Key Laboratory of Environment Correlative Dietology

Key Laboratory of Environment Correlative Dietology

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Hou H.-J.,Huazhong Agricultural University | Zhang X.-H.,Huazhong Agricultural University | Huang D.-K.,Huazhong Agricultural University | Ding X.,Huazhong Agricultural University | And 7 more authors.
Applied Catalysis B: Environmental | Year: 2017

A π-conjugated microporous poly(benzothiadiazole) (hereafter denoted as BBT) is synthesized through palladium-catalyzed Sonogashira-Hagihara cross-coupling polycondensation, and used as a novel organic semiconductor photocatalyst for both photocatalytic H2 production and pollutant degradation under visible light (λ > 420 nm) irradiation. Furthermore, BBT/TiO2 heterojunction is conveniently fabricated through an in-situ polycondensation procedure of 4,7-dibromobenzo[c][1,2,5]thiadiazole and 1,3,5-triethynylbenzene in the presence of commercial TiO2. After optimizing the composition ratio, the resultant BBT/TiO2 heterojunction exhibited dramatically enhanced visible-light-responsive photocatalytic activities (∼18.0 and 20.4 times higher activity for H2 evolution and ciprofloxacin degradation, respectively) as compared BBT alone. Detailed investigations revealed that the BBT/TiO2 heterojunction interface can accelerate the photogenerated electron transferring from BBT to TiO2, and then improve the photoactivity. The present work exhibits some interesting points and dramatic improvement of photoactivity when an organic semiconductor is combined with an inorganic one, which provides a novel direction to exploit and fabricate photocatalyst for solar energy conversion and pollutant degradation. © 2016 Elsevier B.V. All rights reserved.


Fan C.,Key Laboratory of Environment Correlative Dietology | Tian M.,Key Laboratory of Environment Correlative Dietology | Yang Z.,Key Laboratory of Environment Correlative Dietology | Liu F.,Key Laboratory of Environment Correlative Dietology | Pan S.,Key Laboratory of Environment Correlative Dietology
Journal of Food Processing and Preservation | Year: 2017

Dietary fiber plays an important role in the prevention and reduction of chronic diseases. This study evaluated the effect of drying methods (hot air drying, vacuum drying, and freeze-drying) on physicochemical properties and in vitro hypoglycemic effects of soluble and insoluble dietary fiber from orange peels. Freeze-drying significantly decreased the bulk density of the soluble and insoluble dietary fiber and increased the viscosity of soluble dietary fiber solutions (36.33 mPa s). Hot air drying caused a decrease in the molecular weight of soluble dietary fiber compared with freeze-drying. Freeze-dried dietary fibers exhibited a higher glucose adsorption capacity and glucose dialysis retardation index than fibers from other drying conditions, and this method enhanced the inhibitory effect of dietary fiber on α-amylase activity. Based on these results, freeze-drying is suggested for use in dietary fiber processing to obtain high-quality products for use in functional foods. Practical applications: When formulating fiber-rich food products, the functional properties of dietary fiber must be considered. The functional properties depend on the processing conditions and physicochemical properties of the fiber. The results show that freeze-drying has significant advantages in improving physicochemical properties and in vitro hypoglycemic effects of dietary fiber, and the application of freeze-drying in dietary fiber processing could enhance its nutrient and commercial values. © 2017 Wiley Periodicals, Inc.


Zhang X.-H.,Huazhong Agricultural University | Wang X.-P.,Huazhong Agricultural University | Xiao J.,Huazhong Agricultural University | Wang S.-Y.,Huazhong Agricultural University | And 7 more authors.
Journal of Catalysis | Year: 2017

Some conjugated polymer organic semiconductors with linear or network structures and tunable band gaps are found to be efficient metal-free photocatalysts for H2 production. In the present work, three 1,4-diethynylbenzene-based linear conjugated polymer organic semiconductors (named P7-E, B-BT-1,4-E, and P17-E) were synthesized by palladium-catalyzed Sonogashira–Hagihara cross-coupling polycondensation. Compared with three reported 1,4-benzene-based linear conjugated polymer organic semiconductors (P7, B-BT-1,4, and P17), the ethynyl group is introduced into their backbones and the absorption edge is extended by 150–190 nm to the red/NIR light region (up to ∼750 nm). Importantly, a negative shift of LUMO levels and enhanced photocurrent intensity are also observed, and their visible-light-induced H2 production activity is improved dramatically. P7-E exhibits the highest H2 production rate of 180.7 μmol/h under λ > 420 nm irradiation even without Pt co-catalyst loaded. Moreover, the apparent quantum yield (AQY) value of P7-E is 4.2% at 420 nm, which is much higher than that reported so far for P7. The present result indicates that small changes in the chemical structure of conjugated polymers can significantly tune their optical and photocatalytic properties, which provides a new direction for attaining more efficient organic conjugated polymer photocatalysts. © 2017 Elsevier Inc.


