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Liu Q.,Key Laboratory of Environment Correlative Dietology | Liu Q.,China Agricultural University | Liu Q.,Hubei University | Si T.,Key Laboratory of Environment Correlative Dietology | And 9 more authors.
Reproductive Health | Year: 2015

Background: The decreased reproductive capacity of men is an important factor contributing to infertility. Accumulating evidence has shown that Electromagnetic radiation potentially has negative effects on human health. However, whether radio frequency electromagnetic radiation (RF-EMR) affects the human reproductive system still requires further investigation. Therefore, The present study investigates whether RF-EMR at a frequency of 900 MHz can trigger sperm cell apoptosis and affect semen morphology, concentration, and microstructure. Methods: Twenty four rats were exposed to 900 MHz electromagnetic radiation with a special absorption rate of 0.66 ± 0.01 W/kg for 2 h/d. After 50d, the sperm count, morphology, apoptosis, reactive oxygen species (ROS), and total antioxidant capacity (TAC), representing the sum of enzymatic and nonenzymatic antioxidants, were investigated. Western blotting and reverse transcriptase PCR were used to determine the expression levels of apoptosis-related proteins and genes, including bcl-2, bax, cytochrome c, and capase-3. Results: In the present study, the percentage of apoptotic sperm cells in the exposure group was significantly increased by 91.42 % compared with the control group. Moreover, the ROS concentration in exposure group was increased by 46.21 %, while the TAC was decreased by 28.01 %. Radiation also dramatically decreased the protein and mRNA expression of bcl-2 and increased that of bax, cytochrome c, and capase-3. Conclusion: RF-EMR increases the ROS level and decreases TAC in rat sperm. Excessive oxidative stress alters the expression levels of apoptosis-related genes and triggers sperm apoptosis through bcl-2, bax, cytochrome c and caspase-3 signaling pathways. © 2015 Liu et al. Source


Zhang L.,Huazhong Agricultural University | Liu X.,Huazhong Agricultural University | Liu X.,Key Laboratory of Environment Correlative Dietology | Qiu J.,Huazhong Agricultural University | And 3 more authors.
International Journal of Polymer Analysis and Characterization | Year: 2014

A molecularly imprinted polymer was prepared using diethylphosphonoacetic acid (DPA) as a dummy template for use as an artificial receptor for organophosphrous pesticides (OPPs). Scanning electron microscopy (SEM) was employed to observe the morphology of the prepared polymer. Ultraviolet spectroscopy and 1H NMR analysis reveal that DPA and the monomer integrate by hydrogen bonding with a molar ratio of 1:2. A static adsorption experiment, Scatchard analysis, and dynamic adsorption and selective binding tests demonstrated that the obtained polymer can effectively bind a group of OPPs containing dimethoxyl or diethyoxyl. © 2014 Taylor & Francis Group, LLC. Source


Khan S.A.,Huazhong Agricultural University | Liu X.,Huazhong Agricultural University | Liu X.,Key Laboratory of Environment Correlative Dietology | Shah B.R.,Huazhong Agricultural University
Journal of Animal and Plant Sciences | Year: 2014

This study evaluated the acute toxicity effects of Pb2+ on essential trace metals behavior and histopathology of Crucian carp (Carassius auratus gibelio). The median lethal concentration (96 h LC50) of Pb2+ was determined to be 29.07 mgL−1 in a semi-static bioassay. The fish were exposed to sublethal concentration of 5mgL−1 as environmentally relevant Pb2+ for a period of 96 h. Trace metals (Pb, Cu, Zn, Fe and Ca) levels were determined in the gill, liver, kidney and muscles tissue by ICP-OES. Regression analysis revealed that Pb2+ exposure had negative impact on the level of Cu, Zn, Fe and Ca in certain tissues (P<0.05;P<0.01). Histopathological changes in the gills of exposed fish were characterized by lamellar shrinkage, disruption of cartilaginous core, epithelial lifting, lamellar shortening with desquamation and curling of the secondary lamella. The trunk kidney had severe shrinkage of glomeruli, hypoplasia of hemopoietic tissue as well as mild glomerular and tubular necrosis. The liver showed cellular edema, necrosis of hepatocytes with nuclear degeneration and pyknotic nuclei. The brain exhibited severe proliferation of glial cells, cellular necrosis, severe perivascular edema and satellitosis. These results clearly depict the deleterious effects of Pb2+ on trace metal metabolism and tissue architecture of Crucain carp. © 2014, Pakistan Agricultural Scientists Forum. All rights reserved. Source


Khan S.A.,Huazhong Agricultural University | Zhou P.,Huazhong Agricultural University | Liu X.,Huazhong Agricultural University | Liu X.,Key Laboratory of Environment Correlative Dietology | And 4 more authors.
Acta Biochimica Polonica | Year: 2015

Toxicity of Pb2+ and Cd2+ is a widespread issue in the world; however, few studies have been conducted to understand their effect at environmentally realistic concentration in a mixture. In the present study, Crucian carp was exposed to Pb2+ (30 μg · l-1), Cd2+ (100 μg · l-1) and their mixture (30+100 μg · l-1) for 96 h and 21 d period to assess changes in the liver and muscle vitamin A and E content, and hematological and serum biochemical parameters. The results indicated significant decline in the level of antioxidant vitamins A, E and alterations in the hematological and serum biochemical indices. The toxicity revealed anemia, impairment of the liver and kidney with evident responses after 21 d exposure due to additive effect of Pb2+ and Cd2+ in mixture. Moreover, the differential response of vitamins A, E and blood parameters to low levels of waterborne Pb2+ and Cd2+ in freshwater fish can be used as biomarkers for monitoring contamination of aquatic environment. Source


Peng B.,Key Laboratory of Environment Correlative Dietology | Lei Y.,Northwest University, China | Zhao H.,Northwest University, China | Cui L.,Northwest University, China
Journal of Food Science and Technology | Year: 2015

Apple wine is a fermented alcoholic beverage made from apple juice. To ensure optimal apple wine processing performance and premium final product quality, an optimized selection of various parameters for apple wine fermentation process is imperative. This study is aimed at optimizing the fermentation process based on three primary fermentation parameters, including fermentation temperature, initial pH value and inoculum volume, to improve apple wine quality using response surface methodology (RSM) coupled with central composite rotatable design method (CCRD). Based on the experimental data analysis a second order response surface model was developed to describe the relationship between fermentation temperature, initial pH value, and inoculum volume with respect to the sensory score of apple wine. The results of analysis of variance (ANOVA) showed that the model established by response surface quadratic regression was adequate for predicting apple wine quality. It was observed that the quality of apple wine was significantly influenced by fermentation temperature (p ≤ 0.01), followed by initial pH value (p ≤ 0.01) and inoculum volume (p ≤ 0.01). The temperature of 20 °C, initial pH value of 3.6 and inoculum volume of 9 % were found to be the optimal fermentation condition for ensuring premium quality of apple wine. © 2015 Association of Food Scientists & Technologists (India) Source

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