Key Laboratory of Detection for Veterinary Drug Residue and Illegal Additive

Laboratory of, China

Key Laboratory of Detection for Veterinary Drug Residue and Illegal Additive

Laboratory of, China
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Wang J.,China Agricultural University | Wang J.,Laboratory of Quality & Safety Risk Assessment for Animal Products on Chemical Hazards Beijing | Li Z.,Beijing General Station of Animal Husbandry | Sun F.,China Agricultural University | And 10 more authors.
Toxicology Reports | Year: 2017

The prediction of side-effects is a key issue in the REACH initiative on chemicals in the preclinical testing of drugs. The dermal irritation and skin sensitization toxicity potential of a new molecule, vitacoxib, were invested in rabbits and guinea pigs in compliance with the Organization for Economic Cooperation and Development guideline. To assess dermal irritation, rabbits were dermally attached to vitacoxib for 72 h or repeated application. The results showed that no adverse reactions such as erythema and edema were observed throughout the test. In skin sensitization test, guinea pigs were sensitized to vitaoxib, positive and negative article for 24 h. No sensitization reaction was shown in the vitacoxib and negative group whereas severe sensitization was observed in the positive group. Based on these findings, vitacoxib does not cause dermal irritation and skin sensitization toxicity, and seems to be safe for animal use. © 2017


Wang J.,China Agricultural University | Wang J.,Laboratory of Quality & Safety Risk Assessment for Animal Products on Chemical Hazards Beijing | Sun F.,China Agricultural University | Sun F.,Laboratory of Quality & Safety Risk Assessment for Animal Products on Chemical Hazards Beijing | And 9 more authors.
Regulatory Toxicology and Pharmacology | Year: 2017

Vitacoxib, is a newly developed coxibs NSAID (selective inhibitors of cyclooxygenase-2). To date, no experimental data have been published concerning its safety for use as an additive in the human diet. In the present study, we assessed the acute and sub-chronic toxicity of vitacoxib administered by gavage. The acute toxicity tests in Sprague Dawley (SD) rats and ICR mice demonstrated that vitacoxib at a dose of 5000 mg/kg BW failed to alter any of the parameters studied. In the 90-day sub-chronic toxicity test, vitacoxib was administered to SD rats at the doses of 0 (control), 5, 10, 20, 30, and 60 mg/kg BW. The results demonstrated that there were no significant differences for most indexes of sub-chronic toxicity throughout the experiment at the dose of 5–20 mg/kg BW, indicating no apparent dose-dependent. However, there were significant histopathology changes in the liver and kidney, and alterations in some biochemical parameters in the 60 mg/kg BW group. Based on these findings, the gavage LD50 was determined to be > 5000 mg/kg in SD rats and ICR mice, and the 90-day gavage no-observed-adverse-effect level (NOAEL) of vitacoxib was considered to be 20 mg/kg BW under the present study conditions. © 2017 Elsevier Inc.


Sun F.,China Agricultural University | Sun F.,Key Laboratory of Detection for Veterinary Drug Residue and Illegal Additive | Sun F.,Laboratory of Quality & Safety Risk Assessment for Animal Products on Chemical Hazards Beijing | Wang J.,China Agricultural University | And 10 more authors.
Journal of Veterinary Pharmacology and Therapeutics | Year: 2017

The pharmacokinetics and bioavailability of butafosfan in piglets were investigated following intravenous and intramuscular administration at a single dose of 10 mg/kg body weight. Plasma concentration–time data and relevant parameters were best described by noncompartmental analysis after intravenous and intramuscular injection. The data were analyzed through WinNolin 6.3 software. After intravenous administration, the mean pharmacokinetic parameters were determined as T1/2λz of 3.30 h, Cl of 0.16 L kg/h, AUC of 64.49 ± 15.07 μg h/mL, Vss of 0.81 ± 0.44/kg, and MRT of 1.51 ± 0.27 h. Following intramuscular administration, the Cmax (28.11 μg/mL) was achieved at Tmax (0.31 h) with an absolute availability of 74.69%. Other major parameters including AUC and MRT were 48.29 ± 21.67 μg h/mL and 1.74 ± 0.29 h, respectively. © 2016 John Wiley & Sons Ltd


Sun F.,China Agricultural University | Sun F.,Key Laboratory of Detection for Veterinary Drug Residue and Illegal Additive | Sun F.,Laboratory of Quality & Safety Risk Assessment for Animal Products on Chemical Hazards Beijing | Fan R.,China Agricultural University | And 11 more authors.
Journal of Veterinary Pharmacology and Therapeutics | Year: 2017

