Key Laboratory of Food Safety Monitoring and Management of Ministry of Agriculture

Nanjing, China

Key Laboratory of Food Safety Monitoring and Management of Ministry of Agriculture

Nanjing, China
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Zhang C.-Z.,Key Laboratory of Food Safety Monitoring and Management of Ministry of Agriculture | Yang C.-L.,Nanjing Agricultural University | Liu X.-J.,Key Laboratory of Food Safety Monitoring and Management of Ministry of Agriculture | Chen M.,Nanjing Agricultural University | Yu J.,Key Laboratory of Food Safety Monitoring and Management of Ministry of Agriculture
Fenxi Huaxue/ Chinese Journal of Analytical Chemistry | Year: 2011

A sensitive indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) was developed for the detection of new herbicide H-9201, and the performance of ELISA for real sample of environmental water test was evaluated. Starting with phosphorus thiochloride and methanol, an analog with similar structure of hapten was synthesized, and carboxyl group was introduced by reacting with succinic anhydride, then the derivative was conjugated with bovine serum albumin or ovalbumin by means of active ester as immunogen or coating antigen, respectively. IC-ELISA method for H-9201was developed based on the polyclonal antibody generated in rabbits by immunizing with synthesized hapten, with a linear range of 0.05-20 mg L-1. For standard, 50% reduction of the maximum ELISA signal (IC50) was 1.331 mg L-1, whereas the detection limit (IC10) was 0.017 mg L-1. The concentrations of coating solution and primary antibody were optimized to be 1/512 and 1/18000, respectively. No significant cross-reactivity for related compounds, such as DMNT, p-nitrophenol, Methamidophos, Phorate, Chlorpyrifos, and Parathion, was observed. The antibody showed negligible cross-reactivity with S-Carboxylethyl-O,O-Dimethyl-phosphorodithioate, dimethoate, and malathion, but exhibited high cross-reactivity with analogs of hapten. Recovery of H-9201 in environmental water was (80.5% ± 4.5%)-(95.2% ± 3.2%) based on the developed IC-ELISA method. This is the first report for demonstration of new herbicide H-9201 in water by IC-ELISA method. Copyright © 2011, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences.


Liang Y.,Key Laboratory of Food Quality and Safety of Jiangsu Province | Liang Y.,Key Laboratory of Food Safety Monitoring and Management of Ministry of Agriculture | Wang W.,Key Laboratory of Food Quality and Safety of Jiangsu Province | Shen Y.,Key Laboratory of Food Safety Monitoring and Management of Ministry of Agriculture | And 2 more authors.
Food Control | Year: 2012

The dynamics and residue contents of chlorpyrifos and dichlorvos in cucumber grown in greenhouse were investigated. The pesticides were applied at cucumber with different doses and spraying times. Analysis was carried out by liquid chromatography-tandem mass spectrometry. The degradations of chlorpyrifos and dichlorvos applied at 1.5 times recommended dose and 1 spraying times in cucumber coincided with C t = 0.0753e -0.433t, C t = 0.235e -0.199t, respectively. The half-lives obtained were 1.60 days for chlorpyrifos and 3.48 days for dichlorvos. When the cucumber treated with pesticides at the recommended doses and spraying times, the residues of chlorpyrifos was 0.036 mg kg -1 with 1 days interval which below the MRL established by EU, UK and Japan, dichlorvos was 0.008 mg kg -1 with 5 days interval which below the MRL established by EU and UK. When cucumber treated with pesticides at 1.5 times recommended dose or/and one more recommended spraying times, the residues of chlorpyrifos and dichlorvos were below MRLs with 5 or more days interval. Compared with 1.5 times recommended dose, the residue contents of chlorpyrifos and dichlorvos influenced more by one more recommended spraying times. © 2012 Elsevier Ltd.


Liang Y.,Key Laboratory of Food Quality and Safety of Jiangsu Province | Liang Y.,Key Laboratory of Food Safety Monitoring and Management of Ministry of Agriculture | Wang W.,Key Laboratory of Food Quality and Safety of Jiangsu Province | Shen Y.,Key Laboratory of Food Safety Monitoring and Management of Ministry of Agriculture | And 2 more authors.
Food Chemistry | Year: 2012

The effects of washing with tap water and different detergent solutions, storage at different temperatures and ultrasonic cleaning on organophosphorus pesticide (trichlorfon, dimethoate, dichlorvos, fenitrothion, and chlorpyrifos) residue levels in raw cucumber was investigated. Analysis was carried out by liquid chromatography-tandem mass spectrometry. Washing with detergent solutions proved more effective than tap water. The organophosphorus pesticides reduced from 31.1% to 98.8% after washing with detergent solutions for 20 min. Among detergent solutions, 5% sodium carbonate solution caused the greatest loss in trichlorfon and dimethoate, and 5% sodium bicarbonate solution caused the greatest loss in dichlorvos, fenitrothion and chlorpyrifos. Storage at 4°C for 48 h caused pesticides reduction by 60.9-90.2%. Ultrasonic cleaning for 20 min lowered pesticides by 49.8-84.4%. The data indicated that home preparation is effective for the reduction of organophosphorus pesticide residues in raw cucumber and it is useful for reducing the dietary exposure. © 2012 Elsevier Ltd. All rights reserved.

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