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Zhang H.,Jiangsu University | Zhang H.,Shanghai Academy of Agricultural science | Zhang H.,Key Laboratory of Edible Fungi Resources and Utilization South | Zhou J.,Jiangsu University | And 5 more authors.
Journal of Agricultural Science and Technology | Year: 2016

Lipases are known to have important functions in many physiological processes in plants. Here, we cloned a lipase gene via Rapid Amplification of cDNA Ends (RACE) technique from Brassica napus L., designated as BnDIL1 (B. napus Desiccation-Induced Lipase 1). The lipase enzyme activity was confirmed by estimating the lipase activity and reduced lipids content in Saccharomyces cerevisiae (pep4) transformant. Two B. napus lines with different oil contents were employed to examine the transcription profiles of BnDIL1 during the processes of seed morphogenesis, maturation, dormancy, pregermination and germination. The transcription level of lipid degradation pathway was enhanced during the processes of seed maturation, dormancy, pregermination and germination, and was higher in seeds of low oil-contents line than that of high oil-contents line. However, BnDIL1 was significantly activated when seed desiccation started. Both “slow desiccation” and “fast desiccation” treatments on seedlings dramatically activated the transcription of BnDIL1, while only “slow desiccation” stress, which would induce the cell apoptosis, significantly activated the transcription of lipid degradation gene. This result demonstrated that BnDIL1 in B. napus was desiccation stress dependent gene rather than fatty acids degradation gene. © 2016, Tarbiat Modares University. All rights reserved. Source


Zhou J.,Shanghai Institute of Technology | Zhou J.,Jiangsu Yurun Meat Group Co. | Han Y.,Jiangsu Yurun Meat Group Co. | Zhuang H.,Shanghai Academy of Agricultural science | And 3 more authors.
Food Analytical Methods | Year: 2015

In this paper, extraction of sauced duck neck meat volatile compounds by solid-phase microextraction (SPME) was optimized. Three extraction temperatures (30, 45, 60 °C), three extraction times (15, 30, 45 min), and three different fiber coatings (divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS), polydimethylsiloxane/divinylbenzene (PDMS/DVB), and polydimethylsiloxane (PDMS)) were assayed. There were 82 volatile compounds identified and quantified, which belonged to nine families of chemicals including alcohols, acids, esters, ketones, aldehydes, aromatic compounds, heterocycles, monoterpenes, oxygenated monoterpenes, and sesquiterpenes. The major volatile compounds of sauced duck neck came from spices addition, oxidation, Maillard reaction, etc. Almost all the compounds were extracted by the DVB/CAR/PDMS fiber at 45 °C, and the optimal time of extraction in this case was 15 min, during which DVB/CAR/PDMS fiber reached equilibration. Among the fibers tested, DVB/CAR/PDMS fiber favored the extraction of a wide range of compounds with low and high boiling point. On the other hand, PDMS/DVB fiber absorbed more polar and high retention index (RI) aromatic compounds. © 2014, Springer Science+Business Media New York. Source


Wu L.,Shanghai Academy of Agricultural science | Wu L.,Key Laboratory of Edible Fungi Resources and Utilization South | van Peer A.,Iwate Biotechnology Research Center | Song W.,Shanghai Academy of Agricultural science | And 13 more authors.
Gene | Year: 2013

During the life cycle of heterothallic tetrapolar Agaricomycetes such as Lentinula edodes (Berk.) Pegler, the mating type system, composed of unlinked A and B loci, plays a vital role in controlling sexual development and resulting formation of the fruit body. L. edodes is produced worldwide for consumption and medicinal purposes, and understanding its sexual development is therefore of great importance. A considerable amount of mating type factors has been indicated over the past decades but few genes have actually been identified, and no complete genetic structures of L. edodes B mating-type loci are available. In this study, we cloned the matB regions from two mating compatible L. edodes strains, 939P26 and 939P42. Four pheromone receptors were identified on each new matB region, together with three and four pheromone precursor genes in the respective strains. Gene polymorphism, phylogenetic analysis and distribution of pheromone receptors and pheromone precursors clearly indicate a bipartite matB locus, each sublocus containing a pheromone receptor and one or two pheromone precursors. Detailed sequence comparisons of genetic structures between the matB regions of strains 939P42, 939P26 and a previously reported strain SUP2 further supported this model and allowed identification of the B mating type subloci borders. Mating studies confirmed the control of B mating by the identified pheromone receptors and pheromones in L. edodes. © 2013 Elsevier B.V. Source


Li T.,Shanghai Academy of Agricultural science | Li T.,Shanghai University | Yang Y.,Shanghai Academy of Agricultural science | Yang Y.,Key Laboratory of Edible Fungi Resources and Utilization South | And 12 more authors.
International Journal of Biological Macromolecules | Year: 2015

Nine polysaccharide fractions were obtained from the fruiting bodies, submerged mycelia, and solid state fermented products of Phellinus baumii using different concentrations of ethanol precipitation. The chemical characteristics and in vitro immunological activities of the nine polysaccharide fractions were compared and studied. Results indicated that the fractions precipitated with 50% ethanol had higher yields of polysaccharides and submerged mycelia contributed to high extraction yields of polysaccharides and possessed higher polysaccharide contents. HPSEC-MALLS-RI analysis showed that the molecular weight (Mw) of polysaccharide fractions from these three materials decreased with the increasing of precipitated ethanol concentration. The Mw of fruiting body polysaccharide fractions ranged from 1.98×104Da to 1.89×106Da. Large-molecular-weight polysaccharides (from 2.11×106Da to 2.01×107Da) were found in submerged mycelia. Some lower-molecular-weight polysaccharide components were found in solid fermented products. Different culture methods contributed to significant differences in monosaccharide components and molar ratios. The 50% ethanol precipitated fractions exhibited more complexity on monosaccharide compositions comparing with fractions precipitated with 30% and 70% ethanol. Polysaccharide fractions derived from submerged mycelia exhibited higher macrophages stimulation activities. Submerged culture was found to be a suitable method to prepare active polysaccharides because of its short culture span and reasonable cost. © 2015 Elsevier B.V. Source


Xiong D.,Shanghai Academy of Agriculture Science | Xiong D.,Huazhong Agricultural University | Wang H.,Key Laboratory of Edible Fungi Resources and Utilization South | Wang H.,Shanghai Key Laboratory of Agricultural Genetics and Breeding | And 14 more authors.
Scientia Horticulturae | Year: 2014

The mating types of 124 and 88 single spore isolates, isolated at random from Volvariella volvacea strains V23 (. A mating type locus alleles A1+. A2) and PY (. A mating type locus alleles A3+. A4) respectively, were determined by molecular screening using four primer pairs designed according to the sequences of the A mating type genes (. HD1 and HD2) of the two parental strains. Of the single spore isolates from strain V23, 101were confirmed as homokaryons, 35 of which were mating type A1 and 66 mating type A2. The remaining 23 were heterokaryons carrying both A1 and A2 mating type loci. Of the single spore isolates from strain PY, 72 were confirmed as homokaryons, of which 41 were mating type A3 and 31 mating type A4. Sixteen were heterokaryons carrying both A3 and A4 mating type loci. Cross-breeding between 72 compatible pairs generated 58 hybrids, three of which were designated high quality hybrids based on agronomic traits determined by cultivation experiments. Our research has established that the molecular marker-assisted cross-breeding technique represents a practical and highly efficient system for generating improved V. volvacea cultivars. © 2014 Elsevier B.V. Source

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