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Jiang S.-L.,Fuyang Teachers College | Jiang S.-L.,Key Laboratory of Embryo Development and Reproductive Regulation | Wang R.,Fuyang Teachers College | Wang R.,Key Laboratory of Embryo Development and Reproductive Regulation | And 2 more authors.
2011 International Conference on Remote Sensing, Environment and Transportation Engineering, RSETE 2011 - Proceedings | Year: 2011

The objective of this research was to evaluate the influence of body weight on survival and reproductive parameters of female adults of Propylaea japonica (Coleoptera: coccinellidae). Treatments were represented by females of this predator with the following mean weight: 3.5±0.5 mg, 5.0±0.5mg, 6.5±0.5mg, 8.0±0.5mg and 9.0±0.5mg. Heavier females of P. japonica showed shorter preoviposition period while the number of eggs and of larvae per day presented a positive linear relationship with female weight of this predator. Longevity of females of P. japonica was similar between treatments with no tendency of variations as a function of body weight of these females. Since the objective of mass rearing facilities of predatory ladybugs is to produce the maximum possible number of larvae per day it is recommended to use females of P. japonica with weight above 8.0 mg. © 2011 IEEE.

Wu F.-R.,Fuyang Teachers College | Wu F.-R.,Key Laboratory of Embryo Development and Reproductive Regulation | Li D.-K.,Fuyang Teachers College | Li D.-K.,Key Laboratory of Embryo Development and Reproductive Regulation | And 9 more authors.
Genes and Genomics | Year: 2016

Abtract: The plant Schisandra chinensis contains a phytoestrogens, a type of naturally occurring estrogens which have multiple functions in a number of biological processes. To investigate the correlation between phytoestrogens and epigenetic modification, especially the effect of phytoestrogens on DNA methylation, sexually healthy female mice were used as an animal model in the present study. Briefly, the total RNA and protein were isolated from the ovary of mice after 7-day oral administration of Schisandra chinensis extract (SCE), while distilled water was given to the animals in the control group. Real-time PCR, Western blotting, and enzyme activity assays were performed to examine the effect of the extract of S. chinensis on Dnmt1 transcription and activity. A promoter assay was further conducted in MCF cells (ER positive) to explore also the influence of SCE on Dnmt1 transcriptional activity. The results revealed that the mRNA and protein expression levels of mouse Dnmt1 were both significantly downregulated in the treated group. The transcription of Dnmt1 was suppressed by SCE and in the E2-added group also. Meanwhile the numbers of oocytes at different stages were increased in the treated group when compared by histological analyses with those in the control group. Taken together, the results indicated that, similarly to the action of estrogen, phytoestrogens affected Dnmt1 transcription in mammals, regulating the related gene expression and cell differentiation. The findings of our examination provided also basic data and understanding for the correlation between phytoestrogens and epigenetic modification. © 2016 The Genetics Society of Korea and Springer-Science and Media

Wu F.R.,Fuyang Teachers College | Wu F.R.,Key Laboratory of Embryo Development and Reproductive Regulation | Ding B.,Fuyang Teachers College | Ding B.,Key Laboratory of Embryo Development and Reproductive Regulation | And 9 more authors.
Gene | Year: 2012

Ifrg15 is a newly identified interferon alpha responsive gene and is implicated in a wide variety of physiological roles in mammals. In the present study, multiple alignments of the deduced amino acids of 10 eutherian mammalian IFRG15/Ifrg15s isolated from open genomic database revealed that they were highly conserved. Real-time PCR showed that mouse Ifrg15 mRNA was expressed in MII stage oocytes and preimplantation embryos, and its highest value peaked at the stage of mouse blastocysts. To understand the effect of three development-related genes on the promoter activity of mouse Ifrg15, promoter analysis using luciferase assays in COS-7 cells were performed. The results showed that the transcription of mouse Ifrg15 was suppressed by Oct4 and Nanog when transfected with the longest Ifrg15 promoter reporter gene. After the relatively shorter promoters were co-transfected with Oct4, c-Myc and Nanog, the relative luciferase activities of Ifrg15 were gradually increased. These in vitro results data and expression profiles of Ifrg15 as revealed by real-time PCR partly indicated that Ifrg15 transcription might be either potentially regulated or dependent on the post-transcriptional effects of IFN-α mediated by the three genes indirectly. Our data suggested that the mouse Ifrg15 might interact with these key development-related genes and play significant roles on the mouse preimplantation embryos development, especially for the development of mouse blastocysts. © 2012 Elsevier B.V.

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