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Wei L.,Key Laboratory of Freshwater Fishery Germplasm Resources | Huang X.,Key Laboratory of Freshwater Fishery Germplasm Resources | Huang X.,Shanghai Ocean University | Huang Z.,Key Laboratory of Freshwater Fishery Germplasm Resources | Zhou Z.,Key Laboratory of Freshwater Fishery Germplasm Resources
Bioresource Technology | Year: 2013

In order to improve the property of Nannochloropsis oculata as biodiesel feedstock, a L9(34) orthogonal test on limited nitrogen supplementation (0, 0.22 and 0.44mmolNL-1), high iron concentration (1.2×10-2, 1.2×10-1 and 1.2mmolFeL-1) and culture temperature (10, 20 and 30°C) was conducted to select the most effective combinational measurement. Results showed that microalgae displayed the highest total lipid content (60.44±0.68%), the highest neutral lipid proportion (90.74±0.18%), the highest lipid yield (152.70±7.40mgL-1) and the largest cetane number (CN, 64.34±0.13) under different combined conditions. There were significant interaction among nitrogen supplementation, iron concentration and culture temperature on the lipid yield and CN of N. oculata. Nitrogen supplementation, followed by iron concentration and temperature orderly, was the most influential factor in lipid yield. It is therefore suggested that the combination of 0.44mmolNL-1, 1.2×10-1mmolFeL-1 and 20°C was the best measurement for improving the property of N. oculata as biodiesel feedstock. © 2013. Source

Wei L.,Key Laboratory of Freshwater Fishery Germplasm Resources | Huang X.,Key Laboratory of Freshwater Fishery Germplasm Resources | Huang X.,Shanghai Ocean University | Huang Z.,Key Laboratory of Freshwater Fishery Germplasm Resources
Chinese Journal of Oceanology and Limnology | Year: 2014

Microalgae Tetraselmis subcordiformis and Nannochloropsis oculata were cultured at 15, 20, 25, 30, and 35°C and their properties as potential biofuel resources were examined. The results indicate that T. subcordiformis and N. oculata grew best at 20°C and 25°C and yielded the highest total lipids at 20°C and 30°C, respectively. With increased temperature, neutral lipid and polyunsaturated fatty acids (FAs) decreased while saturated FAs increased, accompanied by increased monounsaturated FAs (MUFAs) in T. subcordiformis and decreased MUFAs in N. oculata; meanwhile, the predicted cetane number of FA methyl esters increased from 45.3 to 47.6 in T. subcordiformis and from 52.3 to 60.3 in N. oculata. Therefore, optimizing culture temperatures is important for improving microalgal biodiesel production. © 2015, Chinese Society for Oceanology and Limnology, Science Press and Springer-Verlag Berlin Heidelberg. Source

Hu J.,Shanghai Ocean University | Guo T.,Shanghai Ocean University | Pan W.-Q.,Shanghai Ocean University | Gan T.,Shanghai Ocean University | And 5 more authors.
Journal of Muscle Research and Cell Motility | Year: 2016

Unc45 myosin chaperone b(unc45b)gene is a molecular chaperone that mediates the folding, assembly and accumulation of thick-filament myosin in the formation of sarcomere, which plays an important role in the development of striated muscle and the stability of sarcomere. In this study, the complete cDNA sequence of unc45b gene of grass carp was obtained by rapid amplification of cDNA ends (RACE), and the characteristics of the unc45b protein predicted from gene sequence was analyzed by bioinformatics methods. The differential expression pattern in tissues was also detected by quantitative real-time PCR. The results showed that the full-length of unc45b gene of grass carp is 3163 bp, which contains a 60 bp 5′UTR, a 298 bp 3′UTR, and a 2865 bp open reading frame (ORF) encoding a 934 amino acid peptide. The deduced unc45b protein exhibits a homology of 92, 86, 86 % with the protein of zebrafish (Danio rerio), channel catfish (Ietalurus punctatus) and tilapia (Oreochromis niloticus) respectively, and the protein contains UCS myosin head binding domain and TPR peptide repeat domain. The protein is a hydrophilic and non-secretory protein with a molecular mass and isoeletronic point of 103,699.8 and 7.39 Da. The structural elements of the protein includes α-helixes and loops, and the unc45b gene highly expresses in skeletal muscle and heart in grass carp. This study laid a foundation for further research in explaining the myofibril accumulation in crisped grass carp. © 2016 Springer International Publishing Switzerland Source

