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Yong L.,Key Laboratory of Embryo Development | Yong L.,Fuyang Teachers College | Biao D.,Key Laboratory of Embryo Development | Biao D.,Fuyang Teachers College | And 10 more authors.
Journal of Animal and Veterinary Advances | Year: 2012

Oocytes grow and obtain the ability of resume meiosis and maturation during follicular growth. Many regulatory mechanisms play a role in this process. DNA methylation and chromatin configuration are important regulatory mechanisms of gene transformation. The dynamic transformation of DNA methylation and chromatin configuration should be researched during follicular growth. Laser 3D sequence scanning and quantitative analysis fluorescence were used to discover the intensity and position of DNA methylation in porcine oocytes from different diameter follicles. The total fluorescence intensity (9374±2886 vs. 2703±473) and area (40914±7264 vs. 18862±2216) reduced during the follicular growth but the average fluorescence intensity (0.22±0.03 vs. 0.17±0.16) had no significant difference in <1 and >3mm group although reduced in 1 -3mm group (0.13±0.04). Confocal microscopy was also used to compare the relationship between DNA methylation and chromatin configuration. The position of DNA methylation matched chromatin configuration well in most oocytes but researchers found some different situations that need to be further researched. © 2012 Medwell Journals. Source


Yong L.,Fuyang Teachers College | Yong L.,Key Laboratory of Embryo Development | Biao D.,Fuyang Teachers College | Biao D.,Key Laboratory of Embryo Development | And 10 more authors.
Journal of Animal and Veterinary Advances | Year: 2012

There is a lack of quantitive and objective method study the chromatin configuration of mammalian oocytes. This study attempts to solve this problem. The Germinal Vesicle (GV) chromatin was stained with Hoechst33342 and photos were taken under the fluorescence microscope. The photos are then processed and the Integral Optical Density (IOD) of fluorescent areas and areas of germinal vesicle are measured. The researchers regard the IODs of fluorescent areas as Aggregation Degree (AD) and the ratio of fluorescent areas to the areas of GV as Spatial Distribution (SD). According to the traditional classification method of chromatin configuration in mouse, pig and goat, the researchers divided the photos of chromatin into several groups and calculated the AD, SD and IOD of chromatin configuration in these three species. The conclusions are as follows: quantitative fluorescence analysis technology established in this study can be applied in studying the chromatin configuration of mammalian oocytes. All the oocytes with poor capability to resume meiosis and support embryo development have a common feature that the value of AD is the smallest and the value of SD is the biggest in their own species. SD is a key parameter of chromatin configuration in relation to transcription. Transcription continued when SD>0.4 and ceased when SD<0.3. The value of AD increased and the value of SD decreased when the capabilities of the oocytes to resume meiosis and to support embryo development were enhanced. The value of IOD does not vary in healthy oocytes during the follicular growth stage. © Medwell Journals, 2012. Source

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