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Wang C.,Jiangsu Academy of Agricultural Sciences | Wang C.,Key Laboratory of Food Safety and Quality of Jiangsu Province | Wang C.,Key Laboratory of Food Safety and Management | Zhang J.,Guabu High School in Jiangsu Province | And 9 more authors.
Tropical Plant Pathology | Year: 2010

The antifungal properties of eugenol were tested against ten plant pathogenic fungal species and detailed studies were conducted regarding in vitro activity of eugenol on Botrytis cinerea. The EC50 value of eugenol on mycelial radial growth of B. cinerea was 38.6 μg/ mL; however, eugenol had no bioactivity against conidia germination. B. cinerea hyphae treated with eugenol showed strong propidium iodide fuorescence in the cytosol. Eugenol increased the concentration of potassium ion and cellular materials in the medium. Furthermore, light and scanning electron microscopy observations on hyphae exposed to eugenol revealed considerable morphological alterations in hyphae, such as cytoplasmic coagulation, vacuolation, and hyphal shriveling. Eugenol induced the generation of H2O2 and increased free Ca2+ in the cytoplasm. These results strongly support the idea that the antifungal activity of eugenol is due to membrane binding and permeability alteration, leading to destabilization and disruption of the plasma membrane.

Wang C.-M.,Jiangsu Academy of Agricultural Sciences | Wang C.-M.,Key Laboratory of Food Safety and Quality of Jiangsu Province | Wang C.-M.,Key Laboratory of Food Safety and Management | Guan W.,Jiangsu Academy of Agricultural Sciences | And 18 more authors.
Journal of Asian Natural Products Research | Year: 2010

JS-B (C12H10O3) is a derivative compound of osthol. The antifungal properties of JS-B were tested against 10 economically important plant pathogens. JS-B was effective in inhibiting the mycelial growth of Phytophthora capsici, and its inhibition on different stages of the life cycle of P. capsici was observed. The 50% effective concentration (EC 50) of JS-B on mycelial dry weight and zoospore germination of P. capsici was 43.74 and 86.03g/ml, respectively. The rupture of released zoospores induced by JS-B was reduced by the addition of 100mM glucose. The ultrastructural study showed that JS-B caused destruction of most of the mitochondrions, the concentration of cell nuclear, and the existing vesicles. When compared with dimethomorph, the activity of JS-B on P. capsici was determined under pot conditions. The result showed that JS-B has a curative effect on pepper blight. © 2010 Taylor & Francis.

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