Yang J.-L.,Shanghai Ocean University | Yang J.-L.,Key Laboratory of Freshwater Fishery Germplasm Resources | Li W.-S.,Shanghai Ocean University | Liang X.,Shanghai Ocean University | And 4 more authors.
Aquaculture | Year: 2014

The metamorphosis responses of mussel (Mytilus coruscus) larvae to adrenoceptor compounds were investigated through a series of bioassays. The adrenergic agonist, epinephrine, as a positive control, exhibited significant inducing activity. Phenylephrine and clonidine induced larval metamorphosis at 10-6 to 10-4M concentrations in both 24-h and continuous exposure assays. Dobutamine induced larval metamorphosis at 10-5M in the 24-h exposure assays, and the percentage of larval metamorphosis was very low (<5%). Methoxyphenamine exhibited inducing activity at 10-4M in the continuous exposure assays, and the percentage of larval metamorphosis was 8%. No larval mortality was observed for these five agonists at all concentrations tested. Among these antagonists, α1-adrenergic receptor antagonist chlorpromazine and amitriptyline showed by far the most promising inhibiting effects, indicating that G-protein-coupled α1-adrenoceptors may be involved in the process of larval metamorphosis in M. coruscus. The α2-adrenergic receptor antagonist idazoxan also inhibited the larval metamorphosis of M. coruscus, indicating that larval metamorphosis may also be mediated by G-protein-coupled α2-adrenoceptors. Atenolol and butoxamine also inhibited larval metamorphosis induction by epinephrine. Thus, these adrenergic agonists can be used as non-toxic and promising inducers of larval metamorphosis in this species, and to improve M. coruscus larval production for aquaculture. The present study provides a novel insight into the mechanism modulating the metamorphosis of larvae of the mussel M. coruscus. © 2014 Elsevier B.V. Source

Ma F.,Shanghai Ocean University | Li X.Q.,Shanghai Ocean University | Li B.A.,Shanghai Ocean University | Leng X.J.,Shanghai Ocean University | Leng X.J.,Key Laboratory of Freshwater Fishery Germplasm Resources
Aquaculture Nutrition | Year: 2016

This study was conducted to evaluate the effects of extruded diets and pelleted diets with varying dietary lipid levels on growth performance and nutrient utilization of tilapia. Six diets, containing three levels of lipid at 40, 60 or 80 g kg-1 (with the supplemental lipid of 0, 20 or 40 g kg-1, respectively), were prepared by extruding or pelleting and then fed to tilapia juveniles (8.0 ± 0.1 g) in cages (in indoor pools) for 8 weeks. The results indicated that the fish that were fed the diet with 60 g kg-1 of lipid had a higher weight gain (WG), specific growth rate (SGR), protein efficiency ratio (PER), lipid retention (LRE), energy retention (ERE), apparent protein digestibility, apparent dry matter digestibility and a lower feed conversion ratio (FCR) than those fed the diet with 40 g kg-1 lipid in both the extruded diet and pelleted diet (P < 0.05). As the dietary lipid level increased from 60 to 80 g kg-1, these parameters were not further improved, even digestibilities of the crude protein and dry matter decreased (P < 0.05). With the dietary lipid level increased, whole-body lipid content significantly increased (P < 0.05), serum aspartate aminotransferase, alkaline phosphatase, total cholesterol and low-density lipoprotein cholesterol (LDL-C) tended to increase (P > 0.05), whereas whole-body protein content, serum triglyceride (TG), high-density lipoprotein cholesterol (HDL-C) and HDL-C/LDL-C tended to decrease (P > 0.05). Fish fed with the extruded diets had a higher WG, SGR, hepatosomatic index (HSI), PER, protein retention (PRE), LRE, ERE, TG, apparent digestibility of protein and dry matter, as well as a lower FCR, than those fed with the pelleted diets at the same dietary lipid level (P < 0.05). These results suggested that tilapia fed with the extruded diets had a better growth and higher nutrient utilization than fish fed with the pelleted diets, when dietary lipid level ranged from 40 to 80 g kg-1 and at dietary crude protein level was 280 g kg-1. The optimum dietary lipid level was 60 g kg-1 in both the pelleted and extruded diets, and extrusion did not affect dietary lipid requirement of the tilapia. © 2016 John Wiley & Sons Ltd. Source

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