Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Insititutes

Zhanjiang, China

Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Insititutes

Zhanjiang, China

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Gan Z.,Guangdong Ocean University | Gan Z.,Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals | Gan Z.,Key Laboratory of Control for Disease of Aquatic Animals of Guangdong Higher Education Insititutes | Wang B.,Guangdong Ocean University | And 19 more authors.
Fish and Shellfish Immunology | Year: 2015

CD59, the major inhibitor of membrane attack complex, plays a crucial role in regulation of complement activation. In this paper, a CD59 gene of Nile tilapia, Oreochromis niloticus (designated as On-CD59) was cloned and its expression pattern under the stimulation of Streptococcus agalactiae was investigated. Sequence analysis showed main structural features required for complement-inhibitory activity were detected in the deduced amino acid sequence of On-CD59. In healthy Nile tilapia, the On-CD59 transcripts could be detected in all the examined tissues, with the most abundant expression in the brain. When immunized with inactivated S. agalactiae, there was a clear time-dependent expression pattern of On-CD59 in the skin, brain, head kidney, thymus and spleen, with quite different kinetic expressions. The assays for the complement-inhibitory activity suggested that recombinant On-CD59 protein had a species-selective inhibition of complement. Moreover, our works showed that recombinant On-CD59 protein may possess both binding activities to PGN and LTA and inhibiting activity of S.agalactiae. These findings indicated that On-CD59 may play important roles in the immune response to S.agalactiae in Nile tilapia. © 2015 Elsevier Ltd.

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