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Ren Z.H.,Sichuan Agricultural University | Ren Z.H.,Sichuan Province Key Laboratory of Animal Disease & Human Health | Ren Z.H.,Key Laboratory of Environmental Hazard and Human Health of Sichuan Province | Zhou R.,Sichuan Agricultural University | And 27 more authors.
Food and Agricultural Immunology | Year: 2014

The aim of this study was to evaluate the effects of the Fusarium toxin zearalenone (ZEA) and/or deoxynivalenol (DON) on the serum IgA, IgG and IgM levels in mice. In our study, 360 healthy adult female mice were randomly assigned to nine groups for a 12-day study. Mice of all groups were given a 4-day continuous intraperitoneal injection with different concentrations of ZEA alone, DON alone and the mixture of ZEA and DON, individually. Blood samples were collected on days 0, 3, 5, 8 and 12 of the experiment. The experiment results showed that all the experimental groups could cause the dysregulation of the immunoglobulin, thus affecting the humoral immune of mice. The mixture groups, especially in the group with higher concentrations of ZEA and DON (Group D2Z2), showed more obvious effect on the dysregulation of the immunoglobulin. © 2013, © 2013 Taylor & Francis. Source


Ren Z.H.,Sichuan Agricultural University | Ren Z.H.,Sichuan Province Key Laboratory of Animal Disease and Human Health | Ren Z.H.,Key Laboratory of Environmental Hazard and Human Health of Sichuan Province | Deng H.D.,Sichuan Agricultural University | And 33 more authors.
Environmental Toxicology and Pharmacology | Year: 2016

This study aimed to evaluate the effects of the Fusarium toxin zearalenone (ZEA) and deoxynivalenol (DON) on splenic antioxidant functions, IFN levels, and T-cell subsets in mice. Herein, 360 mice were assigned to nine groups for a 12-day study. Mice were administered an intraperitoneal injection for 4 consecutive days with different concentrations of ZEA alone, DON alone, or ZEA. +. DON. Spleen and blood samples were collected on days 0, 3, 5, 8, and 12. Mice in each of the experimental groups showed dysreglated splenic antioxidant functions, IFN levels, and T-cell subset frequencies, suggesting that the immune system had been affected. The ZEA. +. DON-treated groups, especially the group that received a higher concentration of ZEA. +. DON (Group D2Z2), showed more obvious effects on the dysregulation of splenic antioxidant functions, IFN levels, and T-cell subsets. This finding suggested that DON and ZEA exerted synergistic effects. © 2015 Elsevier B.V. Source


Liang Z.,Sichuan Agricultural University | Liang Z.,Sichuan Province Key Laboratory of Animal Disease and Human Health | Liang Z.,Key Laboratory of Environmental Hazard and Human Health of Sichuan Province | Ren Z.,Sichuan Agricultural University | And 26 more authors.
Environmental Toxicology and Pharmacology | Year: 2015

This study was performed to investigate the individual and combined toxic effects of deoxynivalenol (DON) and zearalenone (ZEA) on mouse kidney. A total of 360 female mice were divided into nine groups. Each group received intraperitoneal injection of solvent (control), DON, ZEA, or DON + ZEA four times for 12. d. Results showed that ZEA and/or DON increased the apoptosis rate in the kidney, as well as the levels of serum creatinine and blood urea nitrogen. DON and/or ZEA also induced renal oxidative stress as indicated by increased malondialdehyde concentration and nitric oxide level and reduced superoxide dismutase enzyme activity and hydroxyl radical inhibiting capacity. The observed changes were dose and time dependent. This study reports that DON and/or ZEA induced apoptosis, dysfunction, and oxidative stress in mouse kidney. Furthermore, the combination of DON + ZEA exhibited a sub-additive nephrotoxic effect. © 2015. Source


Ren Z.,Sichuan Agricultural University | Ren Z.,Sichuan Province Key Laboratory of Animal Disease and Human Health | Ren Z.,Key Laboratory of Environmental Hazard and Human Health of Sichuan Province | Wang Y.,Sichuan Agricultural University | And 24 more authors.
Environmental Toxicology and Pharmacology | Year: 2015

We investigated the immunotoxicity and cytotoxicity of deoxynivalenol (DON), a mycotoxin, and the mechanism by which it induces apoptosis. Chicken splenic lymphocytes treated with 0-50. μg/mL DON for 48. h inhibited growth of splenic lymphocytes in a dose-dependent manner, as revealed by the Cell Counting Kit-8 (CCK-8) bioassay. Annexin V-fluorescein isothiocyanate staining indicated that the number of apoptotic and necrotic cells were significantly higher compared with the control (P<. 0.01). DON treatment induced ROS accumulation, resulting in reduced mitochondrial transmembrane potential, as detected by flow cytometry and 2',7'-dichlorofluorescein acetate and rhodamine 123 labeling, respectively. Enzyme linked immunosorbent assays revealed that the concentrations of p53, Bax, Bak-1, and Caspase-3 increased with increasing DON concentration (P<. 0.05 or P<. 0.01), whereas the concentrations of Bcl-2 decreased (P<. 0.01) compared with the control. These data suggest that DON induces apoptosis in splenic lymphocytes via a ROS-mediated mitochondrial pathway. © 2014 Published by Elsevier B.V. Source

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