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Lu W.,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources | Lu W.,Shanghai Ocean University | Jin Y.,Shanghai Ocean University | Xu J.,Shanghai Ocean University | And 2 more authors.
General and Comparative Endocrinology | Year: 2017

Parathyroid hormone-related protein (PTHrP) is a hypercalcemic factor in fish, but the source of circulating PTHrP remains unclear. In this study investigation of the caudal neurosecretory system (CNSS), considered one of major sources of PTHrP in fish, provided valuable insights into this regulatory system. We report pthrpa and pthrpb gene cloning, characterization, expression, and responses to low salinity and hypocalcemia challenge in flounder. The pthrpa and pthrpb precursors, isolated from a European flounder CNSS library, consist of 166 and 192 amino acid residues, respectively, with an overall homology of approximately 59.2%. Both precursors contain a signal peptide and a mature peptide with cleavage and amidation sites. The flounder PTHrPA and PTHrPB peptides share only 41% sequence identity with human PTHrPA. Quantitative PCR analysis demonstrated that the bone and bladder, are respectively major sites of pthrpa and pthrpb expression in flounder. Urophysectomy confirmed the CNSS as a likely contributor to circulating PTHrP peptides. There were no significant differences in CNSS pthrpa and pthrpb mRNA expression or plasma PTHrP levels between seawater (SW) and freshwater (FW)-adapted fish, though plasma total calcium concentrations were higher in FW animals. The intraperitonial administration of EGTA rapidly induced hypocalcemia and concomitant elevation in plasma PTHrP accompanied by increases in both pthrpa and pthrpb expression in the CNSS. Together, these findings support an evolutionary conserved role for PTHrP in the endocrine regulation of calcium. © 2017 Elsevier Inc.


Hu M.,Shanghai Ocean University | Hu M.,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources | Hu M.,Alfred Wegener Institute for Polar and Marine Research | Wu F.,Shanghai Ocean University | And 8 more authors.
Chemosphere | Year: 2015

Bloom forming algae and hypoxia are considered to be two main co-occurred stressors associated with eutrophication. The aim of this study was to evaluate the interactive effects of harmful algae Microcystis aeruginosa and hypoxia on an ecologically important mussel species inhabiting lakes and reservoirs, the triangle sail mussel Hyriopsis cumingii, which is generally considered as a bio-management tool for eutrophication. A set of antioxidant enzymes involved in immune defence mechanisms and detoxification processes, i.e. glutathione-S-transferases (GST), glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), lysozyme (LZM) in mussel haemolymph were analyzed during 14. days exposure along with 7. days depuration duration period. GST, GSH, SOD, GPX and LZM were elevated by toxic M. aeruginosa exposure, while CAT activities were inhibited by such exposure. Hypoxia influenced the immune mechanisms through the activation of GSH and GPX, and the inhibition of SOD, CAT, and LZM activities. Meanwhile, some interactive effects of M. aeruginosa, hypoxia and time were observed. Independently of the presence or absence of hypoxia, toxic algal exposure generally increased the five tested enzyme activities of haemolymph, except CAT. Although half of microcystin could be eliminated after 7. days depuration, toxic M. aeruginosa or hypoxia exposure history showed some latent effects on most parameters. These results revealed that toxic algae play an important role on haemolymph parameters alterations and its toxic effects could be affected by hypoxia. Although the microcystin depuration rate of H. cumingii is quick, toxic M. aeruginosa and/or hypoxia exposure history influenced its immunological mechanism recovery. © 2015 Elsevier Ltd.


Sui Y.,Shanghai Ocean University | Kong H.,Shanghai Ocean University | Huang X.,Shanghai Ocean University | Dupont S.,Gothenburg University | And 10 more authors.
Chemosphere | Year: 2016

Hypoxia and ocean acidification are two consequences of anthropogenic activities. These global trends occur on top of natural variability. In environments such as estuarine areas, short-term acute pH and O2 fluctuations are occurring simultaneously. The present study tested the combined effects of short-term seawater acidification and hypoxia on the physiology and energy budget of the thick shell mussel Mytilus coruscus. Mussels were exposed for 72 h to six combined treatments with three pH levels (8.1, 7.7 and 7.3) and two dissolved oxygen (DO) levels (2 mg L-1, 6 mg L-1). Clearance rate (CR), food absorption efficiency (AE), respiration rate (RR), ammonium excretion rate (ER), O:N ratio and scope for growth (SFG) were significantly reduced, and faecal organic dry weight ratio (E) was significantly increased at low DO. Low pH did not lead to a reduced SFG. Interactive effects of pH and DO were observed for CR, E and RR. Principal component analysis (PCA) revealed positive relationships among most physiological indicators, especially between SFG and CR under normal DO conditions. These results demonstrate that Mytilus coruscus was sensitive to short-term (72 h) exposure to decreased O2 especially if combined with decreased pH levels. In conclusion, the short-term oxygen and pH variation significantly induced physiological changes of mussels with some interactive effects. © 2016 Elsevier Ltd.


