Key Laboratory of Energy Microbiology and Application

Chengdu, China

Key Laboratory of Energy Microbiology and Application

Chengdu, China
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Ma S.,Biogas Institute of Ministry of Agriculture | Ma S.,Key Laboratory of Energy Microbiology and Application | Huang Y.,Biogas Institute of Ministry of Agriculture | Huang Y.,Key Laboratory of Energy Microbiology and Application | And 12 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2017

A thermophilic, anaerobic, fermentative bacterium, strain A6T, was obtained from an anaerobic batch digester treating animal manure and rice straw. Cells were Gram-stain-positive, slightly curved rods with a size of 0.6–1×2.5–8.2 μm, nonmotile and produced terminal spores. The temperature, pH and NaCl concentration ranges for growth were 40–60 °C, 6.5–8.0 and 0–15.0 g l–1, with optimum growth noted at 50–55 °C, pH 7.5 and in the absence of NaCl, respectively. Yeast extract was required for growth. D-Glucose, maltose, D-xylose, D-galactose, D-fructose, D-ribose, lactose, raffinose, sucrose, D-arabinose, cellobiose, D-mannose and yeast extract were used as carbon and energy sources. The fermentation products from glucose were ethanol, lactate, acetate, propionate, butyrate, valerate, iso-butyrate, iso-valerate, H2 and CO2. The G+C content of the genomic DNA was 36.6 mol%. The predominant fatty acids were C16: 0, iso-C17: 1, C14: 0, C16: 1ω7c, C16: 0 N-alcohol and C13: 0 3- OH. Respiratory quinones were not detected. The polar lipid profile comprised phosphoglycolipids, phospholipids, glycolipids, a diphosphatidylglycerol, a phosphatidylglycerol and an unidentified lipid. Phylogenetic analyses of the 16S rRNA gene sequence indicated that the strain was closely related to Defluviitalea saccharophila DSM 22681T with a similarity of 96.0%. Based on the morphological, physiological and taxonomic characterization, strain A6T is considered to represent a novel species of the genus Defluviitalea, for which the name Defluviitalea raffinosedens sp. nov. is proposed. The type strain is A6T (=DSM 28090T=ACCC 19951T). © 2017 IUMS.


Tan F.,China Institute of Technology | Wu B.,China Institute of Technology | Dai L.,China Institute of Technology | Zhu Q.,China Institute of Technology | And 8 more authors.
Chinese Journal of Applied and Environmental Biology | Year: 2014

Cellulosic herbaceous energy plant, which has high biomass yield, cellulose and hemicellulose content, is one of the most promising biomass resources positively influencing the environment. It has been used as a preferred biomass energy plant in Europe and the United States. This paper introduces the characteristics of high yield, well-adapted and wide application of some main herbaceous energy plants, such as switchgrass, miscanthus and bamboo reed. It also summarizes the research status of cellulosic herbaceous energy plant and application of genetic engineering technology on decreasing lignin content and overexpression cellulase in plants. This article further presents the problems, such as resource evaluation, lagging of breeding and imperfect technology, and prospects of developing celluslosic herbaceous energy plant in China. If the abundant resource of herbaceous energy plant of our country could be efficiently utilized, it can not only alleviate nergy crisis, but also make full use of the barren land, change planting structure, increase farmers' income, and in the long run promote the national economy.


Liu H.,Key Laboratory of Energy Microbiology and Application | Lan G.,Sichuan University | Liu Q.,Key Laboratory of Energy Microbiology and Application | Zhang W.,Key Laboratory of Energy Microbiology and Application | And 3 more authors.
Shengwu Gongcheng Xuebao/Chinese Journal of Biotechnology | Year: 2010

To explore new microbial resources in deep subsurface oil reservoirs, strain DL-7 was isolated with Hungate technology from oil reservoir water sampled from Dagang oilfield, China. Physiological and biochemical examinations showed that H2/CO2 is the unique substrate of the strain, which cannot metabolize formate, methanol, trimethylamine, acetate and other secondary alcohols. The optimum growth conditions were further identified to be 60°C, pH 7.0-7.5 and 0.25% NaCl. Moreover, the strain cannot grow without yeast extract. Analysis of its 16S rRNA sequence indicated that a similarity of 99.7% presents between the strain and the model species M. marburgensis DSM2133T (X15364). © 2010 CJB, All rights reserved.


He M.,Chinese Academy of Sciences | He M.,Key Laboratory of Energy Microbiology and Application | Hu Q.,Chinese Academy of Sciences | Hu Q.,Key Laboratory of Energy Microbiology and Application | And 6 more authors.
African Journal of Biotechnology | Year: 2010

Microbial oil is a promising new biodiesel resource, which have great potential in industrial-scale production. In our preliminary study, 57 oleaginous yeast with xylose assimilating capacity were isolated from 13 soil samples, 16 strains were identified as potential lipid biomass producer. Four strains which showed higher lipid content were used for further ethanol fermentation at different conditions. Strain 9-44 belonging to Pichia guillermondii showed the highest ethanol production (21.91 g/l), and the theoretical ethanol yield was 85.90%. Our study will be of great significance for coupling of lipid and bio-ethanol production, and also provide a choice of cellulocis biomass utilization. © 2010 Academic Journals.


