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Qiu H.,China Agricultural University | Cheng G.,China Agricultural University | Xu J.,China Agricultural University | Xu J.,Key Laboratory of Development and Evaluation of the Chemical and Herbal Drugs for Animal Use | And 5 more authors.
Procedia in Vaccinology | Year: 2010

The aim of this study was to determine the effects of Astragalus Polysaccharide (APS) on associated Immunity Cells and Cytokines in the immunosuppressive dogs and its dose-effect correlation. One hundred two-month-old male Chinese Countryside Dogs were randomly assigned to five groups: Control group (CG), immunosuppressive group (IG), APS low dose group (50 mg/kg, LDG), APS median dose group (100 mg/kg, MDG), and APS high dose group (200 mg/kg, HDG), each group with twenty animals. After successfully established the dexamethasone-induced immunosuppressive models, with intravenous administer the CG and IG groups were daily dosed with saline, and the other three groups were daily dosed with APS for 7 days. On day 4 and 11 venous blood samples were collected and analyzed to determine the percentages of peripheral blood ANAE + T lymphocytes, CD4 +, CD8 + cells and CD4 +/CD8 + ratio; the phagocytic index and percentage of the peritoneal macrophages; and the contents of INF-γ and IL-2. After 7 days administration, the measured parameters as described above in three treated groups increased significantly (P<0.05). Our findings show that the dosage of 200 mg/kg APS can significantly enhance the cellular immune level of the immunosuppressive dogs. This study has provided evidence and basis for Astragalus polysaccharides development as companion animal health products as well as for its clinical application. © 2010 Elsevier B.V. All rights reserved.

Zhu X.-Y.,China Agricultural University | Cheng G.-L.,China Agricultural University | Liu F.-H.,China Agricultural University | Yu J.,China Agricultural University | And 5 more authors.
Archives of Pharmacal Research | Year: 2011

Heat stress (HS) may induce immunosuppression as well as inhibit the proliferation of lymphocytes. This study evaluated the effects on immune function of our prescription on splenic lymphocytes under HS as well as its compatibility. The effects of four herbal extracts from Agastache rugosa, Atractylodes lancea, Cortex Phellodendri, and Gypsum Fibrosum on heat treated splenic lymphocytes were investigated and the compatibility of the prescription was also explored by using the Taguchi method. This study revealed changes in proliferation by traditional Chinese medicines of splenic lymphocytes after HS. Proliferation in the HS group was significantly lower than the control group. Under HS, the effects of higher concentrations of Agastache rugosa (100 and 200 μg/mL), Atractylodes lancea (100 and 200 μg/mL), Cortex Phellodendri (50 and 100 μg/mL) and Gypsum Fibrosum (100 and 200 μg/mL) caused a significant increase on ConA/LPS-induced proliferation of lymphocytes than lower concentrations. We, therefore, conclude that the prescription of traditional Chinese medicines may recover splenic lymphocytes from the immunosuppression induced by HS. The Taguchi design, which allows rapid and high efficiency for the selection of the best conditions for our prescription on HS-treated splenic lymphocytes, demonstrated that Agastache rugosa (200 μg/mL), Atractylodes lancea (200 μg/mL), Cortex Phellodendri (100 μg/mL) and Gypsum Fibrosum (100 μg/mL) were the optimal conditions for the prescription. The validation experiment confirmed that our composition in optimum extraction conditions enhanced effects on ConA or LPS-stimulated lymphocytes under HS. The results showed that the Taguchi optimization approach is a suitable method for optimization of the composition of prescription. © 2011 The Pharmaceutical Society of Korea and Springer Netherlands.

Wang A.,China Agricultural University | Liu F.,China Agricultural University | Wang Z.,China Agricultural University | Jiang X.,Tech Lab Group | And 4 more authors.
Asian Journal of Animal and Veterinary Advances | Year: 2011

