Key Laboratory of Crop Genetic Improvement and Biotechnology

Jinan, China

Key Laboratory of Crop Genetic Improvement and Biotechnology

Jinan, China
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Xing J.,Linyi Normal University | Wang B.,Linyi Normal University | Jia K.,Linyi Normal University | Wan S.,Shandong Academy of Agricultural Sciences | And 7 more authors.
African Journal of Biotechnology | Year: 2011

The plant Na +/H + antiporter gene plays a major role in salt tolerance. One Na +/H + antiporter (AhNHX1) gene was isolated from peanut (Arachis hypogaea) in the present work. The full-length cDNA of AhNHX1 was 2,331 bp, which contains a complete ORF of 1,620 bp. The deduced protein sequence contains 546 amino acids with a molecular mass of 58.8 KDa and a pI of 7.23. The amino acid sequence of the AhNHX1 shares high similarity (shows more than 81.89% identity) with those of the previously isolated NHX1 from other plants. Meanwhile, the AhNHX1 protein has twelve putative transmembrane domains, and the conserved amiloride-binding sites ( 84LFFIYLLPPI 93) were found in its N-terminal. Also, the expression of AhNHX1 was tissue-specific under different level NaCl treatments. Under 50 and 100 mM NaCl treatments, its expression showed higher in roots and lower in stems and leaves relative to control plants, respectively. In addition, its expression was time-specific, such that under 150 mM NaCl treatment, its expression usually increased and reached to a stable level in roots and leaves after 24 h but in stems after 48 h, respectively. These results implied that the AhNHX1 plays an important role under salt stress in peanut. © 2011 Academic Journals.


Zhang Y.-J.,Shandong Academy of Agricultural Sciences | Zhang Y.-J.,Key Laboratory of Crop Genetic Improvement and Biotechnology | Zhang Y.-J.,Linyi Normal College | Yang J.-S.,Shandong Academy of Agricultural Sciences | And 10 more authors.
Plant Biology | Year: 2011

A known Arabidopsis cDNA clone, the CRT/DRE binding factor 1 (CBF1), was isolated and introduced into tomato plants. CBF1 is a member of the CBF gene family related to low temperature and enhanced low temperature tolerance in plants. In the present work, transcripts of CBF1 could be detected in transgenic tomato leaves, and the photochemical efficiency of PSII (Fv/Fm) and oxidisable P700 in the transgenic tomato over-expressing CBF1 were higher than in non-transformed plants under low temperature stress at low irradiance. Similarly, higher activity of superoxide dismutase (SOD), higher non-photochemical quenching (NPQ), and lower malondialdehyde (MDA) content were also detected in transgenic tomato leaves. These results suggest that CBF1 protein plays an important role in protection of PSII and PSI during low temperature stress at low irradiance. © 2010 German Botanical Society and The Royal Botanical Society of the Netherlands.


Chen G.,Shandong Normal University | Chen G.,Shandong Academy of Agricultural Sciences | Chen G.,Key Laboratory Of Crop Genetic Improvement And Biotechnology | Peng Z.Y.,Shandong Academy of Agricultural Sciences | And 7 more authors.
Research Journal of Biotechnology | Year: 2012

In this study, a full-length cDNA of the acyl-ACP thioesterase, AhFatA, was cloned from developing seeds of Arachis hypogaea L. by 3′-RACE. Sequence analysis showed that the open reading frame encodes a peptide of 372 amino acids and showed 50-70% identity with FatA from other plants. Real-time quantitative PCR analysis revealed that AhFatA was expressed in all tissues of A. hypogaea L., but most strongly in the immature seeds harvested at 60 days after pegging. Heterologous expression of AhFatA in Escherichia coli affected bacterial growth and changed the fatty acid profiles of the membrane lipid, resulting in directed accumulation towards palmitoleic acid and oleic acid. These results indicate that AhFatA is at least partially responsible for determining the high palmitoleic acid and oleic acid composition of E. coli.


Qin L.,Shandong Agricultural University | Qin L.,Shandong Academy of Agricultural Sciences | Qin L.,Key Laboratory of Crop Genetic Improvement and Biotechnology | Zhang Y.,Shandong Academy of Agricultural Sciences | And 8 more authors.
Shengtai Xuebao/ Acta Ecologica Sinica | Year: 2011

