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Qi Z.,Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization | Wang J.,Key Laboratory of Fishery Ecology and Environment | Wang J.,CAS South China Sea Fisheries Research Institute | Mao Y.,Chinese Academy of Fishery Sciences | And 2 more authors.
Journal of the World Aquaculture Society | Year: 2013

Feasibility of offshore co-culture of abalone and sea cucumber was investigated in Northern China. Survival and growth of abalone, Haliotis discus hannai Ino, and sea cucumber, Apostichopus japonicus, co-cultured in abalone cages from suspended longlines, in the offshore area, were examined. Abalone and sea cucumbers were co-cultured at density ratios of both 3:1 and 6:1 for 1yr. Abalone were fed with fresh kelp and no additional feed was given to sea cucumbers. Survival of abalone and sea cucumber was 100% for all treatments. Abalone and sea cucumber grew well; the body weight (BW) of abalone and sea cucumber was nearly doubled and had reached a commercial size. There were no significant differences in the growth rates for both abalone and sea cucumber between the two density treatments. The specific growth rate of BW of abalone (SGRbw) was highest in June, with a value of 0.536%/d. Growth rate of sea cucumber (SGRsc) was highest in December, reached 1.84%/d, with an annual average SGRsc of 0.182%/d. Results suggested that the offshore co-culture of abalone and sea cucumber was feasible offshore. The co-culture of abalone with sea cucumbers may provide an additional valuable crop without additional financial input. © Copyright by the World Aquaculture Society 2013.


Wang R.X.,CAS South China Sea Fisheries Research Institute | Wang R.X.,Key Laboratory of Fishery Ecology and Environment | Wang R.X.,South China Normal University | Wang A.,South China Normal University | And 2 more authors.
Ecotoxicology and Environmental Safety | Year: 2014

A total of 1,050 strains of heterotrophic bacteria isolated from farming seawater and the intestines of oyster species Crassostrea hongkongensis were tested for resistance to 10 antibiotics by the Kirby-Bauer diffusion method. The resistant rates of seawater-derived bacteria to chloramphenicol, enrofloxacin, and ciprofloxacin were low (less than 20%), whereas the bacteria obtained from oysters showed low resistance to chloramphenicol and enrofloxacin. Many strains showed high resistant rates (more than 40%) to furazolidone, penicillin G, and rifampin. A total of 285 strains from farming seawater and oysters were resistant to more than three antibiotics. Several strains showed resistance to more than nine antibiotics. Furthermore, the peak resistant rates of the seawater-derived strains to multiple antibiotics overlapped in April, June, September, and November, and those of oyster-derived strains overlapped during April, July, and September. The multi-resistant rate patterns of strains from farming seawater and oyster intestines were similar. © 2014 Elsevier Inc.


Wang Z.,CAS South China Sea Fisheries Research Institute | Wang Z.,Key Laboratory of Fishery Ecology and Environment | Wang Z.,Key Laboratory of Aquatic Product Processing | Wang X.,CAS South China Sea Fisheries Research Institute | And 3 more authors.
Journal of Applied Phycology | Year: 2014

The macroalga Gracilaria lemaneiformis is an important and commercially valuable renewable resource. It is distributed widely in shallow marine waters but grows mostly on tropical or subtropical coasts. We investigated the accumulation of Cd, Cu, and Pb by live G. lemaneiformis under low concentrations. There was a positive correlation between the organisms' metal concentrations and exposure concentrations. When exposed to both Cu and Cd, the concentrations of Cu and Cd in G. lemaneiformis were higher than those exposed to solutions of Cu and Cd alone. However, the concentrations of heavy metals in G. lemaneiformis were not markedly different (p < 0.05) between the treatment groups and the control groups. We analyzed the results with nonlinear curve fitting and employed a two-compartment model to study the accumulation kinetics of heavy metals by G. lemaneiformis. The uptake rate constants and bioconcentration factors (BCFs) of the metals decreased with increased exposure concentration. The theoretical equilibrium concentrations increased significantly with the exposure concentrations. Our results suggested that G. lemaneiformis obviously accumulated heavy metals from seawater. As an important resource for food and pharmaceuticals, G. lemaneiformis should be cultivated in clear seawater. © 2013 Springer Science+Business Media Dordrecht.