Qiu T.,Beijing Academy of Agriculture and Forestry Sciences | Sun X.,Beijing Academy of Agriculture and Forestry Sciences | Wang X.,Beijing Academy of Agriculture and Forestry Sciences | Han M.,Beijing Academy of Agriculture and Forestry Sciences | And 4 more authors.
Advanced Materials Research | Year: 2011

Biogas fermentation is always limited or affected at low temperature conditions, one of key factors may be physiological adaption of methanogenic communities to low temperature. Howerer, biogas process could be stimulated and enhanced with addition of enriched consortia. Here, six psychroactive methanogenic consortiums were enriched as additives, and consortium E-1 was found to be most effective at 150C. The total biogas production addition with E-1 improved 39.3 % and 17.0% from pig manure and cow manure, respectively, when decreasing from 210C to 150C, which is a potential microbial addition for biogas fermentation at low temperature. © (2011) Trans Tech Publications.


Yang L.,Biogas Institute of Ministry of Agricultural | Yang L.,Key Laboratory of Energy Microbiology and Application | Ma S.,Biogas Institute of Ministry of Agricultural | Ma S.,Key Laboratory of Energy Microbiology and Application | And 4 more authors.
Chinese Journal of Applied and Environmental Biology | Year: 2011

An amphipsychrophilic methanogen 8-1 with growth temperature of 7∼40 °C, was isolated from the soil in the Ailao Mountains in Yunnan, China. The strain was gram-positive, non-motile, single, paired, or aggregated in growth, and the cell was irregularly crooked rod with diameter of 0.2-0.3 urn and length of 2∼5 μm. This strain could use hydrogen and carbon dioxide, or formate as substrates for its growth and methanogenesis, but it could not use methanol, trimethylamine, acetic acid and diatomic alcohol. Its optimal growth conditions: 35 °C, pH 7.5-8.5 and 0 mol/L NaCl, with the lowest growth temperature of 7 °C. Sequence analysis of the 16S rRNA genes of strain 8-1 and Methanobacterium subterraneum strain A8p (= DSM 11074T) revealed the similarities of them are 99%.


He M.,Chinese Academy of Agricultural Sciences | He M.,Key Laboratory of Energy Microbiology and Application | Hu Q.,Chinese Academy of Agricultural Sciences | Hu Q.,Key Laboratory of Energy Microbiology and Application | And 5 more authors.
Chinese Journal of Applied and Environmental Biology | Year: 2011

Different constructed wetland plants were systematically assessed for energy utilization by measuring their cellulosic contents, caloricvalues and hydrolyzate of NaOH-cellulase pretreatment. The results showed that the cellulose, hemicellulose and lignin contents of the fifteen different constructed wetland plants were 19.78% to 36.9%, 4.51% to 19.67% and 10.79% to 20.47%, respectively. All wetland plants used in this study showed the same thermal value (ranged from 14.002 to 17.839 MJ/kg) as corn stover. Among the five types of sugar existed in the hydrolyzate, glucose and xylose were the main components. The results indicated that the constructed wetland plant could be used as one of suitable raw material resources for biomass briquette fuel, biogas and bioethanol production, and the coupled mode of constructed wetland plant biomass and bioenergy also could be constructed in the future. Fig 2, Tab 2, Ref 22.


Feng Y.,Key Laboratory of Energy Microbiology and Application | Feng Y.,Biogas Institute | Cheng L.,Key Laboratory of Energy Microbiology and Application | Cheng L.,Biogas Institute | And 7 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2010

A novel thermophilic, strictly anaerobic, heterotrophic bacterium, strain 2SM-2T, was isolated from the Shengli oilfield, China. This organism was identified as a member of the order Thermotogales on the basis of its 16S rRNA gene sequence and the presence of an external membranous togalike structure. Cells stained Gram-negative, were non-motile, appeared as irregular cocci 0.7-0.9 mm in diameter, and occurred in clusters of two to six cells, with cells located within a ballooning toga-like membrane. Its optimum temperature, pH and NaCl concentration for growth were 65 °C, 7.0 and 15 g l1, respectively. Under the optimum growth conditions, the doubling time was approximately 105 min. Strain 2SM-2T fermented a variety of simple and complex substrates such as glucose, acetate, methanol, starch and peptone while reducing elemental sulfur, sulfate and thiosulfate. The end products identified during growth on glucose were acetate, lactate, L-alanine, H 2 and CO2. The DNA G+C content of this organism was 36.4 mol%. The results of 16S rRNA gene-based sequence comparisons revealed that the strain represented a new lineage within the family Thermotogaceae of the order Thermotogales. Based on the phenotypic and phylogenetic characteristics, it is proposed that this organism represents a novel species in a new genus within the family Thermotogaceae, for which the name Thermococcoides shengliensis gen. nov., sp. nov. is proposed. The type strain is 2SM-2T (=ACCC 00496T=DSM 22460T). © 2010 IUMS.


PubMed | Key Laboratory of Energy Microbiology and Application
Type: Journal Article | Journal: Sheng wu gong cheng xue bao = Chinese journal of biotechnology | Year: 2010

To explore new microbial resources in deep subsurface oil reservoirs, strain DL-7 was isolated with Hungate technology from oil reservoir water sampled from Dagang oilfield, China. Physiological and biochemical examinations showed that H2/CO2 is the unique substrate of the strain, which cannot metabolize formate, methanol, trimethylamine, acetate and other secondary alcohols. The optimum growth conditions were further identified to be 60 degrees C, pH 7.0-7.5 and 0.25% NaCl. Moreover, the strain cannot grow without yeast extract. Analysis of its 16S rRNA sequence indicated that a similarity of 99.7% presents between the strain and the model species M. marburgensis DSM2133T (X15364).

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