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Wang H.-B.,Ningxia Medical University | Li Y.-X.,Ningxia Medical University | Hao Y.-J.,Ningxia Medical University | Wang T.-F.,Ningxia Medical University | And 14 more authors.
European Review for Medical and Pharmacological Sciences | Year: 2013

AIM: The present study was conducted to investigate whether LBP had a protective effect on cerebral ischemic reperfusion injury and to determine the possible mechanisms. MATERIALS AND METHODS: Male Kunming (KM) mice were used to make the model cerebral artery occlusion/reperfusion (MCAO/R). The behavioral test was used to measure neurological deficit scores for evaluation of ischemic reperfusion damage of brain. The change of electroencephalograph (EEG) was monitored by Model SMUP-E Bio-electric Signals Processing System. The infarction area of brain was assessed in brain slices with 2% solution of 2,3,5-triphenyl tetrazolium chloride (TTC). Spectrophotometric assay was used to determine the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) and lactate dehydrogenase (LDH), contents of malondialdehyde (MDA) and adenosine triphosphate (ATP) of the brain. RESULTS: The results showed that LBP at doses of 20 and 40 mg/kg markedly decreased the neurological deficit scores and the infarction area in MCAO/R mice. At the same time, LBP significantly decreased MDA content, and increased SOD, GSH-Px, CAT, LDH activities in ischemic reperfusion brain. CONCLUSIONS: These suggest that LBP might act as a potential neuroprotective agent against the cerebral reperfusion-induced injury in the brain through reducing lipid peroxides, scavenging free radicals, and improving the energy metabolism. Source


Wang H.,Ningxia Medical University | Ma L.,Ningxia Key Laboratory of Craniocerbral Diseases of Ningxia Hui Autonomous Region | Liu J.,Ningxia Medical University | Zhao C.,Ningxia Medical University | And 6 more authors.
Pharmaceutical Biology | Year: 2013

Context: Primary dysmenorrhea is one of the most frequent gynecological disorders in young women. Chinese herbal medicine has the advantage in terms of multi-targeting efficacy, lower toxicity, as well as lower cost. Core licorice is the hard and atropurpureus heart part in root and rootstock of Glycyrrhiza uralensis Fisch (Leguminosae), having a therapeutic effect on dysmenorrhea. Objective: This experiment indicated the spasmolytic effect of core licorice aqueous extract (CLE) on spontaneous rhythmic contractions and spasmogen-provoked contractions of stilbestrol primed, estrogen-dominated, non-pregnant mouse isolated uterine horns and its spasmolytic mechanism. Materials and methods: We investigated the spasmolytic effect of CLE (0.025-0.1mg/mL) on spontaneous contractions and potassium chloride (KCl, 40mM), acetylcholine (ACh, 5μg/mL), carbachol (CCh, 5μg/mL), oxytocin (OT, 2 U/L) or bradykinin (5ng/mL)-provoked contractions of mouse isolated uterine horns. Contractions were recorded by tension force transducers using Biolap 420F software on a PC. Results: Our present study showed that graded, escalated concentrations of CLE (0.025-0.1mg/mL) significantly inhibited the amplitude of spontaneous phasic contractions (15.03-55.10%), as well as the contractions produced by KCl (40mM; 20.16-53.99%), ACh (5μg/mL; 14.65-48.32%), CCh (5μg/mL; 38.40-76.70%), OT (2 U/L; 21.53-58.49%) or bradykinin (5ng/mL; 58.01-79.44%) of the estrogen-dominated isolated mice uterine horn preparations in a concentration-related manner. Discussion and conclusion: The spasmolytic effect of CLE observed in the present study lends pharmacological support to the traditional use of core licorice in the management, control and treatment of primary dysmenorrhea. © 2013 Informa Healthcare USA, Inc. All rights reserved: reproduction in whole or part not permitted. Source


Rui C.,Ningxia Medical University | Yuxiang L.,Ningxia Medical University | Yinju H.,Ningxia Medical University | Qingluan Z.,Ningxia Medical University | And 12 more authors.
Journal of Molecular Histology | Year: 2012