Du F.Y.,CAS South China Sea Fisheries Research Institute | Du F.Y.,Key laboratory of Fishery Ecology and Environment | Wang X.H.,CAS South China Sea Fisheries Research Institute | Wang X.H.,Key laboratory of Fishery Ecology and Environment | And 2 more authors.
Shengtai Xuebao/ Acta Ecologica Sinica | Year: 2015

Zooplankton is an important component of the coral reef ecosystem, but studies on this component are insufficient in China. Based on 13 samples collected in July 2012, the characteristics of zooplankton community in the Meiji coral reef waters were studied. It was found that the reef waters are rich in zooplankton species and a total number of 138 species belonging to 15 taxa was recorded. The dominant species were fish eggs, Oikopleura longicauda, Macrura larvae, Centropages orsinii, Oikopleura sp., Sagitta robusta, Brachyura zoea, and Fritillaria haplostoma. According to habitats of the species, they could be categorized into warm-water coastal fauna and warm-water oceanic fauna. There was no obvious species dominating throughout three types of habitats in the reef waters and the species compositions and dominant species varied with habitats. The dominant species compositions in seaward slope were the most complex and 15 species were recorded. The main dominant species were Oithona plumifera, Thalia democratica and Oikopleura sp. in seaward slope. The similarities of species compositions of seaward slope to lagoon and to reef flat were only 27.8% and 21%, respectively, whereas, the similarity of species compositions between lagoon and reef flat was as high as 77.8%. The reef waters are rich in zooplankton diversities. The average indexes of species richness, diversity and evenness were 4.93, 3.33 and 0.67, respectively, and the overall threshold value of diversity was 2.43. The threshold values of seaward slope, lagoon and reef flat were 4.04, 1.98 and 1.38, respectively, showing that the zooplankton diversity was the highest in the seaward slope waters. The mean abundance and wet biomass of zooplankton were 117.70 ind/ m3 and 69.01 mg/ m3, respectively, and the zooplankton abundance was mainly composed of larvae. The abundance of larvae was 52.32 ind/ m3, taking up 44.5% of total zooplankton abundance. The zooplankton community in the reef waters could be classified into the lagoon-reef flat group and the seaward slope group. The test of One-way ANOSIM showed that compositions of the two groups were significantly different (R = 0.865, P = 0.001). Due to topographical heterogeneity and differences in hydrodynamic conditions in the reef waters, the regional variation of the zooplankton community was obvious. Being the low wave energy zone, the hydrodynamic changes, winds and waves of the lagoon is the least. In the lagoon waters, the zooplankton diversity was higher, but the biomass was the lowest. Whereas in reef flat, due to medium wave energy and moderate hydrodynamic changes, the zooplankton abundance and biomass was higher, with uneven distributions, and the diversity was lower. Adjoining the open ocean, seaward slope is steep in topography, drastic change in water depth, and upwelling can occur there. The diversified environment conditions are fit for the growth of a variety of zooplankton species. Therefore, the species richness and diversity of zooplankton in the seaward slope was the highest. However, located in the high wave energy zone, the hydrodynamic and water turbulence in the seaward slope are the strongest. The conditions do not favor zooplankton growth and the zooplankton abundance there was the lowest. © 2015, Ecological Society of China. All rights reserved.