The pharmacokinetic characteristics of valnemulin in layer chickens were studied after single intravenous, intramuscular, and oral administration at a dose of 15 mg/kg body weight. Plasma samples at certain time points were collected and the drug concentrations in them by ultra high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS). The concentration–time data for each individual were plotted by noncompartmental analysis for the whole three routes. Following intravenous administration, the plasma concentration showed tiny fluctuation. The elimination half-life (T1/2λz), total body clearance (Cl), and area under the plasma concentration–time curve (AUC) were 1.85 ± 0.43 h, 2.2 ± 0.9 L/h, and 7.52 ± 2.46 μg·h/mL, respectively. Following intramuscular administration, the peak concentration (Cmax, 1.40 ± 0.43 μg/mL) was achieved at the time of 0.34 h. A multiple-peak phenomenon existed after oral administration, and the first peak and secondary peak were at 10 min and during 2–4 h, respectively, while the tertiary peak appeared during 5–15 h. The bioavailability (F %) for intramuscular and oral administration was 68.60% and 52.64%, respectively. In present study, the detailed pharmacokinetic profiles showed that this drug is widely distributed and rapidly eliminated, however has a low bioavailability, indicating that valnemulin is likely to be a favorable choice in the clinical practice. © 2017 John Wiley & Sons Ltd


Wang J.,China Agricultural University | Wang J.,Laboratory of Quality & Safety Risk Assessment for Animal Products on Chemical Hazards Beijing | Zhao T.,China Agricultural University | Zhao T.,Laboratory of Quality & Safety Risk Assessment for Animal Products on Chemical Hazards Beijing | And 12 more authors.
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2017

Vitacoxib is an imidazole derivative and the novel COX-2 selective inhibitor to be marketed for veterinary use as nonsteroidal anti-inflammatory drugs. No analytical assay to quantify vitacoxib in equine plasma samples has been published to date. In the current study, we aim to develop and validate a brief, quick and sensitive UPLC–MS/MS method for quantification of vitacoxib in equine plasma samples. Plasma samples were precipitated with methyl tert-butyl ether. The Phenomenex column (Kinetex 50 × 2.1 mm i.d. particle size = 2.6 μm, C18, 100 Å) at 25 °C was used in chromatographic separation with mobile phase consisting of acetonitrile and water (containing 0.1% formic acid) at flow rate of 0.4 mL/min. Vitacoxib and internal standard (IS, celecoxib) were detected under the multiple-reaction monitoring mode by mass spectrometer with ESI+ (m/z 347.9/269.03 for vitacoxib and m/z 382.0/362.0 for IS, respectively). The curve concentration range of was 0.5–500 ng/mL with a lower limit of quantification 0.5 ng/mL (r2 = 0.996309) in equine plasma samples. The selectivity, precision, recovery, accuracy, matrix effect and stability under various conditions were conformed to the acceptance requirements. Pharmacokinetic studies of vitacoxib in horses via oral administration (0.1 mg/kg) demonstrated that the procedure was fully validated and successfully. A meaningful basis for assessing the vitacoxib or clinical applications of vitacoxib to horse is provided in the present study. © 2017 Elsevier B.V.


Yang S.,China Agricultural University | Yang S.,Key Laboratory of Detection for Veterinary Drug Residue and Illegal Additive | Shi W.,China Agricultural University | Shi W.,Key Laboratory of Detection for Veterinary Drug Residue and Illegal Additive | And 15 more authors.
Journal of Agricultural and Food Chemistry | Year: 2014

Valnemulin, a semisynthetic pleuromutilin derivative related to tiamulin, is broadly used to treat bacterial diseases of animals. Despite its widespread use, metabolism in animals has not yet been fully investigated. To better understand valnemulin biotransformation, in this study, metabolites of valnemulinin in in vitro and in vivo rats, chickens, swines, goats, and cows were identified and elucidated using ultraperformance liquid chromatography-quadrupole/time-of-flight hybrid mass spectrometry (UPLC-Q/TOF-MS). As a result, there were totally 7 metabolites of valnemulin identified in vitro and 75, 61, and 74 metabolites detected in in vivo rats, chickens, and swines, respectively, and the majority of metabolites were reported for the first time. The main metabolic pathways of valnemulin were found to be hydroxylation in the mutilin part (the ring system) and the side chain, oxidization on the sulfur of the side chain to form S-oxides, hydrolysis of the amido bond, and acetylization in the amido of the side chain. In addition, hydroxylation in the mutilin part was proposed to be the primary metabolic route. Furthermore, the results revealed that 2β-hydroxyvalnemulin (V1) and 8α-hydroxyvalnemulin (V2) were the major metabolites for rats and swines and S-oxides (V6) in chickens. © 2014 American Chemical Society.