This study investigated the protective effects of Lycium barbarum polysaccharide (LBP) on alleviating injury from oxygen-glucose deprivation/reperfusion (OGD/ RP) in primary cultured rat hippocampal neurons. Cultured hippocampal neurons were exposed to oxygen-glucose deprivation (OGD) for 2 h followed by a 24 h re-oxygenation. The MTT assay and the lactate dehydrogenase (LDH) release were used to determine the neuron viability. Superoxide dismutase (SOD), Glutathione peroxidase (GSH-PX), malondialdehyde (MDA) were determined by spectrophotometry using commercial kits. Mitochondrial membrane potential (MMP) and the intracellular free calcium concentration ([Ca2+]i) in hippocampal neurons were measured using the confocal laser scanning microscope (CLSM). Treatment with LBP (10-40 mg/l) significantly attenuated neuronal damage and inhibited LDH release in a dose-dependent manner. Furthermore, LBP enhanced activities of SOD and GSH-PX but it decreased their MDA content, inhibited [Ca2+]i elevation and decrease of MMP in ischemia-reperfusion treated hippocampal neurons. These findings suggested that LBP may be a potential neuroprotective agent for cerebral ischemia-reperfusion injury. © Springer Science+Business Media B.V. 2012. Source


Haiyan W.,Ningxia Medical University | Yuxiang L.,Ningxia Medical University | Linglu D.,Ningxia Medical University | Tingting X.,Ningxia Medical University | And 6 more authors.
Pharmaceutical Biology | Year: 2013

Context: Sophora alopecuroides L. (Leguminosae) is a commonly used Chinese herbal drug that possesses antipyretic, anti-inflammatory and analgesic effects. Among various alkaloids isolated from S. alopecuroides, matrine has been identified as the major bioactive component contributing to a variety of pharmacological effects, and studies have also shown that matrine has an analgesic effect. Objective: To investigate the antinociceptive effects of matrine on neuropathic pain induced by chronic constriction injury (CCI) in mice. Materials and methods: The von Frey, plantar, cold-plate, locomotor activity and rota-rod test were performed to assess the degree of mechanical, radiant, thermal, spontaneous locomotor activity and motor coordination changes respectively, at different time intervals, i.e., one day before surgery and 7, 8, 10, 12 and 14 days post surgery. Matrine was administered from the 8th day after the surgery for seven days. Results: Our present study shows that matrine at the dose of 30 mg/kg i.p. increased the paw withdrawal threshold (0.88 ± 0.16), paw withdrawal latency (7.01 ± 0.11) and the counts of paw withdrawal (19.7 ± 1.15) from the day 8 for the nerve injured paw compared to the CCI group (0.18 ± 0.04, 4.62 ± 0.18, 44.3 ± 2.99, respectively). Matrine, in a dose-dependent effect, was also found to produce a protective role in both plantar and cold-plate tests. The analysis of the effect supports the hypothesis that matrine is useful in neuropathic pain therapy. Discussion and conclusion: The results of this study suggest that matrine could be useful in the treatment of different kinds of neuropathic pains as an adjuvant to conventional medicines. © 2013 Informa Healthcare USA, Inc. Source


Zhang C.,Key Laboratory of Fertility Preservation and Maintenance | Zhang C.,Key Laboratory of Reproduction | Dang J.,Key Laboratory of Fertility Preservation and Maintenance | Dang J.,Key Laboratory of Reproduction | And 17 more authors.
American Journal of Human Biology | Year: 2013

Objectives: The relative length of the second-to-fourth digits (2D:4D) has been linked with prenatal androgen in humans. A recent study shows that the 2D:4D ratio in mice is controlled by the balance of androgen to estrogen signaling during a narrow window of digit development. Androgen receptor (AR) activity is higher in digit 4 than in digit 2, and inactivation of AR decreases growth of digit 4, which causes a higher 2D:4D ratio. At the molecular level, the effect of androgens is mediated through the activation of AR. The CAG/GGN repeat polymorphisms of the AR gene are associated with AR activity. Here, we investigate the effect of CAG/GGN repeat polymorphisms in AR on 2D:4D in Chinese. Methods: Digit lengths of the second and fourth fingers were measured from photocopies of the ventral surface of the hand and by actual finger measurements. We genotyped AR polymorphisms by ABI 3730 DNA analyzer. Results: We found that left hand 2D:4D ratio was longer than that of the right hand both in males and in females. We failed to find any relationship between CAG/GGN alleles and the left hand, right hand, right minus left-hand or mean hand 2D:4D ratios (all, r < 0.20, P > 0.05). Conclusions: In this study, we first found that the left hand 2D:4D ratio was longer than that of the right hand in both males and females. However, we found that both CAG and GGN alleles were not associated with the left hand, right hand, right minus left-hand or mean hand 2D:4D ratios. © 2012 Wiley Periodicals, Inc. Source

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