Key Laboratory of Development and Application of Rural Renewable Energy

Chengdu, China

Key Laboratory of Development and Application of Rural Renewable Energy

Chengdu, China
SEARCH FILTERS
Time filter
Source Type

Zhao Y.,Central China Normal University | Wang Y.,Central China Normal University | Li D.H.,Central China Normal University | Deng Y.,Biogas Institute of Ministry of Agriculture | And 2 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2017

A Gram-stain-negative, non-motile, aerobic and rod-shaped bacterium, strain Ra1T, was isolated from the gut of a wood-feeding lower termite, Reticulitermes aculabialis. Phylogenetic analysis of 16S rRNA gene sequences showed that the strain was closely related to Chryseobacterium rigui JCM 18078T(96.7 % similarity). Growth was observed at 15-45 °C (optimum 30 °C), at pH 6.0-9.0 (optimum pH 8.0) and in the presence of 0-2 % (w/v) NaCl (optimum 0 %). The DNA G+C content of strain Ra1Twas 39.9 mol%. Cells contained menaquinone MK-6 as the sole respiratory quinone and the major fatty acids were iso-C15 : 0, iso-C17 : 0, summed feature 3 (comprising C16 : 1ω6c and/or C16 : 1ω7c) and summed feature 9 (comprising C16 : 010-methyl and/or iso-C17 : 1ω9c). The predominant polyamine was sym-homospermidine. The cellular polar lipids consisted of one phosphatidylethanolamine, three unidentified aminolipids, one unidentified phospholipid and one unidentified lipid. Based on phenotypic, genotypic and phylogenetic studies, it is concluded that strain Ra1Trepresents a novel species of the genus Chryseobacterium, for which the name Chryseobacterium reticulitermitis sp. nov. is proposed. The type strain is Ra1T(=CCTCC AB 2015431T=KCTC 52230T). © 2017 IUMS.


He M.-X.,China Institute of Technology | He M.-X.,Key Laboratory of Development and Application of Rural Renewable Energy | Wu B.,China Institute of Technology | Shui Z.-X.,China Institute of Technology | And 9 more authors.
Applied Microbiology and Biotechnology | Year: 2012

Furfural from lignocellulosic hydrolysates is the prevalent inhibitor to microorganisms during cellulosic ethanol production, but the molecular mechanisms of tolerance to this inhibitor in Zymomonas mobilis are still unclear. In this study, genome-wide transcriptional responses to furfural were investigated in Z. mobilis using microarray analysis. We found that 433 genes were differentially expressed in response to furfural. Furfural up- or down-regulated genes related to cell wall/membrane biogenesis, metabolism, and transcription. However, furfural has a subtle negative effect on Entner-Doudoroff pathway mRNAs. Our results revealed that furfural had effects on multiple aspects of cellular metabolism at the transcriptional level and that membrane might play important roles in response to furfural. This research has provided insights into the molecular response to furfural in Z. mobilis, and it will be helpful to construct more furfural-resistant strains for cellulosic ethanol production. © 2012 Springer-Verlag.


He M.-X.,China Institute of Technology | He M.-X.,Key Laboratory of Development and Application of Rural Renewable Energy | Wu B.,China Institute of Technology | Shui Z.-X.,China Institute of Technology | And 9 more authors.
Biotechnology for Biofuels | Year: 2012

