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Zhang X.-X.,National Engineering Laboratory for Trees Breeding | Zhang X.-X.,Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education | Zhang X.-X.,Tree and Ornamental Plant Breeding and Biotechnology Laboratory of State Forestry Administration | Zhang X.-X.,Beijing Forestry University | And 14 more authors.
Beijing Linye Daxue Xuebao/Journal of Beijing Forestry University | Year: 2010

The optimal tissue culture conditions for induction, differentiation, proliferation and rooting of adventitious buds of Pueraria lobata Ohwi were studied through in vitro culture and rapid propagation technologies. The results showed that using stem segments with axillary buds from elite parent trees of P. lobata as explants, the survival rate could reach 50% after 25-30 min of sterilization with 2.2% NaClO. The most optimal medium for induction of adventitious buds was MS + NAA 0.1 mg/L + 6-BA 0. 5 mg/L, and the induction rate reached 88.89%. The most optimal medium for differentiation and subculture of the adventitious buds was MS + NAA 0.15 mg/L + 6-BA 0.3 mg/L + 2, 4-D 0.2 mg/L; the differentiation rate reached 83.33%, and the multiples of proliferation rate ranged from 6 to 7. The most optimal rooting medium was 1/2 MS supplemented with NAA 0.5 mg/L, and the rooting rate could reach 100%. Overall, a tissue culture system and a relatively good technological system for rapid propagation of P. lobata were established.


Huang J.-C.,Forestry Technique Promotion Station of Baoshan City | He J.,CAS Kunming Institute of Botany | Yin R.-P.,Forestry Technique Promotion Station of Baoshan City | Wan X.-J.,Forestry Technique Promotion Station of Baoshan City | And 6 more authors.
Beijing Linye Daxue Xuebao/Journal of Beijing Forestry University | Year: 2010

For the purpose of improving the fruit and seed traits by selection, 12 fruit and seed traits of sample trees from 3 natural stands and 6 artificial plantations with large size in Camellia reticulata L. were analyzed. The results showed that there were significant variations among genesis, seed sources and individual trees for all traits, except for diameters of fruit. The general rankings in percentage of variance component for different resources were individual, seed resource and genesis. The correlative coefficients between length against width of fruit and other seed traits, as well as oil content indices were negative significant. The correlative coefficients between seed traits and oil content indices were positive significant. The traits as length against width of fruit, seed weight per fruit, kernel ratio per seed, oil content per seed and oil content per fruit were with much higher variations for each resource and higher values for improvement by selection. There were significant negative correlations between length against width of fruit, elevation and oil content of fruit. The traits and strategy for selection in C. reticulata were suggested. Selection of populations and elite trees with higher value in tea-oil production potential should be much benefit for further development of tea-oil industry of C. reticulata in China.


Ding Y.-F.,National Engineering Laboratory for Tree Breeding | Ding Y.-F.,Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education | Ding Y.-F.,Tree and Ornamental Plant Breeding and Biotechnology Laboratory of State Forestry Administration | Ding Y.-F.,Beijing Forestry University | And 15 more authors.
Beijing Linye Daxue Xuebao/Journal of Beijing Forestry University | Year: 2010

Diurnal changes of leaf net photosynthetic rates of two cultivars of Pueraria lobata were measured using the CIRAS-I portable photosynthesis system (PP-Systems Co., British), and the relationship among diurnal changes of net photosynthetic rates and those of environmental factors were analyzed. The results showed that diurnal changes in the net photosynthetic rates of both two cultivars could be interpreted as double-peak curves, i. e. they possessed obvious so-called "midday depression" phenomenon. Further analyses showed that the correlation between net photosynthetic rate and stomatal conductance was positive and extremely significant (P < 0.01), the correlations between net photosynthetic rate and intercellular CO 2 concentration as well as the transpiration rate were positive and significant (P < 0.05), and the correlations between net photosynthetic rate and air temperature as well as leaf temperature were negative and significant (P < 0.05). The results indicate that among the factors affecting photosynthetic characteristics, some of them could be grouped as stomatal restrictions, while the others could be grouped as non-stomatal restrictions.


