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Huang W.,Sun Yat Sen University | Zhang Y.,Sun Yat Sen University | Jia X.,Key Laboratory of Fishery Ecology Environment | Ma X.,Sun Yat Sen University | And 13 more authors.
Fish Physiology and Biochemistry | Year: 2010

Environmental estrogens, such as bisphenol A (BisA) and nonylphenol (NP), have been shown to affect the estrogen receptor (ER) expression and induce male reproductive abnormalities. To elucidate molecular mechanisms of action of xenoestrogenic chemicals on the expression of estrogen receptors in the testes of Nile tilapia (Oreochromis niloticus), three full-length cDNAs respectively encoding ntERα, ntERβ1 and ntERβ2 were cloned from testes. The amino acid sequences of ntERα, ntERβ1 and ntERβ2 showed a high degree of similarity to the relevant fish species. Tissue-specific expression study showed that three receptors were highly expressed in pituitary, liver, testis, kidney and intestine tissues. The ntERα, ntERβ1 and ntERβ2 mRNA expressions were significantly higher at the sexual early recrudescing stage than at other recrudesced stages. After being exposed to xenoestrogens from weeks 2 to 4, the ntERα mRNA levels were increased significantly in testes after NP treatment at all sampling times or after 4 weeks of exposure to BPA. The ntERβ1 mRNA levels remained unchanged, while a significant decrease of the ntERβ2 mRNA level was observed in testes after exposure to NP and BPA. The present study demonstrates that the regulation of all three ntER subtypes in testes may act via different molecular mechanisms of exposure to NP and BPA. © 2008 Springer Science+Business Media B.V.

Wang Z.-H.,CAS South China Sea Fisheries Research Institute | Wang Z.-H.,Key Laboratory of Fishery Ecology Environment | Wang Z.-H.,Key Laboratory of Aquatic Product Processing | Wang X.-N.,CAS South China Sea Fisheries Research Institute | And 2 more authors.
Yejin Fenxi/Metallurgical Analysis | Year: 2014

An atomic absorption spectrometric method for the determination of copper, lead, cadmium, zinc, chromium and nickel in seawater was established. Under the conditions of pH 4.0±0.5 and heating in a water bath, with ammonium pyrrolidine dithiocarbamate (APDC) as a chelating agent, copper, lead, cadmium, zinc, chromium and nickel were chelated quantitatively to form chelates which were extracted by MIBK, then zinc was determined by flame atomic absorption spectrometry and the other elements were determined by graphite furnace atomic absorption spectrometry. The results showed that the detection limits for cadmium, chromium, copper, lead, nickel and zinc were 0.04, 0.3, 0.13, 0.2, 0.4 and 1.0 g/L, respectively. Certified reference seawater samples GBW (E) 080040 was analyzed, except that nickel had no certified value, the measured results of the other elements were consistent with the certified values. The method was applied to the analysis of seawater from the Pearl River estuary and the Daya Bay, with the relative standard deviations (RSD, n=7) for the measurement results of each element between 2.3% to 6.5% and the recoveries in the range of 94%-105%.

Gu Y.,CAS South China Sea Fisheries Research Institute | Gu Y.,Jinan University | Gu Y.,Key Laboratory of Fishery Ecology Environment | Yang Y.,Jinan University | And 2 more authors.
Shenzhen Daxue Xuebao (Ligong Ban)/Journal of Shenzhen University Science and Engineering | Year: 2014

Surface and core sediments were collected from Baisha Bay, Nan'ao Island, one of Guangdong Province's largest mariculture bases in Southern China. Acid-volatile sulfide (AVS) and simultaneously extracted metals (SEM) including Cd, Pb, Cr, Ni, Cu and Zn were analyzed to evaluate sediment quality. In surface sediments, the contents (dry weight) of AVS and ∑SEM were from 0.04 to 37.62 μmol/g and from 1.54 to 3.82 μmol/g, with average levels of 6.36 and 2.45 μmol/g, respectively. The highest contents of AVS, ∑SEM and molar ratios of AVS to ∑SEM were in fish culture zones. In core sediments, the concentrations (dry weight) of AVS and ∑SEM varied in the range of 0.04-16.27 μmol/g and 1.72-21.14 μmol/g, with average levels of 2.26 μmol/g and 4.71 μmol/g, respectively. The AVS content dramatically increased from bottom to surface sediments in fish culture zone, suggesting that AVS might play a major role in binding heavy metals. On the contrary, the ∑SEM content rapidly decreased from bottom to surface sediments in shellfish culture zone. In the surface sediments, the ration of AVS/∑SEM is larger than 1 in the fish cage, shellfish and seaweed zones, indicating that those heavy metals lightly damaged aquatic organisms.

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