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Cai S.-H.,Guangdong Ocean University | Cai S.-H.,Guangdong Prov Key Laboratory Of Pathogenic Biology And Epidemiology For Aquatic Economic Animals | Cai S.-H.,Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes | Lu Y.-S.,Guangdong Ocean University | And 20 more authors.
Diseases of Aquatic Organisms | Year: 2013

The outer membrane proteins of Vibrio alginolyticus play an important role in the virulence of the bacterium and are potential candidates for vaccine development. In the present study, the ompW gene was cloned, expressed and purified. A DNA vaccine was constructed by inserting the ompW gene into a pcDNA plasmid. Crimson snapper Lutjanus erythropterus (Bloch) were injected intramuscularly with the recombinant plasmid pcDNA-ompW. The expression of the DNA vaccine was detected in gill, head kidney, heart, liver, spleen and injection site muscle of crimson snapper by RT-PCR 7 and 28 d post-vaccination. The ELISA results demonstrated that the DNA vaccine produced an observable antibody response in all sera of the vaccinated fish. In addition, crimson snapper immunized with the DNA vaccine showed a relative percentage survival (RPS) of 92.53%, indicating effective protection against V. alginolyticus infection. © 2013 Inter-Research. Source


Cai J.,Guangdong Ocean University | Cai J.,Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals | Cai J.,Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes | Li N.,Guangdong Ocean University | And 13 more authors.
Gaojishu Tongxin/Chinese High Technology Letters | Year: 2015

In this study, the cDNA of orange-spotted grouper (Epinephelus coioides) MHC (major histocompatibility complex) IIα gene (Ec-MHC IIα) was cloned. And its distribution in healthy tissue and temporal expression after Vibro alginolyticus infection were also investigated by quantitative real-time PCR. The Ec-MHC IIα cDNA was 2127bp in length, including an 834 bp 5'UTR, a 714 bp open reading frame, and a 579 bp 3'UTR. The deduced amino acid sequence contained a signal peptide, alpha 1 and alpha 2 domains, transmembrane region, connecting peptide and cytoplasmic domain. The results of quantitative real-time PCR showed that the grouper MHC IIα gene was highly expressed in gill, head kidney, liver, trunk kidney, brain, spleen and intestine, but weakly expressed in heart, skin and muscle. Moreover, Ec-MHC IIα expressions were significantly up-regulated in spleen (9.5 fold), intestine (4.3 fold), head kidney (3.4 fold), kidney (1.9 fold) after Vibrio alginolyticus infection. These data indicates that Ec-MHC IIα might play an important role in immune response to Vibrio alginolyticus infection. It is of referential significance to better understanding of the grouper's immunity against bacteria invasion. ©, 2015, Inst. of Scientific and Technical Information of China. All right reserved. Source


Bei W.,Guangdong Ocean University | Bei W.,Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals | Bei W.,Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes | Yishan L.,Guangdong Ocean University | And 8 more authors.
Israeli Journal of Aquaculture - Bamidgeh | Year: 2015

The main aim of this study was to demonstrate that the surface immunogenic protein (Sip) of the aquatic pathogen Streptococcus agalactiae is a potential candidate for vaccine development for Oreochromis niloticus. In this study, a Sip was identified. Alignment analysis indicated that the Sip protein was highly homologous with Sip proteins from other S. agalactiae isolated from humans (99%). SDS-PAGE procedure indicated that the Sip protein was successfully expressed in Escherichia coli BL21 (DE3). The recombinant Sip protein was purified by affinity chromatography, and the mouse anti-Sip serum was produced. Tilapia Oreochromis niloticus were vaccinated with Sip, and immunogenicity was confirmed by subsequent western blotting. Enzyme-linked immunosorbent assay (ELISA) analysis demonstrated that Sip produced an observable antibody response in all sera of the vaccinated fish. The relative percentage survival (RPS) value for the Sip vaccine was 90.62. Tilapia vaccinated with Sip produced specific antibodies, and were highly resistant to infection by the virulent S. agalactiae. These results indicate that Sip is an effective vaccine candidate against S. agalactiae for tilapia, Oreochromisniloticus. © 2015, Israeli Journal of Aquaculture - Bamidgeh. All rights reserved. Source

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