Li Y.,National Engineering Laboratory for Tree Breeding | Li Y.,Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education | Li Y.,Tree and Ornamental PlantBreeding and Biotechnology Laboratory of State Forestry Administration | Li Y.,Beijing Forestry University | And 13 more authors.
Beijing Linye Daxue Xuebao/Journal of Beijing Forestry University | Year: 2010

Flowering synchronization is essential for genetic management and deployment of clones in seed orchard. Studying variation and stability of flowering synchronization of clones among plant ages are helpful for further management of clones in present seed orchard and backward selection. Based on collected data of florescence period and flower number of 49 clones within a 13-year span in a seed orchard of Pinus tabulaeformis, the flowering synchronization of each clone in different investigated ages was estimated. Results showed that there were obvious differences of flowering synchronization among parent clone combinations. In general, flowering synchronization of clone as paternal was higher than it as maternal. Probability of selfing within clones were higher than out-crossing. There were moderate stability of flowering synchronization of clone as material among plant ages. So, clone selection by flowering synchronization of clone as material would be valuable in early flowering ages in seed orchard, but flowering synchronization of clone as paternal might be lower. Analysis of flowering synchronization of clones would be benefit for clone management in present seed orchard and refer to both genetic design and collocating design of parent for advanced seed orchard based on backward selection of clones.


Xu X.,National Engineering Laboratory for Tree Breeding | Xu X.,Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education | Xu X.,Tree and Ornamental Plant Breeding and Biotechnology Laboratory of State Forestry Administration | Xu X.,Beijing Forestry University | And 5 more authors.
Beijing Linye Daxue Xuebao/Journal of Beijing Forestry University | Year: 2010

In this report, the expression of AsEXP1 gene was tested under drought stress among turf-grass species. The result showed significant correlation between AsEXP1 gene expression and drought tolerance. It is expressed in drought tolerant plants but silent in drought sensitive plants. Further analysis with turf-grass 'PennA-4', a heat tolerant but drought sensitive grass species, revealed that the induced AsEXP1 by heat was beneficial for plants to tolerate drought stress.


Xu L.-L.,National Engineering Laboratory for Tree Breeding | Xu L.-L.,Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education | Xu L.-L.,Tree and Ornamental Plant Breeding and Biotechnology Laboratory of State Forestry Administration | Xu L.-L.,Beijing Forestry University | And 12 more authors.
Beijing Linye Daxue Xuebao/Journal of Beijing Forestry University | Year: 2010

Using ovules of the female-sterile Pinus tabulaeformis Carr. mutant and the fertile, which has the similar genetic background, as materials, the total RNA was extracted with Plant RNA assistant kit. The first-strand of cDNA synthesis and double-strands cDNA amplification were performed by Super SMART cDNA synthesis technology. The quality of cDNA was identified by gradient PCR amplification cycles and electrophoresis. Their amplification efficiency was evaluated by the quantitative method. A total of 46.06 and 43.59μg purified double-strands cDNA were amplified successfully from 1μg total RNA, respectively, which had high quality and purity. The results indicate that Super SMART cDNA synthesis technology could amplify enough high quality cDNA from trace of total RNA. This method solves the problem of limited research materials and builds up the foundation of suppressed subtractive hybridization in future.


Song Y.-P.,National Engineering Laboratory for Tree Breeding | Song Y.-P.,Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education | Song Y.-P.,Beijing Forestry University | Jiang X.-B.,National Engineering Laboratory for Tree Breeding | And 15 more authors.
Beijing Linye Daxue Xuebao/Journal of Beijing Forestry University | Year: 2010

Sixteen poplar clones were used to analyse the genetic differences between Genomic-SSR and EST-SSR. The statistical results showed that the average number of alleles detected by Genomic-SSR was 4.1, Shannon was 1.064 6, heterozygosity was 0.4427, expected heterozygosity was 0.5523; while for the EST-SSR, the average number of alleles was 2.8, Shannon was 0.698 5, heterozygosity was 0.233 0, expected heterozygosity was 0.468 4. Cluster analysis indicated that the EST-SSR capacity of genotypes identification was more precise than Genomic-SSR. In a word, all these results reveal that the EST-SSR and Genomic-SSR have significant genetic differences in markers polymorphism and genotype identification, and that the differences would provide theoretical basis for the rational use of SSR marker in species diversity and other related research.