Background: High tolerance to ethanol is a desirable characteristics for ethanologenic strains used in industrial ethanol fermentation. A deeper understanding of the molecular mechanisms underlying ethanologenic strains tolerance of ethanol stress may guide the design of rational strategies to increase process performance in industrial alcoholic production. Many extensive studies have been performed in Saccharomyces cerevisiae and Escherichia coli. However, the physiological basis and genetic mechanisms involved in ethanol tolerance for Zymomonas mobilis are poorly understood on genomic level. To identify the genes required for tolerance to ethanol, microarray technology was used to investigate the transcriptome profiling of the ethanologenic Z. mobilis in response to ethanol stress. Results: We successfully identified 127 genes which were differentially expressed in response to ethanol. Ethanol up- or down-regulated genes related to cell wall/membrane biogenesis, metabolism, and transcription. These genes were classified as being involved in a wide range of cellular processes including carbohydrate metabolism, cell wall/membrane biogenesis, respiratory chain, terpenoid biosynthesis, DNA replication, DNA recombination, DNA repair, transport, transcriptional regulation, some universal stress response, etc. Conclusion: In this study, genome-wide transcriptional responses to ethanol were investigated for the first time in Z. mobilis using microarray analysis.Our results revealed that ethanol had effects on multiple aspects of cellular metabolism at the transcriptional level and that membrane might play important roles in response to ethanol. Although the molecular mechanism involved in tolerance and adaptation of ethanologenic strains to ethanol is still unclear, this research has provided insights into molecular response to ethanol in Z. mobilis. These data will also be helpful to construct more ethanol resistant strains for cellulosic ethanol production in the future. © 2012 He et al.; licensee BioMed Central Ltd.


He M.X.,China Institute of Technology | He M.X.,Key Laboratory of Development and Application of Rural Renewable Energy | Wu B.,China Institute of Technology | Qin H.,China Institute of Technology | And 11 more authors.
Biotechnology for Biofuels | Year: 2014

Biosynthesis of liquid fuels and biomass-based building block chemicals from microorganisms have been regarded as a competitive alternative route to traditional. Zymomonas mobilis possesses a number of desirable characteristics for its special Entner-Doudoroff pathway, which makes it an ideal platform for both metabolic engineering and commercial-scale production of desirable bio-products as the same as Escherichia coli and Saccharomyces cerevisiae based on consideration of future biomass biorefinery. Z. mobilis has been studied extensively on both fundamental and applied level, which will provide a basis for industrial biotechnology in the future. Furthermore, metabolic engineering of Z. mobilis for enhancing bio-ethanol production from biomass resources has been significantly promoted by different methods (i.e. mutagenesis, adaptive laboratory evolution, specific gene knock-out, and metabolic engineering). In addition, the feasibility of representative metabolites, i.e. sorbitol, bionic acid, levan, succinic acid, isobutanol, and isobutanol produced by Z. mobilis and the strategies for strain improvements are also discussed or highlighted in this paper. Moreover, this review will present some guidelines for future developments in the bio-based chemical production using Z. mobilis as a novel industrial platform for future biofineries. © 2014 He et al.; licensee BioMed Central Ltd.


Shui Z.-X.,China Institute of Technology | Qin H.,China Institute of Technology | Wu B.,China Institute of Technology | Ruan Z.-Y.,Chinese Academy of Agricultural Sciences | And 9 more authors.
Applied Microbiology and Biotechnology | Year: 2015

Furfural and acetic acid from lignocellulosic hydrolysates are the prevalent inhibitors to Zymomonas mobilis during cellulosic ethanol production. Developing a strain tolerant to furfural or acetic acid inhibitors is difficul by using rational engineering strategies due to poor understanding of their underlying molecular mechanisms. In this study, strategy of adaptive laboratory evolution (ALE) was used for development of a furfural and acetic acid-tolerant strain. After three round evolution, four evolved mutants (ZMA7-2, ZMA7-3, ZMF3-2, and ZMF3-3) that showed higher growth capacity were successfully obtained via ALE method. Based on the results of profiling of cell growth, glucose utilization, ethanol yield, and activity of key enzymes, two desired strains, ZMA7-2 and ZMF3-3, were achieved, which showed higher tolerance under 7 g/l acetic acid and 3 g/l furfural stress condition. Especially, it is the first report of Z. mobilis strain that could tolerate higher furfural. The best strain, Z. mobilis ZMF3-3, has showed 94.84 % theoretical ethanol yield under 3-g/l furfural stress condition, and the theoretical ethanol yield of ZM4 is only 9.89 %. Our study also demonstrated that ALE method might also be used as a powerful metabolic engineering tool for metabolic engineering in Z. mobilis. Furthermore, the two best strains could be used as novel host for further metabolic engineering in cellulosic ethanol or future biorefinery. Importantly, the two strains may also be used as novel-tolerant model organisms for the genetic mechanism on the “omics” level, which will provide some useful information for inverse metabolic engineering. © 2015, Springer-Verlag Berlin Heidelberg.