Sui Y.,Shanghai Ocean University | Hu M.,Shanghai Ocean University | Hu M.,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources | Hu M.,Alfred Wegener Institute for Polar and Marine Research | And 6 more authors.
Marine Environmental Research | Year: 2015

Ocean acidification and hypoxia, both caused by anthropogenic activities, have showed deleterious impacts on marine animals. However, their combined effect on the mussel's defence to its predator has been poorly understood, which hinders us to understand the prey-predator interaction in marine environment. The thick shell mussel Mytilus coruscus and its predator, the Asian paddle crab Charybdis japonica were exposed to three pH levels (7.3, 7.7, 8.1) at two concentrations of dissolved oxygen (2.0 mg L-1, 6.0 mg L-1) seawater. The anti-predatory responses of mussels, in terms of byssus thread production were analyzed after 72 h exposure. During the experiment, frequency of shedding stalks (mussels shed their byssal stalks to release themselves from attachment and allow locomotion) and number of byssus threads increased with time, were significantly reduced by hypoxia and low pH levels, and some interactions among time, predator, DO and pH were observed. As expected, the presence of the crab induced an anti-predator response in M. coruscus (significant increases in most tested parameters except the byssus thread length). Acidification and hypoxia significantly reduced byssus thread diamter at the end of the experiment, but not the byssus thread length. Cumulative byssus thread length and volume were significantly impaired by hypoxia and acidification. Our results highlight the significance of anti-predatory responses for adult mussel M. coruscus even under a stressful environment in which stress occurs through ocean acidification and hypoxia. By decreasing the strength of byssus attachment, the chance of being dislodged and consumed by crabs is likely increased. Our data suggest that there are changes in byssus production induced by hypoxia and acidification, which may affect predation rates on M. coruscus in the field. © 2015 Elsevier Ltd.


Hu M.,Shanghai Ocean University | Hu M.,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources | Hu M.,Zhejiang University | Wu F.,Shanghai Ocean University | And 6 more authors.
Journal of Hazardous Materials | Year: 2016

The single and combined effects of toxic cyanobacteria Microcystis aeruginosa and hypoxia on the energy budget of triangle sail mussel Hyriopsis cumingii were determined in terms of scope for growth (SfG). Mussels were exposed to different combinations of toxic M. aeruginosa (0%, 50%, and 100% of total dietary dry weight) and dissolved oxygen concentrations (1, 3, and 6.0 mg O2l-1) with a 3×3 factorial design for 14 days, followed by a recovery period with normal conditions for 7 days. Microcystin contents in mussel tissues increased with the increase in the exposed M. aeruginosa concentration at each sampling time. Adverse physiological responses of H. cumingii under toxic M. aeruginosa and hypoxic exposure were found in terms of clearance rate, absorption efficiency, respiration rate, excretion rate, and SfG. Results emphasized the importance of combined effects of hypoxia and toxic cyanobacteria on H. cumingii bioenergetic parameters, highlighted the interactive effects of toxic algae and hypoxia, and implied that the two stressors affected H. cumingii during the exposure period and showed carryover effects later. Thus, if H. cumingii is used as a bioremediation tool to eliminate M. aeruginosa, the waters should be oxygenated. © 2015 Elsevier B.V.


Wang S.-L.,Key Laboratory of Aquatic Genetic Resources and Their Utilization | Niu D.-H.,Key Laboratory of Aquatic Genetic Resources and Their Utilization | Jia M.-J.,Key Laboratory of Aquatic Genetic Resources and Their Utilization | Li J.-L.,Key Laboratory of Aquatic Genetic Resources and Their Utilization | And 2 more authors.
Conservation Genetics | Year: 2010

Nine polymorphic microsatellite loci were isolated and characterized from an AC-enriched genomic library of Bullacta exarata. They were polymorphic in 30 individuals from Qidong in Jiangsu Province, China. The number of alleles per polymorphic loci varied from 7 to 14 and the values of observed heterozygosity and expected heterozygosity ranged from 0. 2593 to 0. 9667 and from 0. 7090 to 0. 9164, respectively. Six of the nine microsatellites conformed to Hardy-Weinberg equilibrium, and inherited independently. These informative microsatellite markers will be useful in studies of population genetic structure for this species. © 2009 Springer Science+Business Media B.V.


