Entity

Time filter

Source Type


Wang C.,Jiangsu Academy of Agricultural Sciences | Wang C.,Key Laboratory of Food Safety and Quality of Jiangsu Province | Wang C.,Key Laboratory of Food Safety and Management | Zhang J.,Guabu High School in Jiangsu Province | And 9 more authors.
Tropical Plant Pathology | Year: 2010

The antifungal properties of eugenol were tested against ten plant pathogenic fungal species and detailed studies were conducted regarding in vitro activity of eugenol on Botrytis cinerea. The EC50 value of eugenol on mycelial radial growth of B. cinerea was 38.6 μg/ mL; however, eugenol had no bioactivity against conidia germination. B. cinerea hyphae treated with eugenol showed strong propidium iodide fuorescence in the cytosol. Eugenol increased the concentration of potassium ion and cellular materials in the medium. Furthermore, light and scanning electron microscopy observations on hyphae exposed to eugenol revealed considerable morphological alterations in hyphae, such as cytoplasmic coagulation, vacuolation, and hyphal shriveling. Eugenol induced the generation of H2O2 and increased free Ca2+ in the cytoplasm. These results strongly support the idea that the antifungal activity of eugenol is due to membrane binding and permeability alteration, leading to destabilization and disruption of the plasma membrane. Source


Wang C.-M.,Jiangsu Academy of Agricultural Sciences | Wang C.-M.,Key Laboratory of Food Safety and Quality of Jiangsu Province | Wang C.-M.,Key Laboratory of Food Safety and Management | Guan W.,Jiangsu Academy of Agricultural Sciences | And 18 more authors.
Journal of Asian Natural Products Research | Year: 2010

JS-B (C12H10O3) is a derivative compound of osthol. The antifungal properties of JS-B were tested against 10 economically important plant pathogens. JS-B was effective in inhibiting the mycelial growth of Phytophthora capsici, and its inhibition on different stages of the life cycle of P. capsici was observed. The 50% effective concentration (EC 50) of JS-B on mycelial dry weight and zoospore germination of P. capsici was 43.74 and 86.03g/ml, respectively. The rupture of released zoospores induced by JS-B was reduced by the addition of 100mM glucose. The ultrastructural study showed that JS-B caused destruction of most of the mitochondrions, the concentration of cell nuclear, and the existing vesicles. When compared with dimethomorph, the activity of JS-B on P. capsici was determined under pot conditions. The result showed that JS-B has a curative effect on pepper blight. © 2010 Taylor & Francis. Source


Zhang Z.,Key Laboratory of Food Safety and Quality of Jiangsu Province | Shan W.,Chinese Institute for the Control of Agrochemicals | Jian Q.,Chinese Institute for the Control of Agrochemicals | Song W.,Chinese Institute for the Control of Agrochemicals | And 2 more authors.
Pest Management Science | Year: 2014

Background: Meptyldinocap (2,4-DNOPC) is a novel powdery mildew (Erysiphe necator) fungicide which shows protectant and post-infective activities. However, there is no reliable validated LC-MS/MS method for measuring trace levels of meptyldinocap in cucumber. A method for the determination of 2,4-DNOPC using LC-MS/MS in cucumber and soil was developed. Additionally, the mode of degradation and the residue levels in cucumbers and soils were investigated. Results: Recoveries of meptyldinocap from cucumber and soil samples were found to be 81.4-95.1%. The limits of quantification were found to be 0.01mgkg-1 in cucumber and soil. The half-lives of metpyldinocap in cucumbers and soils were in the ranges of 1.6 to 2.2days and 3.1 to 4.4days. The harvest residue levels of meptyldinocap in cucumbers and soils were in the ranges of below 0.01mgkg-1 to 0.92mgkg-1, and below 0.01mgkg-1 to 0.53mgkg-1. 5days after application; the harvest residue levels of meptyldinocap in cucumbers in all treatments were below 0.1mgkg-1. Conclusion: Compared with the MRL for meptyldinocap in cucumber (0.1mgkg-1), set by the EU, it is suggested that there should be a pre-harvest interval of 5days at the recommended rate and times before harvest of cucumber.© 2013 Society of Chemical Industry. Source