Yang S.,China Agricultural University | Yang S.,Key Laboratory of Detection for Veterinary Drug Residue and Illegal Additive | Li Y.,China Agricultural University | Li Y.,Key Laboratory of Detection for Veterinary Drug Residue and Illegal Additive | And 11 more authors.
Journal of Agricultural and Food Chemistry | Year: 2013

In the present study, metabolites of T-2 toxin in in vivo and in vitro systems of Wistar rats were identified and elucidated by ultraperformance liquid chromatography-quadrupole/time-of-flight tandem mass spectrometry (UPLC-Q/TOF-MS). Expected and unexpected metabolites were detected by MetabolynxXS software, which could automatically compare MS E data from the sample and control. A total of 19 metabolites of T-2 toxin were identified in this research, 9 of them being novel, which were 15-deacetyl-T-2, 3′-OH-15-deacetyl-T-2, 3′,7-dihydroxy-T-2, isomer of 3′,7-dihydroxy-T-2, 7-OH-HT-2, isomer of 7-OH-HT-2, de-epoxy-3′,7-dihydroxy-HT-2, 9-OH-T-2, and 3′,9-dihydroxy-T-2. The results showed that the main metabolic pathways of T-2 toxin were hydrolysis, hydroxylation, and de-epoxidation. In addition, the results also revealed one novel metabolic pathway of T-2 toxin, hydroxylation at C-9 position, which was demonstrated by the metabolites 9-OH-T-2 and 3′,9-dihydroxy-T-2. In addition, hydroxylation at C-9 of T-2 toxin was also generated in in vitro of liver systems. Interestingly, several metabolites of hydroxylation at C-7 of T-2 toxin were also detected in in vivo male Wistar rats, but they were not found in in vivo female rats and in in vitro systems of Wistar rats. © 2013 American Chemical Society.


Wang C.,China Agricultural University | Wang C.,Key Laboratory of Detection for Veterinary Drug Residue and Illegal Additive | Wang Z.,China Agricultural University | Wang Z.,Key Laboratory of Detection for Veterinary Drug Residue and Illegal Additive | And 4 more authors.
Molecules | Year: 2012

Due to the widespread use and potential toxicity of avermectins (AVMs), multi-residue monitoring of AVMs in edible tissues, especially in milk, has become increasingly important. With the aim of developing a broad-selective immunoassay for AVMs, a broad-specific monoclonal antibody (Mab) was raised. Based on this Mab, a homologous indirect enzyme-linked immunosorbent assay (ELISA) for the rapid detection of AVMs in milk was developed. Under the optimized conditions, the IC 50 values in assay buffer were estimated to be 3.05 ng/mL for abamectin, 13.10 ng/mL for ivermectin, 38.96 ng/mL for eprinomectin, 61.00 ng/mL for doramectin, 14.38 ng/mL for emamectin benzoate. Detection capability (CCβ) of the ELISA was less than 5 ng/mL and 2 ng/mL in milk samples prepared by simple dilution and solvent extraction, respectively. The optimized ELISA was used to quantify AVMs in milk samples spiked at different amounts. The mean recovery and coefficient of variation (CV) were 95.90% and 15.42%, respectively. The Mab-based ELISA achieved a great improvement in AVMs detection. Results proved this broad-selective ELISA would be useful for the multi-residue determination of AVMs in milk without purification process. © 2012 by the authors.


PubMed | Key Laboratory of Detection for Veterinary Drug Residue and Illegal Additive, China Institute of Veterinary Drugs Control and China Agricultural University
Type: | Journal: Scientific reports | Year: 2015

Mycoplasma gallisepticum is a significant pathogenic bacterium that infects poultry, causing chronic respiratory disease and sinusitis in chickens and turkeys, respectively. M. gallisepticum infection poses a substantial economic threat to the poultry industry, and this threat is made worse by the emergence of antibiotic-resistant strains. The mechanisms of resistance are often difficult to determine; for example, little is known about antibiotic resistance of M. gallisepticum at the proteome level. In this study, we performed comparative proteomic analyses of an antibiotic (tylosin)-resistant M. gallisepticum mutant and a susceptible parent strain using a combination of two-dimensional differential gel electrophoresis and nano-liquid chromatography-quadrupole-time of flight mass spectrometry. Thirteen proteins were identified as differentially expressed in the resistant strain compared to the susceptible strain. Most of these proteins were related to catalytic activity, including catalysis that promotes the formylation of initiator tRNA and energy production. Elongation factors Tu and G were over-expressed in the resistant strains, and this could promote the binding of tRNA to ribosomes and catalyze ribosomal translocation, the coordinated movement of tRNA, and conformational changes in the ribosome. Taken together, our results indicate that M. gallisepticum develops resistance to tylosin by regulating associated enzymatic activities.

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