Hao J.-Q.,National Engineering Laboratory for Tree Breeding | Hao J.-Q.,Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education | Hao J.-Q.,Tree and Ornamental Plant Breeding and Biotechnology Laboratory of State Forestry Administration | Hao J.-Q.,Beijing Forestry University | And 20 more authors.
Beijing Linye Daxue Xuebao/Journal of Beijing Forestry University | Year: 2010

The changes of chlorophyll fluorescence parameters and free water and bound water of three typical heteromorphic leaves of Populus euphratica, both in young trees and old trees, were investigated. The results showed that the values of F0, Fm, Fv/Fm and Fv/F0 of lanceolate leaves were the least in young trees, while those of Fm, Fv/F0 and Fv/Fm of ovate leaves were the least in old trees. Compared with the young trees, the free water content of heteromorphic leaves of old trees increased significantly by 78.94% in lanceolate leaves, and in the leaves of ovate and serrated broad-oval, it increased by 10.60% and 10.99% respectively. The analysis of correlations showed that the free water content was significantly positive related with Fv/Fm and Fv/F0 in young trees, while the relationship of total water content with Fv/Fm and Fv/F0 was positive in old trees.


Ouyang F.-Q.,National Engineering Laboratory for Tree Breeding | Ouyang F.-Q.,Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education | Ouyang F.-Q.,Tree and Ornamental Plant Breeding and Biotechnology Laboratory of State Forestry Administration | Ouyang F.-Q.,Beijing Forestry University | And 9 more authors.
Beijing Linye Daxue Xuebao/Journal of Beijing Forestry University | Year: 2010

To understand the seedling trait performance from different black spruce (Picea mariana) provenances with varied light treatments and to establish efficient propagation technique system with supplemental light, container seedlings of 10 black spruce provenances from Canada were treated by 3 light sources of fluorescent lamp, sunlight dysprosium lamp and tungsten lamp. The results showed that light treatment could inhibit seedling dormancy and promote sustaining growth. The seedling height by using fluorescent light for 8 hrs at midnight for 135 days could be up to 5.47 times compared with control. The effect of light sources was significant on seedling growth, shoot and root formation. The treatment for sunlight dysprosium lamps was best, and fluorescent lamp was more economical and convenient. Besides, the seedling grew well when fluorescent lamp was supplemented for 8 hrs at midnight. There were significant interaction effects on seedling traits between light sources and provenances. It was indicated that there were obvious differences for different black spruce provenances in adapting to light environment. Therefore, it should be paid more attention to select appropriate provenances for introduction experiment. The light source should be selected according to the seedling provenances to achieve the optimum breeding results.


Gao H.,Beijing Forestry University | Gao H.,National Engineering Laboratory for Tree Breeding | Gao H.,Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education | Gao F.,Beijing Forestry University
Frontiers in Biology | Year: 2011

Chloroplasts are photosynthetic organelles derived from endosymbiotic cyanobacteria during evolution. Dramatic changes occurred during the process of the formation and evolution of chloroplasts, including the large-scale gene transfer from chloroplast to nucleus. However, there are still many essential characters remaining. For the chloroplast division machinery, FtsZ proteins, Ftn2, SulA and part of the division site positioning system-MinD and MinE are still conserved. New or at least partially new proteins, such as FtsZ family proteins FtsZ1 and ARC3, ARC6H, ARC5, PDV1, PDV2 and MCD1, were introduced for the division of chloroplasts during evolution. Some bacterial cell division proteins, such as FtsA, MreB, Ftn6, FtsW and FtsI, probably lost their function or were gradually lost. Thus, the chloroplast division machinery is a dynamically evolving structure with both conservation and innovation. © 2011 Higher Education Press and Springer-Verlag Berlin Heidelberg.

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