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Yang Y.-C.,Forestry Academy of Jilin province | Li J.,Key Laboratory of Forest Plant Ecology | Fu Y.-J.,Key Laboratory of Forest Plant Ecology | Zu Y.-G.,Key Laboratory of Forest Plant Ecology
Chemistry and Industry of Forest Products | Year: 2013

Seasonal variation of corilagin, geraniin as well as total phenolic contents during the growth period, where were obtained from the whole grass, roots, stems and leaves of Geranium sibiricum Linne, were investigated. The antioxidant capacity (FRAP, DPPH, ABTS) were evaluated to confirm the optimum picking part and stage. Results showed that corilagin (CG), geraniin (GE) and total phenolic contents (TPC) increased dramatically in the vegetative growth period (137.5673±1.8907 GAE/g extracts)and decreased gradually during the reproductive growth and withering period (74.7518±1.0101 GAE/g extracts), while the total flavonoid contents (TF) was high in summer and reached the highest during the withering period. The optimum picking part and time were the whole grass and July, respectively, and the total antioxidant capacity (FRAP) was (12.3215±0.1882) mmol/g extracts. Correlation of the data obtained from principal composition analysis (PCA) showed that TPC, CG and GE had high relationship (r>0.753) and they also had high relationship with antioxidant capacity (|r|>0.75). Source


Wei Z.-F.,Key Laboratory of Forest Plant Ecology | Wei Z.-F.,Northeast Forestry University | Jin S.,Key Laboratory of Forest Plant Ecology | Jin S.,Northeast Forestry University | And 13 more authors.
Journal of Agricultural and Food Chemistry | Year: 2013

Pigeon pea is an important and multiuse grain legume crop, and its leaves are a very valuable natural resource. To obtain a high-quality biological resource, it is necessary to choose the excellent cultivar and determine the appropriate harvest time. In this study, the variation in contents of main active components and antioxidant activity in leaves of six pigeon pea cultivars during growth were investigated. The level of each individual active component significantly varied during growth, but with a different pattern, and this variation was different among cultivars. Flavonoid glycosides orientin, vitexin, and apigenin-6,8-di-C-α-l-arabinopyranoside showed two peak values at mid-late and final stages of growth in most cases. Pinostrobin chalcone, longistyline C, and cajaninstilbene acid showed remarkablely higher values at the mid-late stage of growth than at other stages. Pinostrobin had an extremely different variation pattern compared to other active components. Its content was the highest at the earlier stage of growth. Principal component analysis (PCA) revealed that vitexin and apigenin-6,8-di-C-α-l-arabinopyranoside were mainly responsible for distinguishing cultivars analyzed. In a comprehensive consideration, the leaves should preferentially be harvested at the 135th day after sowing when the level of active components and antioxidant activity reached higher values. Cultivars ICP 13092, ICPL 87091, and ICPL 96053 were considered to be excellent cultivars with high antioxidant activity. Our findings can provide valuable information for producing a high-quality pigeon pea resource. © 2013 American Chemical Society. Source


Li Y.,Key Laboratory of Forest Plant Ecology | Wang H.,Key Laboratory of Forest Plant Ecology | Wang W.,Key Laboratory of Forest Plant Ecology | Yang L.,Key Laboratory of Forest Plant Ecology | Zu Y.,Key Laboratory of Forest Plant Ecology
The Scientific World Journal | Year: 2013

Limited data are available on the ectomycorrhizae-induced changes in surface structure and composition of soil colloids, the most active portion in soil matrix, although such data may benefit the understanding of mycorrhizal-aided soil improvements. By using ectomycorrhizae (Gomphidius viscidus) and soil colloids from dark brown forest soil (a good loam) and saline-alkali soil (heavily degraded soil), we tried to approach the changes here. For the good loam either from the surface or deep soils, the fungus treatment induced physical absorption of covering materials on colloid surface with nonsignificant increases in soil particle size (P > 0.05). These increased the amount of variable functional groups (O-H stretching and bending, C-H stretching, C=O stretching, etc.) by 3-26% and the crystallinity of variable soil minerals (kaolinite, hydromica, and quartz) by 40-300%. However, the fungus treatment of saline-alkali soil obviously differed from the dark brown forest soil. There were 12-35% decreases in most functional groups, 15-55% decreases in crystallinity of most soil minerals but general increases in their grain size, and significant increases in soil particle size (P < 0.05). These different responses sharply decreased element ratios (C: O, C: N, and C: Si) in soil colloids from saline-alkali soil, moving them close to those of the good loam of dark brown forest soil. © 2013 Yanhong Li et al. Source


Zhang L.,Northeast Forestry University | Zhang L.,Key Laboratory of Forest Plant Ecology | Gai Q.-H.,Northeast Forestry University | Gai Q.-H.,Key Laboratory of Forest Plant Ecology | And 8 more authors.
Chinese Journal of Natural Medicines | Year: 2014

Aim: To establish a method to simultaneously determine the main five alkaloids of Catharanthus roseus for trace samples, a high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS) analysis method was developed. Method: The five Catharanthus alkaloids, vinblastine, vincristine, vinleurosine, vindoline, and catharanthine were chromatographically separated on a C18 HPLC column. The mobile phase was methanol-15 nmol·L-1 ammonium acetate containing 0.02% formic acid (65 : 35, V/V). The quantification of these alkaloids was based on the Multiple Reaction Monitoring (MRM) mode. Results: This method was validated, and the results achieved the aims of the study. The intra- and inter-day precision and accuracy of the five alkaloids were within 1.2%-11.5% (RSD%) and -10.9%-10.5% (RE%). The recovery rates of the five alkaloids of samples were from 79.9% to 91.5%. The five analytes were stable at room temperature for 2 h, at 4 °C for 12 h, and at -20 °C for two weeks. The developed method was applied successfully to determine the content of the five alkaloids in three plant parts of three batches of C. roseus with a minute amount collected from three regions of China. Conclusion: The HPLC-ESI-MS/MS method can be used for the simultaneous determination of five important alkaloids in trace C. roseus samples. © 2014 China Pharmaceutical University. Source

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