He H.,South China Agricultural University | Yu J.,Key Laboratory of Fishery Ecology and Environment | Chen G.,South China Agricultural University | Li W.,South China Agricultural University | And 2 more authors.
Ecotoxicology and Environmental Safety | Year: 2012

Both single and joint toxicity of atrazine and butachlor to freshwater green alga Scenedesmus obliquus and cladoceran Daphnia carinata isolated from South China were investigated in the present study. The 96h-EC 50 values of atrazine and butachlor to S. obliquus were 0.0147 and 2.31mgL -1, while the 48h-LC 50 values to D. carinata were 60.6 and 3.40mgL -1, respectively. These results suggest that atrazine could be highly toxic to S. obliquus and slightly toxic to D. carinata, while butachlor exhibits moderate toxicity to both organisms. The additive indexes of atrazine and butachlor mixtures were -2.68 (-3.02 to -2.32) to S. obliquus and 0.054 (-0.025 to 0.238) to D. carinata, respectively. Therefore, the joint action of two herbicides was significant antagonism to S. obliquus, while significant synergism was not shown to D. carinata. Moreover, significant linear correlation between the natural logarithm of herbicide concentrations and growth rates of alga S. obliquus was observed. Taken together, it is the first study reporting the toxicity endpoints for mixture of atrazine and butachlor against S. obliquus and D. carinata isolated from south China. The present results would be helpful to provide data to assess the ecological risk of both herbicides to aquatic organisms. © 2012 Elsevier Inc.


Wang R.X.,South China Normal University | Wang R.X.,CAS South China Sea Fisheries Research Institute | Wang R.X.,Key Laboratory of Fishery Ecology and Environment | Wang J.Y.,CAS South China Sea Fisheries Research Institute | And 4 more authors.
Marine Pollution Bulletin | Year: 2015

546 Vibrio isolates from rearing seawater (292 strains) and intestines of abalone (254 strains) were tested to ten antibiotics using Kirby-Bauer diffusion method. Resistant rates of abalone-derived Vibrio isolates to chloramphenicol (C), enrofloxacin (ENX) and norfloxacin (NOR) were <. 28%, whereas those from seawater showed large fluctuations in resistance to each of the tested antibiotics. Many strains showed higher resistant rates (>. 40%) to kanamycin (KNA), furazolidone (F), tetracycline (TE), gentamicin (GM) and rifampin (RA). 332 isolates from seawater (n. =. 258) and abalone (n. =. 74) were resistant to more than three antibiotics. Peaked resistant rates of seawater-derived isolates to multiple antibiotics were overlapped in May and August. Statistical analysis showed that pH had an important effect on resistant rates of abalone-derived Vibrio isolates to RA, NOR, and ENX. Salinity and dissolved oxygen were negatively correlated with resistant rates of seawater-derived Vibrio isolates to KNA, RA, and PG. © 2015 Elsevier Ltd.


Zhang L.,Chinese Academy of Sciences | Zhang L.,CAS Yantai Institute of Coastal Zone Research | Zhang L.,University of Chinese Academy of Sciences | Gan J.,Key Laboratory of Fishery Ecology and Environment | And 8 more authors.
Environmental Toxicology and Pharmacology | Year: 2012

With the objective to identify promising molecular biomarkers for marine pollution monitoring, a new cytochrome P450 gene was identified from Venerupis (Ruditapes) philippinarum and classified as a member of a new subfamily, CYP414A1. Phylogenetic analysis showed that CYP414A1 was closely related to members of the CYP2 family. CYP414A1 mRNA expression was significantly induced by 50μg/L B[. a]P at 96. h, while no significant change was found in 5μg/L B[. a]P-exposed samples. For heavy metals exposure, the expression of CYP414A1 was significantly up-regulated by Cd but sharply depressed by Cu exposure. These results suggested that CYP414A1 responded to various xenobiotics stresses, and could be used as a candidate biomarker of heavy metals and B[a]P. © 2011 Elsevier B.V.