Zhang Z.,Key Laboratory of Food Safety and Quality of Jiangsu Province | Gong Y.,Chinese Institute for the Control of Agrochemicals | Shan W.,Chinese Institute for the Control of Agrochemicals | Jian Q.,Chinese Institute for the Control of Agrochemicals | And 2 more authors.
Chinese Journal of Chromatography (Se Pu) | Year: 2012

A method was developed for the determination of thiophanate-methyl and carbendazim residues in cucumber and soil by using QuEChERS-high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS). The residues in the samples were extracted by acetonitrile, cleaned up by developed QuEChERS method; and then analyzed by using LC-MS/MS in multiple reaction monitoring (MRM) mode via positive electrospray ionization with an Agilent ZORBAX SB-Clg column (30 mm x 2. 1 mm 5, jxm) as the analytical column. The recoveries of thiophanate-methyl spiked at four levels of 0. 01, 0. 05, 0. 1 and 1. 0 mg/kg were from 87. 3% to 96. 0% with the relative standard deviations (RSDs) of 8. 0% -9. 3% in cucumber, from 88. 8% to 93. 4% with the RSDs of 5. 3% -9. 9% in soilj the recoveries of carbendazim spiked at the same levels as those of thiophanate-methyl were from 87. 1% to 92. 3% with the RSDs of 5. 2% -7. 5% in cucumber, from 85. 8% to 90. 9% with the RSDs of 5. 3% -13. 2% in soil. The method is simple; rapid and characterized with acceptable sensitivity and accuracy to meet the requirements of the pesticide residue analysis. This method is applicable to confirm the residues of thiophanate-methyl and carbendazim in cucumber and soil. Source


Zhang Z.,Key Laboratory of Food Safety and Quality of Jiangsu Province | Shan W.,Chinese Institute for the Control of Agrochemicals | Jian Q.,Chinese Institute for the Control of Agrochemicals | Duan L.,Chinese Institute for the Control of Agrochemicals | And 2 more authors.
Chinese Journal of Chromatography (Se Pu) | Year: 2012

A method was developed for the determination of the residues of meptyldinocap (2,4-dinitro-6-(1-methylheptyl) phenyl crotonate, 2,4-DNOPC) as 2, 4-dinitrooctylphenol (2,4-dinitro-6-(1-methylheptyl) phenol, 2,4-DNOP), which is a hydrolysate of 2,4-DNOPC, in six vegetable and fruit by using liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). The residues of 2,4-DNOPC in vegetable and fruit samples were extracted by the mixture of acetone, methanol and hydrochloric acid, then extracted by liquid-liquid partitioning, hydrolyzed under alkaline condition with ultrasonication, and extracted again by liquid-liquid partitioning, then analyzed by using LC-MS/MS in multiple reaction monitoring (MRM) mode via negative electrospray ionization with an Agilent ZORBAX SB-C18 column. The method was validated at four fortification levels in vegetable and fruit. The validation results were as follows: the recoveries of 2,4-DNOPC in cabbages were from 89.7% to 93.3% (the relative standard deviations (RSD) of 6.3% -8.5%), in cucumbers from 87.7% to 95.1% (RSD of 5.8% -10.4%), in tomatoes from 89.3% to 96.0% (RSD of 6.8% -9.2%), in apples from 92.0% to 98.3% (RSD of 5.1% -10.3%), in pears from 89.0% to 95.0% (RSD of 5.3% - 10.2%), in grapes from 81.2% to 95.8% (RSD of 5.8% - 10.4%). The limits of quantification of 2,4-DNOPC in all the test samples were 0.01 mg/kg. The results showed that the method is simple, rapid, and is characterized with acceptable sensitivity and accuracy to meet the requirements of the pesticide residue analysis. This method is applicable to confirm the residues of meptyldinocap in vegetable and fruit samples. Source

Discover hidden collaborations