Shao Y.,Key Laboratory of Environment Correlative Dietology | Yang S.,Huazhong Agricultural University | Zhang Z.,Hubei Academy of Agricultural science | Zhou Y.,China Institute of Technology | Chen F.,Key Laboratory of Environment Correlative Dietology
Mycologia | Year: 2016

Skn7, a response regulator (RR), is associated with oxidative stress adaptation, hypo-osmotic stress response, fungicide sensitivity, cell wall biosynthesis, cell cycle regulation, sexual mating, and sporulation in many filamentous fungi and yeasts. In this study a Skn7-like protein gene mrskn7 (Monascus ruber skn7) was isolated, sequenced, and disrupted to investigate its function in M. ruber. Bioinformatics predicted that the deduced protein encoded by mrskn7 contained the conserved DNA-binding and signal-receiver domains similar to the Skn7-like protein structure in other filamentous fungi. The Δmrskn7 strain produced fewer conidia and less mycotoxin, demonstrated increased sensitivity to peroxide but the same level of osmotic resistance to NaCl and glycerol with the wildtype. Additionally, cleistothecia observed at different time point showed a different morphology between the wild-type and the Δmrskn7 strain, suggesting the involvement of mrskn7 in sexual development of M. ruber. These results indicated that mrskn7 plays important roles in asexual and sexual development, the production of mycotoxin as well as regulation of oxidative stress signal in M. ruber. © 2016 by The Mycological Society of America, Lawrence, KS 66044-8897.


Liu Q.,Key Laboratory of Environment Correlative Dietology | Liu Q.,China Agricultural University | Liu Q.,Hubei University | Si T.,Key Laboratory of Environment Correlative Dietology | And 9 more authors.
Reproductive Health | Year: 2015

Background: The decreased reproductive capacity of men is an important factor contributing to infertility. Accumulating evidence has shown that Electromagnetic radiation potentially has negative effects on human health. However, whether radio frequency electromagnetic radiation (RF-EMR) affects the human reproductive system still requires further investigation. Therefore, The present study investigates whether RF-EMR at a frequency of 900 MHz can trigger sperm cell apoptosis and affect semen morphology, concentration, and microstructure. Methods: Twenty four rats were exposed to 900 MHz electromagnetic radiation with a special absorption rate of 0.66 ± 0.01 W/kg for 2 h/d. After 50d, the sperm count, morphology, apoptosis, reactive oxygen species (ROS), and total antioxidant capacity (TAC), representing the sum of enzymatic and nonenzymatic antioxidants, were investigated. Western blotting and reverse transcriptase PCR were used to determine the expression levels of apoptosis-related proteins and genes, including bcl-2, bax, cytochrome c, and capase-3. Results: In the present study, the percentage of apoptotic sperm cells in the exposure group was significantly increased by 91.42 % compared with the control group. Moreover, the ROS concentration in exposure group was increased by 46.21 %, while the TAC was decreased by 28.01 %. Radiation also dramatically decreased the protein and mRNA expression of bcl-2 and increased that of bax, cytochrome c, and capase-3. Conclusion: RF-EMR increases the ROS level and decreases TAC in rat sperm. Excessive oxidative stress alters the expression levels of apoptosis-related genes and triggers sperm apoptosis through bcl-2, bax, cytochrome c and caspase-3 signaling pathways. © 2015 Liu et al.


Peng B.,Key Laboratory of Environment Correlative Dietology | Lei Y.,Northwest University, China | Zhao H.,Northwest University, China | Cui L.,Northwest University, China
Journal of Food Science and Technology | Year: 2015

Apple wine is a fermented alcoholic beverage made from apple juice. To ensure optimal apple wine processing performance and premium final product quality, an optimized selection of various parameters for apple wine fermentation process is imperative. This study is aimed at optimizing the fermentation process based on three primary fermentation parameters, including fermentation temperature, initial pH value and inoculum volume, to improve apple wine quality using response surface methodology (RSM) coupled with central composite rotatable design method (CCRD). Based on the experimental data analysis a second order response surface model was developed to describe the relationship between fermentation temperature, initial pH value, and inoculum volume with respect to the sensory score of apple wine. The results of analysis of variance (ANOVA) showed that the model established by response surface quadratic regression was adequate for predicting apple wine quality. It was observed that the quality of apple wine was significantly influenced by fermentation temperature (p ≤ 0.01), followed by initial pH value (p ≤ 0.01) and inoculum volume (p ≤ 0.01). The temperature of 20 °C, initial pH value of 3.6 and inoculum volume of 9 % were found to be the optimal fermentation condition for ensuring premium quality of apple wine. © 2015 Association of Food Scientists & Technologists (India)


Khan S.A.,Huazhong Agricultural University | Liu X.,Huazhong Agricultural University | Liu X.,Key Laboratory of Environment Correlative Dietology | Shah B.R.,Huazhong Agricultural University
Journal of Animal and Plant Sciences | Year: 2014

