Song Y.,Laboratory of Crop Heterosis and Utilization |
Song Y.,Beijing Key Laboratory of Crop Genetic Improvement |
Song Y.,Key Laboratory of Crop Genetic Improvement and Genome |
Song Y.,China Agricultural University |
And 24 more authors.
A molecular linkage map was developed for the tetraploid orchardgrass (Dactylis glomerata L.) from an F 1 pseudo-testcross population of 76 individuals derived from the cross between two individual plants of Akimidori, a Japanese cultivar and Loke, a Swedish cultivar. Two simple sequence repeat (SSR)-based parental linkage maps were constructed from 314 polymorphic homologous SSR markers derived from the same species. The Akimidori map consisted of 24 linkage groups (LGs) and had a total length of 562cM, with 168 loci and an average map density of 3.3cM. Five homologous LGs were detected on this map. The Loke map consisted of 26 LGs and had a total length of 745cM, with 227 loci and an average map density of 3.3cM. Seven homologous LGs were detected on this map. A consensus map with seven homologous LGs was developed from these using TetraploidMap software based on the double-simplex (segregated as 3:1) and duplex (segregated as 5:1) markers. This is the first linkage map of the tetraploid Dactlysis and these maps will be useful for quantitative trait locus (QTL) analysis, marker-assisted selection and breeding for important traits in orchardgrass in the future. © 2011 The Authors. Grassland Science © 2011 Japanese Society of Grassland Science. Source