In agricultural systems, abiotic stresses, such as high light, high temperature and drought, are responsible for most of the reduction that differentiates yield potential from harvestable yield. As one of important oil crops in China, and even in the world, the high and stable yield of peanut (Arachis hypogaea L.) is very important to guarantee the food safety. Previous studies most focused on peanut yield, root activity, leaf area, net photosynthetic rate, chlorophyll content, activity of some enzymes, e. g. superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), and so on. However, effects of drought and high temperature stresses on damaging mechanisms of peanut photosystems have been seldom mentioned, and both coordination between photosytem II (PS II) and photosytem I (PS I) and response of electron transfer components to two kinds of stresses were unclear. In the present work, we try to investigate the effects of high temperature and drought stresses on PS IJ and PS I activity and coordination between PS II and PS I, and further to provide the theoretical basis to peanut cultivation. In the experiments, Luhua 14 was used as materials to investigate the responding mechanisms of peanut photosystems to high temperature and drought stresses. Detached leaves were exposed to high temperature (42 °C) under high irradiance (1200 μmol • m-2•s-1) (HH), drought (PEG-6000, 30%) under high irradiance (1200 μmol • m-2•s-1) (DH), and high irradiance (1200 μmol•m-2 • s-1) (NH), respectively, and non-treatment leaves were as controls (CK). Relative to CK and NH, the maximal efficiency of PS II (Fv/Fm) and absorbance at 820 nm decreased greatly in peanut leaves under HH and DH stresses, accompanied by the increase of relative variable fluorescence at the J-step (Vj), the obvious decrease of absorption flux per excited CS (t = m) (ABS/CSm), trapped energy flux per CS (t = m) (TRo/CSm) and active RCs per CS (t = m) (RC/CSm) calculated from the chlorophyll fluorescence transient curve. The obvious increase of 1-qP and the xanthophyll cycle-dependent energy dissipation (NPQ) were also detected in peanut leaves under HH and DH stresses. Additionally, the activity of SOD decreased in peanut leaves under HH and DH stresses, accompanied by the increase of malonaldehyde (MDA) and permeability of plasma membrane. These results showed that severe photoinhibition of PS II and PS I in peanut leaves was induced by HH and DH stresses. However, K-step was not induced in the rapid chlorophyll induction curve, which implied that peanut oxygen evolving complex (OEC) was not sensitive to high temperature and drought stresses, and donor side of PS II reaction centers was more sensitive to high temperature and drought stresses relatively. The main factor caused the damage to peanut photosystems might be the accumulation of reactive oxygen species (ROS) induced by excess energy. First, the xanthophyll cycle could only dissipate part of excess energy; second, the water-water cycle could not dissipated energy efficiently under the stresses of HH and DH, which caused the accumulation of ROS greatly. HH and DH stresses had similar damaging effects on peanut photosystems, except that DH had more severe effect. Additionally, effects of HH and DH stresses on damaging site and damaging mechanisms were similar in peanut leaves.


Huang C.,Shandong Academy of Agricultural Sciences | Huang C.,Key Laboratory of Crop Genetic Improvement and Biotechnology | Picimbon J.F.,Shandong Academy of Agricultural Sciences | Picimbon J.F.,Key Laboratory of Crop Genetic Improvement and Biotechnology | And 8 more authors.
Russian Journal of Plant Physiology | Year: 2012

Most of conventional RNA extraction methods failed to extract highly pure and integral RNA from peanut seeds because peanut seeds are extremely rich in lipids, proteins, polysaccharides, and phenolic compounds. Here, we describe a new method, named Peanut Improved Modified RNA extraction method (PIMRNAext), using SDS, Tris-saturated phenol, NaCl, and sarkosyl during the extraction process, which are particularly successful for total RNA extraction from lipid- and polysaccharide-rich materials. The proposed PIMRNAext method is simple and fast. It requires only conventional reagents and is completed within 2 h. Using PIMRNAext gives very good yields of high quality peanut RNA. This method is about ten times more efficient than conventional methods, and the RNA produced by it is compatible with further molecular biology experiments, such as RT-PCR. We propose to use the PIMRNAext method to extract RNA from peanuts and peanut-like plant species not only for RT-PCR, but also for most molecular biology techniques that need copies of pure RNA, such as microarray or cDNA library construction. © 2012 Pleiades Publishing, Ltd.


Chu D.,Shandong Academy of Agricultural Sciences | Chu D.,Key Laboratory of Crop Genetic Improvement and Biotechnology | Zhang Y.J.,Chinese Academy of Agricultural Sciences | Wan F.H.,Chinese Academy of Agricultural Sciences
Florida Entomologist | Year: 2010

Bemisia tabaci (Gennadius) is an important agricultural pest worldwide. The pest is a species complex composed of numerous biotypes, among which biotypes B and Q are the 2 most invasive and widely distributed. Our previous study found that the ratio of the biotype Q has been increasing and displacement of biotypes B by Q has been occurring on cotton and eggplant in Shandong Province of China during the past several years. To determine whether biotype Q has been increasing on other hosts and possible displacement of biotypes has been occurring in the province as a whole, we further surveyed B. tabaci biotypes B and Q on cultivated and wild host species near cotton or eggplant fields in 7 locations of Shandong Province during 2005-2008 with cleavage amplified polymorphic sequence (CAPS) of the mtCOI (mitochondrial cytochrome oxidase subunit I) marker. This research showed biotype Q has been increasing on all kinds of host plants and the displacement of biotypes B by Q has been occurring in the province as a whole. The displacement mechanism should be further researched and such knowledge might guide the application of the insecticides or adjustment of the crops to effectively control the pest.


