid peroxidation, which was indicated by accumulated concentration of malonaldehyde (MDA). In addition, a significant increase in O2-, H2O2 and MDA contents was found in M. toringoides under hypoxia stress. In responses to hypoxia stress, peroxidse (POD), superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) activities increased during the early part of the hypoxia stress, but decreased in the late period; the activities of SOD, POD and APX were more increased in M. hupenensis than in M. toringoides. Ascorbic acid (AsA) and glutathione (GSH) accumulation was also higher in M. hupenensis than in M. toringoides in the early period under hypoxia stress. These results suggest that the hypoxia-tolerant M. hupenensis has a larger protective capacity against oxidative damage by maintaining higher induced activities of antioxidant system than the hypoxia-sensitive M. toringoides. © Springer Science + Business Media B.V. 2009. Source


Wang Y.,Northwest University, China | Wang Y.,Key Laboratory of Horticultural Plant Germplasm Resource Utilization in Northwest China | Ma F.,Northwest University, China | Ma F.,Key Laboratory of Horticultural Plant Germplasm Resource Utilization in Northwest China | And 6 more authors.
Plant Growth Regulation | Year: 2011

We studied the physiological responses to abscisic acid (ABA) when 2-year-old potted plants of kiwifruit (Actinidia deliciosa) were grown under moisture stress. Leaves treated with 60 μM exogenous ABA through various means had less severe damage when water was limiting, and sprayed plants showed relatively greater drought resistance. This indicates that ABA improves tolerance in kiwifruit, reducing membrane permeability and enhancing the activities of antioxidant enzymes, e. g., peroxidase (POD), catalase (CAT), superoxide dismutase (SOD), ascorbate peroxidase (APX), and glutathione reductase (GR). Exposure to ABA led to higher levels of antioxidants, such as ABA and glutathione (GSH), while altering the amounts of endogenous hormones-ABA, indole-3-acetic acid (IAA), and Gibberellin (GA)-and organic oxalate, malate, and citrate in the leaves. Although daily applications of ABA were more effective than a single spray event, the effect of treatment, i. e., avoiding tissue damage and increasing plant resistance, was more apparent on Day 4 than on Day 6. No difference in response was apparent between control plants (regular irrigation) and those sprayed with ABA on Day 4 of the drought period. © 2010 Springer Science+Business Media B.V. Source


Xu Y.,Northwest University, China | Xu Y.,Key Laboratory of Horticultural Plant Germplasm Resource Utilization in Northwest China | Yu H.,Northwest University, China | Yu H.,Key Laboratory of Horticultural Plant Germplasm Resource Utilization in Northwest China | And 6 more authors.
Biologia | Year: 2010

A novel PR10 gene (designated as VpPR10) was isolated from Chinese wild Vitis pseudoreticulata W. T. Wang Baihe-35-1. VpPR10 encoded a 159-amino-acid polypeptide with a predicted molecular mass of 17.46 kDa. Sequence alignment showed that VpPR10 had high similarity with the PR10 proteins of other plant species. Genomic DNA gel blot analysis indicated that VpPR10 belonged to a multi-gene family in Chinese wild Vitis. Quantitative real-time PCR analysis exhibited that the transcript level of VpPR10 altered at different times after inoculation with Uncinula necator under natural field conditions. VpPR10 expression began to decrease after 24 h and reached the lowest level after 72 h. Then it began to increase and obtained the normal level after 96 h. After expression in Escherichia coli and purification, recombinant VpPR10 protein was used to immune rabbit to obtain polyclonal antibodies. Immunoblot analysis showed that the expression pattern of VpPR10 protein was similar with that of VpPR10 mRNA. © 2010 Versita Warsaw and Springer-Verlag Wien. Source

Discover hidden collaborations