Key Laboratory of Hormone and Development

Tianjin, China

Key Laboratory of Hormone and Development

Tianjin, China

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Peng X.-X.,Weifang Medical University | Zhao R.-L.,Weifang Medical University | Song W.,Weifang Medical University | Chu H.-R.,Weifang Medical University | And 5 more authors.
International Journal of Molecular Sciences | Year: 2012

When studying the altered expression of genes associated with cartilage regeneration by quantitative real-time RT-PCR (RT-qPCR), reference genes with highly stable expression during different stages of chondrocyte developmental are necessary to normalize gene expression accurately. Until now, no reports evaluating expression changes of commonly used reference genes in rabbit articular cartilage have been published. In this study, defects were made in rabbit articular cartilage, with or without insulin-like growth factor 1 (IGF-1) treatment, to create different chondrocyte living environments. The stability and intensity of the expressions of the candidate reference genes glyceraldehyde-3-phosphate dehydrogenase (GAPDH), 18S Ribosomal RNA (18S rRNA), cyclophilin (CYP), hypoxanthine phosphoribosyl transferase (HPRT1), and β-2-microglobulin (B2M) were evaluated. The data were analyzed by geNorm and NormFinder. B2M and 18S rRNA were identified to be suitable reference genes for rabbit cartilage tissues. © 2012 by the authors; licensee MDPI, Basel, Switzerland.

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