Chu D.,Shandong Academy of Agricultural Sciences | Chu D.,Key Laboratory of Crop Genetic Improvement and Biotechnology | Wan F.H.,Shandong Academy of Agricultural Sciences | Wan F.H.,Chinese Academy of Agricultural Sciences | And 4 more authors.
Environmental Entomology | Year: 2010

Certain biotypes of the Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) complex cause extensive damage and are important pests and virus vectors in agricultural crops throughout the world. Among the most invasive and well studied are the B and Q biotypes. Recent reports in Shandong Province, China, have indicated that the Q biotype was introduced there in ≈ 2005, whereas the B biotype has been established there for ∼10 yr. Even so, the present distribution of the two biotypes in Shandong has not been examined. The results of this study showed that the B and Q biotypes are both present in Shandong Province based on bar-coding using a ≈450-base fragment of the mitochondrial cytochrome oxidase I (mtCOI) gene. In addition, a B biotype-specific polymerase chain reaction primer pair that amplifies a ≈300 bp mtCOI fragment was designed and used to examine the biotype composition of B. tabaci in selected crops from six provincial locations, using the general mtCOI primers as an internal positive control for DNA quality. The results of this study indicated that the Q biotype was the predominant B. tabaci colonizing all of the crops in the study sites examined. This suggests that the Q biotype has displaced the B biotype in Shandong Province of China, which until now was the predominant biotype. This is the first report of the displacement of the B by the Q biotype in field grown crops in China, and in a locale where neither the B nor the Q biotype is native. We hypothesize that this phenomenon may have been exacerbated by the widespread use of neonicotinoid insecticides for whitefly control, given the sustained efficacy thus far of neonicotinoids against the B biotype, and their failure at times to effectively control the Q biotype. © 2010 Entomological Society of America.


Chu D.,Shandong Academy of Agricultural Sciences | Chu D.,Key Laboratory of Crop Genetic Improvement and Biotechnology | Tao Y.L.,Shandong Academy of Agricultural Sciences | Tao Y.L.,National Chung Hsing University | Chi H.,National Chung Hsing University
Journal of Economic Entomology | Year: 2012

In recent years, the dominant biotype of Bemisia tabaci (Gennadius) has shifted from biotype B to Q in many locations of China. Our field monitoring showed that B. tabaci biotype Q could be found on pepper Capsicum frutescens L. while biotype B could not be found on the plant. To study the role of the host plant in the displacement of biotype B by Q, the adult mortality, female fecundity, and adult emergence of both biotypes B and Q on different host combinations were studied using a model system involving pepper and eggplant. The results showed that pepper is not a preferred host for either biotype B or Q adults in comparison with eggplant. The negative impact of pepper to biotype B is, however, much greater than that to biotype Q. Because both the survival rates of the adult whitefly and the average number of emerged adults per female of biotype Q on pepper are higher than that of biotype B, biotype Q showed higher fitness in comparison with biotype B. Our results suggest that the existence and differential impact of a nonpreferred host might affect the population fitness and therefore could play a role in the displacement of biotype B in some regions. © 2012 Entomological Society of America.


Qin L.-Q.,Shandong Academy of Agricultural Sciences | Qin L.-Q.,Key Laboratory of Crop Genetic Improvement and Biotechnology | Qin L.-Q.,Shandong Agricultural University | Li L.,Shandong Agricultural University | And 10 more authors.
Photosynthetica | Year: 2011

To investigate damaging mechanisms of chilling and salt stress to peanut (Arachis hypogaea L.) leaves, LuHua 14 was used in the present work upon exposure to chilling temperature (4°C) accompanied by high irradiance (1,200 μmol m-2 s-1) (CH), salt stress accompanied by high irradiance (1,200 μmol m-2 s-1) (SH), and high-irradiance stress (1,200 μmol m-2 s-1) at room temperature (25°C) (NH), respectively. Additionally, plants under low irradiance (100 μmol m-2 s-1) at room temperature (25°C) were used as control plants (CK). Relative to CK and NH treatments, both the maximal photochemical efficiency of PSII (Fv/Fm) and the absorbance at 820 nm decreased greatly in peanut leaves under CH and SH stress, which indicated that severe photoinhibition occurred in peanut leaves under such conditions. Initial fluorescence (Fo), 1 - qP and nonphotochemical quenching (NPQ) in peanut leaves significantly increased under CH- and SH stress. Additionally, the activity of superoxide dismutase (SOD), one of the key enzymes of water-water cycle, decreased greatly, the accumulation of malondialdehyde (MDA) and membrane permeability increased. These results suggested that damages to peanut photosystems might be related to the accumulation of reactive oxygen species (ROS) induced by excess energy, and the water-water cycle could not dissipate energy efficiently under the stress of CH and SH, which caused the accumulation of ROS greatly. CH and SH had similar damaging effects on peanut photosystems, except that CH has more severe effects. All the results showed that CH- and SH stress has similar damaging site and mechanisms in peanut leaves. © 2011 Springer Science+Business Media B.V.

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