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Zhang W.-L.,Key Laboratory of Freshwater Fish Reproduction and Development Ministry of Education | Zhou L.-Y.,Japan National Institute for Basic Biology | Senthilkumaran B.,Japan National Institute for Basic Biology | Senthilkumaran B.,University of Hyderabad | And 8 more authors.
General and Comparative Endocrinology | Year: 2010

P450 11β-hydroxylase, encoded by P450(11β) gene, is a key mitochondrial enzyme to produce 11β-hydroxy testosterone, substrate for the production of 11-ketotestosterone (11-KT), which has been shown to be potent androgen in several fish species. In the present work, two alternative splicing isoforms i.e. P450(11β)-1 and P450(11β)-2 cDNAs were cloned from the Nile tilapia, Oreochromis niloticus. They were 1614 and 1227 bp in length with open reading frames encoding proteins of 537 and 408 amino acids, respectively. In contrast to P450(11β)-1, which derived from 9 exons of the P450(11β) gene, the 7th and 8th exons were absent in P450(11β)-2. Tilapia P450(11β)-1 shares the highest homology with that of medaka, Oryzias latipes. Expressions of P450(11β)-1 and -2 were detected in the kidney and head kidney of both sexes, and in the testis but not in the ovary, with P450(11β)-2 lower than P450(11β)-1. Ontogenic expressions of both isoforms were detected in testis from 50dah onwards. P450(11β)-1 and -2 were strongly expressed in sex reversed XX testis after fadrozole and tamoxifen treatment, but completely inhibited in 17β-estradiol induced XY ovary. The existence of two alternatively spliced isoforms and the sexual dimorphic expression of P450(11β)s were further confirmed by Northern blot. Strong expression signals in Leydig cells and weak signals in spermatogonia were detected by in situ hybridization and immunohistochemistry. Taken together, our data suggest a role for P450(11β) in the spermatogenesis of tilapia through the production of 11-KT in testis, in addition to cortisol production in head kidney. © 2009 Elsevier Inc. All rights reserved. Source

Ma X.,Key Laboratory of Freshwater Fish Reproduction and Development Ministry of Education | Ma X.,Southwest University | Huang F.,Key Laboratory of Freshwater Fish Reproduction and Development Ministry of Education | Huang F.,Southwest University | And 2 more authors.
Mitochondrial DNA | Year: 2014

The complete mitogenome sequence of Spinibarbus sinensis was determined using long PCR reactions. The genome is 16,591bp in length, including 13 typical vertebrate protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes and a control region. Except for eight tRNA and ND6 genes, all other mitochondrial genes are encoded on the heavy strand. The gene order and composition of S. sinensis was similar to that of most other vertebrates. The descending order of the base composition on heavy strand was 32.0% A, 25.5% T, 26.6% C, 15.9% G, with a relatively lower level of G and a slight AT bias of 57.5%. The codon usage followed the typical vertebrate mitochondrial pattern (ATG or GTG for start codon and TAA or TAG for stop codon). There are 10 regions of gene overlap totaling 30bp and 13 intergenic spacer regions totaling 69bp. © 2014 Informa UK Ltd. All rights reserved. Source

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