The purpose of this study was to establish a model of avian viral disease for antiviral drug discovery and development. A Chinese IBDV strain BC6185 was inoculated into 5-week-old specific-pathogen-free (SPF) male White Leghorn chicken and the clinicopathologic features of disease were compared with those reported before. Birds were monitored clinically and euthanized sequentially with collection of tissues. Tissues were examined by histopathology to observe microscopic lesions, by immunohistochemistry to detect viral protein, by electron microscope to observe ultrastructural changes of the plical epithelium of the bursa of Fabricius and by flow cytometry to detect apoptosis in bursal lymphocytes. Comparing of mean body weight gains and burs al weight indices between control group and virus inoculated groups, significant decreases are displayed. It was observed that the young chickens infected developed the typical histopathological changes of disease. The bursa of Fabricius revealed lesions characteristic of infectious bursal disease and virus particles identical to IBDV were found in the necrotic cells of the follicles. The mucosal surface of the bursal plica was erosion, ulceration and became smooth in the infected chicken groups. And the apoptotic cells were increased after 2 days of the virus inoculation. Morphologically, we clearly demonstrated that replication of IBDV in the bursa during the acute phase of infection and provided new information on the pathogenesis of IBDV. © 2011 Academic Journals Inc.

Zhang N.,CAU BUA TCVM Teaching and Research Team | Zhang N.,Key Laboratory of Development and Evaluation of the Chemical and Herbal Drugs for Animal Use | Guo S.,South China Agricultural University | Li H.,China Agricultural University | And 14 more authors.
Journal of Animal and Veterinary Advances | Year: 2012

Sijunzi decoction (SJZ), composed of Dangshen (Codonopsis pilosula Nannf.), Baizhu (Atractylodes macrocephala Koidz.), Filling (Tuckahoe) and Gancao (Radix glycyrrhiza) is well known as a classical Chinese traditional and herbal medicamentum aiming directly at spleen deficiency in Traditional Chinese Medicine (TCM). The present research is to evaluate the regulatory effect of SJZ on small intestinal T lymphocyte subsets differentiation in reserpine-induced spleen deficiency rats. Reserpine induced spleen deficiency rats were orally administrated with SJZ (0.2 mL/200 g, 0.6 mL/200 g and 1.0 mL/200 g, body weight) once a day for a period of 5 days. Clinical symptoms including body weight were observed every day, the small intestine damage and recovery by SJZ were detected by electron microscope scanning, small intestine lymphocytes were separated for T lymphocyte subsets detection by flow cytometry and T lymphocyte related cytokine expression was studied using quantitative real time PCR. Results showed that proper dose of SJZ can significantly promote the recovery of body weight and small intestine damage as well as regulate the differentiation of CD3 +, CD3 +CD4 + T lymphocyte subsets and the expression of Type 1 cytokines (IL-2, IFN-γ and IL10). Besides, a significant CD4 -CD8 - T lymphocyte subset was found in reserpine treated and high dose SJZ administrated rats. Data from this study proved that SJZ possess a significant promoting effect on the amelioration of spleen deficiency, CD4 + T lymphocyte and cytokines related with regulation of T lymphocyte differentiation would be key targets of SJZ on spleen deficiency. © Medwell Journals, 2012.

Zhang N.,China Agricultural University | Li J.,China Agricultural University | Li J.,Hebei Institute of Animal Science | Hu Y.,China Agricultural University | And 8 more authors.
Carbohydrate Polymers | Year: 2010

This study was conducted to evaluate the effects of astragalus polysaccharide (APS) on the immune responses of mice immunized with foot-and-mouth disease virus (FMDV) vaccine. BALB/c mice were randomly divided into five groups and 1 day before being immunized with FMDV vaccine, each group was orally administered APS at doses of 0, 0.125, 0.25, 0.5 and 1 mg, respectively. The cellular and humoral responses, including phagocytic capacity of peritoneal macrophage, the maturation of dendritic cells (DCs), splenic lymphocyte proliferation, cytokine production and antibody variation, were evaluated by a series of experiments after vaccination. The data showed that oral administration of APS significantly enhanced the phagocytic capacity of peritoneal macrophage, splenic lymphocyte proliferation, serum antibody titer, and the production of IL-4 and IL-10 in groups administered with 0.125-0.5 mg APS. The expression of CD40, CD86 and MHC-II on DCs was only slightly inhibited in mice given a low dose of APS, but was significantly enhanced in mice given higher doses. Furthermore, mice given the highest doses of APS showed markedly increased CD40 and MHC-II expression levels. These results suggest that orally administered APS could significantly enhance the efficacy of FMDV vaccination and has important implications for the further use of APS as a novel adjuvant. © 2010 Elsevier Ltd. All rights reserved.

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