Liu S.,China Agricultural University | Liu S.,Key Laboratory of Development and Application of Rural Renewable Energy | Wu S.,China Agricultural University | Zhang W.,China Agricultural University | And 3 more authors.
Nongye Jixie Xuebao/Transactions of the Chinese Society for Agricultural Machinery | Year: 2013

The microbial pretreatment of yellow corn stover and corn stover silage was achieved via the solid-state cultivation of white-rot fungi. Pretreatment effects on the biodegradability and subsequent anaerobic production of methane were investigated. The white-rot fungi pretreatment of yellow corn stover was accompanied by 38.3% cellulose, 42.2% hemicellulose, and 39.1% lignin degradation, while the degradation of corn stover silage was 9.9% cellulose, 23.2% hemicellulose, and 15.2% lignin. The reducing sugar yield of pretreatment yellow corn stover and corn stover silage after enzymatic hydrolysis was 195.8 mg/g and 67.7 mg/g respectively. These indicated much more composition of yellow corn stover was degradated, and more available composition retained in the corn stover silage after pretreated.The results showed that the total VS methane production of pretreated corn stover silage was 215.5 mL/g after 21 d anaerobic digestion, which increased by 29.2% more than the untreated ones and accounting 73.1% for 60 d total methane production. The biogas production of pretreated yellow corn stover was not increased. So microbial pretreatment of corn stover silage by white-rot fungi could increase substrate biodegradability, and further stimulate methane production.


Huang Y.,Biogas Institute of Ministry of Agriculture | Huang Y.,Key Laboratory of Development and Application of Rural Renewable Energy | Sun Y.,Biogas Institute of Ministry of Agriculture | Sun Y.,Key Laboratory of Development and Application of Rural Renewable Energy | And 8 more authors.
FEMS Microbiology Letters | Year: 2013

A novel thermophilic, anaerobic, keratinolytic bacterium designated KD-1 was isolated from grassy marshland. Strain KD-1 was a spore-forming rod with a Gram-positive type cell wall, but stained Gram-negative. The temperature, pH, and NaCl concentration range necessary for growth was 30-65 °C (optimum 55 °C), 6.0-10.5 (optimum 8.0-8.5), and 0-6% (optimum 0.2%) (w/v), respectively. Strain KD-1 possessed extracellular keratinase, and the optimum activity of the crude enzyme was pH 8.5 and 70 °C. The enzyme was identified as a thermostable serine-type protease. The strain was sensitive to rifampin, chloramphenicol, kanamycin, and tetracycline and was resistant to erythromycin, neomycin, penicillin, and streptomycin. The main cellular fatty acid was predominantly C15:0 iso (64%), and the G+C content was 28 mol%. Morphological and physiological characterization, together with phylogenetic analysis based on 16S rRNA gene sequencing identified KD-1 as a new species of a novel genus of Clostridiaceae with 95.3%, 93.8% 16S rRNA gene sequence similarity to Clostridium ultunense BST (DSM 10521T) and Tepidimicrobium xylanilyticum PML14T (= JCM 15035T), respectively. We propose the name Keratinibaculum paraultunense gen. nov., sp. nov., with KD-1 (=JCM 18769T =DSM 26752T) as the type strain. © 2013 Federation of European Microbiological Societies.