This study evaluated the acute toxicity effects of Pb2+ on essential trace metals behavior and histopathology of Crucian carp (Carassius auratus gibelio). The median lethal concentration (96 h LC50) of Pb2+ was determined to be 29.07 mgL−1 in a semi-static bioassay. The fish were exposed to sublethal concentration of 5mgL−1 as environmentally relevant Pb2+ for a period of 96 h. Trace metals (Pb, Cu, Zn, Fe and Ca) levels were determined in the gill, liver, kidney and muscles tissue by ICP-OES. Regression analysis revealed that Pb2+ exposure had negative impact on the level of Cu, Zn, Fe and Ca in certain tissues (P<0.05;P<0.01). Histopathological changes in the gills of exposed fish were characterized by lamellar shrinkage, disruption of cartilaginous core, epithelial lifting, lamellar shortening with desquamation and curling of the secondary lamella. The trunk kidney had severe shrinkage of glomeruli, hypoplasia of hemopoietic tissue as well as mild glomerular and tubular necrosis. The liver showed cellular edema, necrosis of hepatocytes with nuclear degeneration and pyknotic nuclei. The brain exhibited severe proliferation of glial cells, cellular necrosis, severe perivascular edema and satellitosis. These results clearly depict the deleterious effects of Pb2+ on trace metal metabolism and tissue architecture of Crucain carp. © 2014, Pakistan Agricultural Scientists Forum. All rights reserved.


Chen T.,Huazhong Agricultural University | Chen Y.-H.,Huazhong Agricultural University | Chen F.-S.,Huazhong Agricultural University | Chen F.-S.,Key Laboratory of Environment Correlative Dietology
Modern Food Science and Technology | Year: 2013

The effects of soup obtained from commercial grey sufu on the main compositions, texture and odour were researched. The results showed that compared to the control of sufu (no addition of soup), the contents of protein and crude fat were lower in the sample sufu (adding of soup), but the free amino acid content was higher in sample sufu during ripening process. After ripening for 49 d, the free amino acid content in sample sufu reached to 11.70% (dry weight), which was 60% higher than that achieved in control. The hardness and resilience were lower in sample sufu body, but the cohesiveness was higher. After ripening for 49 d, the values of hardness, cohesiveness and resilience were 213.75 g, 0.37 and 0.35 in sample sufu body and 258.28 g, 0.29 and 0.43 in the control, respectively. The textural parameters (hardness, cohesiveness and resilience) showed a significant correlation with protein content in the two kinds of sufu. It produced volatile sulfide and indole after adding the soup. But when the soup centrifuged by high speed or filtered by microfiltration membrane, it could not produce these substances.


Chen M.,Huazhong Agricultural University | Chen M.,Key Laboratory of Environment Correlative Dietology | Yin T.,Huazhong Agricultural University | Yin T.,Key Laboratory of Environment Correlative Dietology | And 5 more authors.
Starch/Staerke | Year: 2014

Octenyl succinic anhydride (OSA) modified starch from waxy rice was prepared using dry media milling. The characteristics of OSA were investigated by laser particle analyzer, FT-IR spectroscopy, XRD, and DSC. Degree of substitution (DS) and reaction efficiency (RE) were significantly (p<0.05) influenced by sodium hydroxide concentration, OSA concentration, and mechanical activation time. The suitable processing conditions for preparing octenyl succinic anhydride starch (OSA starch) were determined to be 0.9 g sodium hydroxide per 100 g starch, 4 g OSA per 100 g starch, and 20 h of mechanical activation. Intensities of characteristic peaks at 1724 and 1572 cm-1 in FT-IR spectrogram increased with DS, which confirmed the successful introduction of ester carbonyl groups into the starch. Size distribution analysis and XRD indicated that the increased DS and RE by dry media milling were attributed to both the decrease of starch granule size and the destruction of the crystalline structure. DSC results showed that the gelatinization temperature of the OSA starch decreased with the increase of DS. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


PubMed | Key Laboratory of Environment Correlative Dietology
Type: | Journal: Reproductive health | Year: 2015

The decreased reproductive capacity of men is an important factor contributing to infertility. Accumulating evidence has shown that Electromagnetic radiation potentially has negative effects on human health. However, whether radio frequency electromagnetic radiation (RF-EMR) affects the human reproductive system still requires further investigation. Therefore, The present study investigates whether RF-EMR at a frequency of 900 MHz can trigger sperm cell apoptosis and affect semen morphology, concentration, and microstructure.Twenty four rats were exposed to 900 MHz electromagnetic radiation with a special absorption rate of 0.660.01 W/kg for 2 h/d. After 50d, the sperm count, morphology, apoptosis, reactive oxygen species (ROS), and total antioxidant capacity (TAC), representing the sum of enzymatic and nonenzymatic antioxidants, were investigated. Western blotting and reverse transcriptase PCR were used to determine the expression levels of apoptosis-related proteins and genes, including bcl-2, bax, cytochrome c, and capase-3.In the present study, the percentage of apoptotic sperm cells in the exposure group was significantly increased by 91.42% compared with the control group. Moreover, the ROS concentration in exposure group was increased by 46.21%, while the TAC was decreased by 28.01%. Radiation also dramatically decreased the protein and mRNA expression of bcl-2 and increased that of bax, cytochrome c, and capase-3.RF-EMR increases the ROS level and decreases TAC in rat sperm. Excessive oxidative stress alters the expression levels of apoptosis-related genes and triggers sperm apoptosis through bcl-2, bax, cytochrome c and caspase-3 signaling pathways.

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