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Jing Z.,Sichuan Agricultural University | Jing Z.,Key Laboratory of Crop Ecophysiology | Xinfu C.,Sichuan Agricultural University | Weiguo L.I.U.,Sichuan Agricultural University | And 3 more authors.
Research on Crops

Alisma orientalis (Sam.) Juzep is a common traditional Chinese medicine. The objectives of this study were to analyze genotype × environment (GE) interaction on yield and quality of four cultivars in seven environments by additive main effects and multiplicative interaction (AMMI) model. Main effects due to environment (E), genotype (G), GE interaction as well as first three interaction principal component axes (IPCA 1 to 3) were found to be significant (P < 0.01). Cluster analysis divided the A. orientale into three main sub-regions and three genotypes. Genotype 2 and genotype 4 could be chosen as excellent resources from these wild resources and used them to breed new and high quality cultivars. Production in Dujiangyan had the best yield and quality output, the region should be acted as the main production region. This classification of locations could be useful in breeding for specific adaptability within sub-regions. Source

Liu J.-L.,Sichuan Agricultural University | Liu J.-L.,Key Laboratory of Crop Ecophysiology | Zheng S.-L.,Sichuan Agricultural University | Fan Q.-J.,Sichuan Agricultural University | And 5 more authors.
Asian Journal of Chemistry

A simple and quick method is described for the determination of ligustrazine in rhizomes of Ligusticum chuanxiong. The ligustrazine in the sample was extracted using methanol and analyzed by reversed-phase high performance liquid chromatography. Chromatography separation was performed using Agilent 1100 series HPLC system with a waters symmetry C18 column and isocratic elution with a mixture of three solvents: acetonitrile, methanol and 1 % aqueous acetic acid (20: 40: 40, v/v/v). The effluent was monitored using a VWD detector set at 294 nm. The average recoveries were 84.93 - 88.65 % (n = 3). The limits of detection and quantification were 0.044 and 0.133 μg mL-1, respectively. The method has been successfully applied to the analysis of L. chuanxiong samples. For 37 samples, the ligustrazine content varied from 0.22 to 1.06 mg g-1. Source

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