Wang Q.,Jiangsu University | Wang Q.,Key Laboratory of Development and Application of Rural Renewable Energy | Wei W.,Jiangsu University | Kingori G.P.,Jiangsu University | Sun J.,Jiangsu University
Cellulose | Year: 2015

Pretreatments of wheat straw by NaOH/urea solvent at low temperature were investigated. To understand the cell wall disruption during this low temperature process, and its impacts on enzymatic hydrolysis, morphology, cellulose crystal structure, and chemical properties were investigated by using the following instruments: optical microscopy, confocal laser scanning microscopy, Fourier transform infrared spectra, and X-ray diffraction. The results implied that the deconstruction of plant cell wall at low temperature was attributed to disruption of the hydrogen bonds in cellulose and solubilization of hemicellulose and lignin. Meanwhile, the pretreatment approach resulted in almost full recovery of cellulose, approximately 60 % of lignin and 70 % of xylan removal, respectively. It’s interesting to note that cellulose I crystal structure in the substrate pretreated at a solid loading of 10 % was partially changed to cellulose II structure, while wheat straw pretreated at a higher solid loading of 20 %, retained the cellulose I structure. Almost complete saccharification (>95 %) of cellulose in pretreated substrates was achieved at a relatively low cellulase loading of 10 FPU/g substrates within 48 h. The loss of xylan in pretreated substrate had a negative effect on the total sugar recovery. © 2015 Springer Science+Business Media Dordrecht


Zhu N.-M.,Biogas Institute of Ministry of Agriculture | Zhu N.-M.,Key Laboratory of Development and Application of Rural Renewable Energy | Chen M.,Southwest University of Science and Technology | Guo X.-J.,Biogas Institute of Ministry of Agriculture | And 3 more authors.
Journal of Hazardous Materials | Year: 2015

In recent years, a potential controversy has arisen that whether the metal speciation in solid matrix determined its electrokinetic (EK) removal efficiency or by contrast. In present study, Cu and Zn in anaerobic digestate were selected as candidates to investigate the relation between the species of metal and EK treatment. The obtained results show that the removal efficiency for each fraction decreased in the order as follows: exchangeable ≥ bound to carbonates > bound to Fe-Mn oxides > bound to organic matters » residual. For both Cu and Zn, their total removal performance was dependent on their dominant fraction in the digestate. A constant pH maintenance around the digestate via circulation of acid electrolyte is an optional operation because a strong acid atmosphere (pH < 2) around the digestate can be formed automatically as EK time elapses. Despite that many reactions occurred during EK process, the species distribution of Cu and Zn in the digestate determined their total EK removal efficiency essentially. © 2014 Elsevier B.V.


Zhu N.-M.,Biogas Institute of Ministry of Agriculture | Zhu N.-M.,Key Laboratory of Development and Application of Rural Renewable Energy | Qiang-Li,Biogas Institute of Ministry of Agriculture | Qiang-Li,Key Laboratory of Development and Application of Rural Renewable Energy | And 6 more authors.
Ecotoxicology and Environmental Safety | Year: 2014

In China, agricultural use of anaerobic digestate sludge is considered a concern due to high heavy metal content of the sludge. In this study, sequential extraction procedure (SEP) was conducted to determine metal speciation which affects release and mobility of metal significantly. The results of SEP showed that each heavy metal possessed different distribution characteristics. Cu mainly reacted with carboxyl functional group to form the fraction bound to organic matter. Zn and Mn were dominated in the fraction bound to Fe-Mn oxides and carbonates, respectively. Pb, Ni, Cr, Cd and As were present as the residual fraction. Examination of mobility factors (MFs) indicated that Zn, Pb, Ni, Mn and Cd were more mobile whereas Cr and As were immobilized in anaerobic digestate. Based on the results, it can be stated that Cu, Zn, Mn, Ni and Cd may be grouped as toxic and active components in sludge and should be regarded as the priority pollutants for elimination. Pb should be monitored in terms of its high mobility factors (MF). Cr and As, nevertheless, were the most stable components in sludge. © 2014 Elsevier Inc.

Loading Key Laboratory of Development and Application of Rural Renewable Energy collaborators
Loading Key Laboratory of Development and Application of Rural Renewable Energy collaborators