Key Laboratory of Forensic Genetics

Beijing, China

Key Laboratory of Forensic Genetics

Beijing, China
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Feng L.,Institute of Forensic Science | Feng L.,Key Laboratory of Forensic Genetics | Xu C.,Chinese People's Public Security University | Zeng X.,Public Security Bureau of Fujian Province | And 10 more authors.
Croatian Medical Journal | Year: 2014

Aim: To obtain individual Y-short tandem repeat (STR) profiles in a multi-suspect sexual assault case. Methods We used laser cut microdissection to capture the single sperm cell in the multi-contributor semen sample, combined with the low volume polymerase chain reaction (LV-PCR) method to genotype the single sperm cell profiles using the Yfiler® kit. Consensus DNA profiles were generated from 5 replicate experiments. Results: Ninety-four parallel LV-PCRs were performed and 41 reactions (44%) produced Y-STR profiles with more than nine loci. Three individual Y-STR profiles were successfully obtained. Conclusion: The three Y haplotype units matched three known perpetrators' genotypes. Our results showed that single sperm cells Y-STR analysis was a powerful method for analyzing multi-donor semen mixture sample.

Li C.-X.,Institute of Forensic Science | Li C.-X.,Key Laboratory of Forensic Genetics | Wang G.-Q.,Institute of Forensic Science | Wang G.-Q.,Key Laboratory of Forensic Genetics | And 7 more authors.
Croatian Medical Journal | Year: 2011

Aim: To isolate mucosal cells of the perpetrator in a sexual assault case from a complex mixture of his mucosal cells and the victim's skin by micromanipulation prior to genomic analysis. Methods: To capture and analyze mucosal cells we used the micromanipulation with on-chip low volume polymerase chain reaction (LV-PCR). Consensus DNA profiles were generated from 5 replicate experiments. Results and conclusions: We validated the use of micromanipulation with on-chip LV-PCR for genomic analysis of complex biological mixtures in a fatal rape case. The perpetrator's mucosal cells were captured from nipple swabs of the victim, and a single-source DNA profile was generated from cell mixtures. These data suggest that micromanipulation with on-chip LV-PCR is an effective forensic tool for the analysis of specific cells from complex samples.

Han J.-P.,Chinese People's Public Security University | Li C.-X.,Institute of Forensic Science | Li C.-X.,Key laboratory of Forensic Genetics | Yan H.,Chinese People's Public Security University | And 4 more authors.
Journal of Forensic Medicine | Year: 2012

Objective: To establish optimal amplification conditions with the application of 0.2 mL test tube in single cell separation and inspection. Methods: Oral epithelium cell suspension was prepared. Five or ten cells were collected with 0.2 mL test tube. Then DNA were amplified with Identifiler® Plus kit in three different conditions in which the proteinase K addition, gold enzyme concentration in PCR reaction, and PCR reaction cycles were adjusted separately. Finally the detection rate, allelic dropout rate and artificial alleles were compared among the groups. Results: In these 3 different conditions: addition of proteinase K, addition of 0.4 μL gold enzyme in PCR reaction, and use of 32 cycles, the detection rate was higher and allelic dropout rate was lower than the other conditions. Conclusion: In single cell separation and inspection, the usage of 0.2 mL test tube could be an effective supplement to chip-low volume amplification. © 2012 by the Editorial Department of Journal of Forensic Medicine.

Li C.-x.,Institute of Forensic Science | Li C.-x.,Key Laboratory of Forensic Genetics | Han J.-p.,Chinese People's Public Security University | Ren W.-y.,Chinese People's Public Security University | And 6 more authors.
PLoS ONE | Year: 2011