Rupia E.J.,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources | Rupia E.J.,Shanghai Ocean University | Binning S.A.,University of Neuchatel | Roche D.G.,University of Neuchatel | And 2 more authors.
Journal of Animal Ecology | Year: 2016

Survival depends on appropriate behavioural and physiological responses to danger. In addition to active ‘fight-flight’ defence responses, a passive ‘freeze-hide’ response is adaptive in some contexts. However, the physiological mechanisms determining which individuals choose a given defence response remain poorly understood. We examined the relationships among personality, metabolic performance and physiological stress responses across an environmental gradient in the olive flounder, Paralichthys olivaceus. We employed four behavioural assays to document the existence of two distinct behavioural types (‘bold’ and ‘shy’) in this species. We found consistent metabolic differences between individuals of a given behavioural type across an environmental gradient: shy individuals had overall lower aerobic scope, maximum metabolic rate and standard metabolic rate than bold individuals in both high (25 ppt) and low (3 ppt) salinity. These behavioural and metabolic differences translated into divergent physiological responses during acute stress: shy individuals adopted a passive ‘freeze-hide’ response by reducing their oxygen consumption rates (akin to shallow breathing) whereas bold individuals adopted an active ‘fight-flight’ response by increasing their rates of respiration. These distinct defence strategies were repeatable within individuals between salinity treatments. Although it has been suggested theoretically, this is the first empirical evidence that the metabolic response to stressful situations differs between bold and shy individuals. Our results emphasize the importance of incorporating physiological measures to understand the mechanisms driving persistent inter-individual differences in animals. © 2016 The Authors. Journal of Animal Ecology © 2016 British Ecological Society


Bai Z.Y.,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources | Niu D.H.,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources | Le Li J.,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources | Le Li J.,Shanghai Ocean University
Conservation Genetics Resources | Year: 2011

Hyriopsis cumingii is the most important species for freshwater pearl culture in China. Seventeen polymorphic microsatellite repeat markers were identified from mantle expressed sequence tags of Hyriopsis cumingii. The number of alleles per locus ranged from two to 22, the observed and expected heterozygosities ranged from 0.042 to 1.000 and from 0.061 to 0.962, respectively. These markers should be useful for population genetics studies, parentage and genome mapping in this species. © Springer Science+Business Media B.V. 2011.


Wang Y.,Shanghai Ocean University | Wang Y.,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources | Li L.,Shanghai Ocean University | Li L.,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources | And 4 more authors.
Science of the Total Environment | Year: 2015

Anthropogenic CO2 emissions have caused seawater temperature elevation and ocean acidification. In view of both phenomena are occurring simultaneously, their combined effects on marine species must be experimentally evaluated. The purpose of this study was to estimate the combined effects of seawater acidification and temperature increase on the energy budget of the thick shell mussel Mytilus coruscus. Juvenile mussels were exposed to six combined treatments with three pH levels (8.1, 7.7 and 7.3)×two temperatures (25°C and 30°C) for 14d. We found that clearance rates (CRs), food absorption efficiencies (AEs), respiration rates (RRs), ammonium excretion rates (ER), scope for growth (SFG) and O:N ratios were significantly reduced by elevated temperature sometimes during the whole experiments. Low pH showed significant negative effects on RR and ER, and significantly increased O:N ratios, but showed almost no effects on CR, AE and SFG of M. coruscus. Nevertheless, their interactive effects were observed in RR, ER and O:N ratios. PCA revealed positive relationships among most physiological indicators, especially between SFG and CR under normal temperatures compared to high temperatures. PCA also showed that the high RR was closely correlated to an increasing ER with increasing pH levels. These results suggest that physiological energetics of juvenile M. coruscus are able to acclimate to CO2 acidification with a little physiological effect, but not increased temperatures. Therefore, the negative effects of a temperature increase could potentially impact the ecophysiological responses of M. coruscus and have significant ecological consequences, mainly in those habitats where this species is dominant in terms of abundance and biomass. © 2015 Elsevier B.V.


Tao X.-M.,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources | Wang X.-Y.,Urban Water Engineering | Wang L.-Q.,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources | Zheng X.-Y.,Shanghai Aquatic Environment Engineering Company Ltd | Zhang W.,Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources
Journal of Ecology and Rural Environment | Year: 2013

Community structure and species diversity of metazoan zooplankton was investigated monthly from May 2009 to March 2010 in Gehu Lake. A total of one hundred species of metazoan zooplankton were found and identified as 52 species of Rotifera under 25 genera, 21 species of Cladocera under 12 genera, and 27 species of Copepoda under 14 genera. The community was dominated mainly by Brachionus calyciflorus, Brachionus angularis, Polyarthra trigla, copepodid and Bosmina longirostris, with rotifera accounting for 98.7% of the total. The density of the zooplankton was 1 572 L-1 on average annually and peaked in spring, while the biomass of the zooplankton was 2.34 mg • L-1 on average annually and peaked in summer. The biomass of Cladocera was significantly and positively related to the density of Copepoda (r = 0.516, P < 0.01), whereas the biomass of Rotifera negatively related to the biomass of Copepoda. Density and biomass of the metazoan zooplankton varied sharply with the season, showing an descending order of spring, summer, autumn, and winter, and of summer, spring, autumn, and winter, respectively. The values of the Shannon-Wiener diversity index, Margalef species richness index and Pielou index indicate that the Gehu Lake was light to moderate in pollution.

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