Wang R.,CAS South China Sea Fisheries Research Institute | Wang R.,Key Laboratory of Fishery Ecology and Environment | Wang R.,Key Laboratory of Aquatic Products Processing | He J.,CAS South China Sea Fisheries Research Institute | And 2 more authors.
Journal of Shellfish Research | Year: 2016

Currently, the knowledge of ecology and function of bacteria harbored by molluscs is very limited. In this study, quantity and richness of culturable heterotrophic bacteria (CHB) in seawater of the farming environment and guts of the oyster Crassostrea hongkongensis were determined monthly, with classical, biochemical, and molecular methods. About 800 randomly selected bacterial strains were purified and identified.Quantities of CHB and vibrios, ranging from2.3×103 to 2.2×105 CFU/ml and 1.6×102 to 3.0×103 CFU/ml, respectively, were present in the farming seawater, whereas those in the oyster C. hongkongensis ranged from 5.3×104 to 5.4×106 CFU/g and 6.0×103 to 1.4×105 CFU/g, respectively. Vibrio spp., Pseudoalteromonas spp., Bacillus spp., and Shewanella spp. appeared almost every month. Richness of heterotrophic microbial communities of the seawater and the oysters was very similar and peaked in August. Variation in the diversity (Shannon index) of CHB in the seawater and in the oysters was similar, a significant positive correlation was showed between them (r = 0.894), and their diversities were both peaked in August. Statistical analysis showed that salinity hasmore important impact on quantity of CHB in seawater. Similarly, temperature hadmore important impact on that in the oysters. Important threshold data were analyzed to further explain the relationships.


Gu Y.-G.,CAS South China Sea Fisheries Research Institute | Gu Y.-G.,Key Laboratory of Fishery Ecology and Environment | Gu Y.-G.,Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization | Lin Q.,CAS South China Sea Fisheries Research Institute | And 4 more authors.
Marine Pollution Bulletin | Year: 2014

Surface sediments collected from Zhelin Bay, the largest mariculture base of eastern Guangdong Province, were analyzed for total metal concentrations and chemical speciation. The results demonstrated that the average total concentration (mg/kg) ranges were 36.7-65.8 (Pb), 53.8-98.8 (Cr), 39.0-87.1 (Ni), 50.9-144.5 (Cu), and 175.0-251.2 (Zn), which were clearly higher with respect to their corresponding benchmark values. The predominant speciation of Pb was reducible and comprised a residual fraction, whereas a major portion (57.6-95.4%) of Cr, Ni, Cu, and Zn was strongly associated with the residual fractions. Taking as a whole, surface sediments of Zhelin Bay had a 21% probability of toxicity based on the mean effects range-median quotient. © 2014 Elsevier Ltd.


PubMed | Key Laboratory of Fishery Ecology and Environment
Type: Journal Article | Journal: World journal of microbiology & biotechnology | Year: 2012

Four marine bacterial strains P1, P5, N5 and N21 were isolated from the surface water and sediment of Mirs Bay in southern Chinese coast using the liquid infection method with 48-well plates. These bacteria were all shown to have algicidal activities against Skeletonema costatum. Based on morphological observations, biochemical tests and homology comparisons by 16S rDNA sequences, the isolated strains P1, P5, N5 and N21 were identified as Halobacillus sp., Muricauda sp., Kangiella sp. and Roseivirga sp., respectively. Our results showed that bacterial strain P1 killed S. costatum by release of heat labile algicide, while strains P5, N5 and N21 killed them directly. The algicidal processes of four bacterial strains were different. Strains P1, N5 and N21 disrupted the chain structure and S. costatum appeared as single cells, in which the cellular components were aggregated and the individual cells were inflated and finally lysed, while strain P5 decomposed the algal chains directly. We also showed that the algicidal activities of the bacterial strains were concentration-dependent. More specifically, 10 % (v/v) of bacteria in algae showed the strongest algicidal activities, as all S. costatum cells were killed by strains N5 and N21 within 72 h and by strains P1 and P5 within 96 h. 5 % of bacteria in algae also showed significant algicidal activities, as all S. costatum were killed by strains N5, P5 and N21 within 72, 96 and 120 h, respectively, whereas at this concentration, only 73.4 % of S. costatum cells exposed to strain P1 were killed within 120 h. At the concentration of 1 % bacteria in algae, the number of S. costatum cells continued to increase and the growth rate of algae upon exposure to strain N5 was significantly inhibited.

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