Genetic profiling of sperm from complex biological mixtures such as sexual assault casework samples requires isolation of a pure sperm population and the ability to analyze low abundant samples. Current standard procedure for sperm isolation includes preferential lysis of epithelial contaminants followed by collection of intact sperm by centrifugation. While effective for samples where sperm are abundant, this method is less effective when samples contain few spermatozoa. Laser capture microdissection (LCM) is a proven method for the isolation of cells biological mixtures, even when found in low abundance. Here, we demonstrate the efficacy of LCM coupled with on-chip low volume PCR (LV-PCR) for the isolation and genotyping of low abundance sperm samples. Our results indicate that this method can obtain complete profiles (13-16 loci) from as few as 15 sperm cells with 80% reproducibility, whereas at least 40 sperm cells are required to profile 13-16 loci by standard 'in-tube' PCR. Further, LCM and LV-PCR of a sexual assault casework sample generated a DNA genotype that was consistent with that of the suspect. This method was unable, however, to analyze a casework sample from a gang rape case in which two or more sperm contributors were in a mixed population. The results indicate that LCM and LV-PCR is sensitive and effective for genotyping sperm from sperm/epithelial cell mixtures when epithelial lysis may be insufficient due to low abundance of sperm; LCM and LV-PCR, however, failed in a casework sample when spermatozoa from multiple donors was present, indicating that further study is necessitated. © 2011 Li et al.

Wei Y.-L.,Xi'an Jiaotong University | Li C.-X.,Institute of Forensic Science | Li C.-X.,Key Laboratory of Forensic Genetics | Li S.-B.,Xi'an Jiaotong University | And 4 more authors.
Behavioral and Brain Functions | Year: 2011

Background: Monoamine oxidases (MAOs) catalyze the metabolism of dopaminergic neurotransmitters. Polymorphisms of isoforms MAOA and MAOB have been implicated in the etiology of mental disorders such as schizophrenia. Association studies detected these polymorphisms in several populations, however the data have not been conclusive to date. Here, we investigated the association of MAOA and MAOB polymorphisms with schizophrenia in a Han Chinese population.Methods: Two functional single nucleotide polymorphisms (SNPs), rs6323 of MAOA and rs1799836 of MAOB, were selected for association analysis in 537 unrelated schizophrenia patients and 536 healthy controls. Single-locus and Haplotype associations were calculated.Results: No differences were found in the allelic distribution of rs6323. The G allele of rs1799836 was identified as a risk factor in the development of schizophrenia (P = 0.00001). The risk haplotype rs6323T-rs1799836G was associated with schizophrenia in female patients (P = 0.0002), but the frequency difference was not significant among male groups.Conclusions: Our results suggest that MAOB is a susceptibility gene for schizophrenia. In contrast, no significant associations were observed for the MAOA functional polymorphism with schizophrenia in Han Chinese. These data support further investigation of the role of MAO genes in schizophrenia. © 2011 Wei et al; licensee BioMed Central Ltd.

Wei Y.-L.,Xi'an Jiaotong University | Li C.-X.,Institute of Forensic Science | Li C.-X.,Key Laboratory of Forensic Genetics | Jia J.,Chongqing Medical University | And 4 more authors.
Journal of Forensic Sciences | Year: 2012

As a powerful alternative to short tandem repeat (STR) profiling, we have developed a novel panel of 47 single nucleotide polymorphisms (SNPs) for DNA profiling and ABO genotyping. We selected 42 of the 47 SNPs from a panel of 86 markers that were previously validated as universal individual identification markers and identified five additional SNPs including one gender marker and four ABO loci. Match probability of the 42 validated SNPs was found to be 9.5×10-18 in Han Chinese. SNP analysis correctly assessed a panel of historical cases, including both paternity identifications in trios and individual identifications. In addition, while STR profiling of degraded DNA provided information for 11 loci of 16 potential markers with low peak intensities, SNPstream® genotyping was sufficient to identify all 47 SNPs. In summary, SNP analysis is equally effective as STR profiling, but appears more suited for individual identification than STR profiling in cases where DNA may be degraded. © 2012 American Academy of Forensic Sciences.

Zhou R.,Institute of Forensic Science | Meng Q.,Institute of Forensic Science | Meng Q.,Key Laboratory of Forensic Genetics | Li P.,Institute of Forensic Science | And 3 more authors.
Australian Journal of Forensic Sciences | Year: 2016

We report the design of a 96-well centrifugal filtration plate and its use in an automated DNA extraction method of touched objects. Cells on the touched objects such as glove prints, swabs from door handles, tools, and so on, are lysed in the 96-well filtration plate. The cell lysate is separated from the objects by centrifugation. Then automated DNA extraction and purification are carried out automatically on a robotic workstation. Such a method not only improves the DNA profile obtained from touched objects but also shortens sample processing time. Processing of 92 samples can be completed in 90 min. © 2015 Australian Academy of Forensic Sciences.

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